General information for g5312.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Ubiquitin-conjugating enzyme E2Q-like protein CG4502.
Parent gene
- The parent gene g5312 is located on chr_2: 8665425..8673233 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5312 | g5312.t1 | isoform | g5312.t1 | 8663011 | 8665425 |
| chr_2 | g5312 | g5312.t1 | exon | g5312.t1.exon1 | 8663011 | 8663258 |
| chr_2 | g5312 | g5312.t1 | cds | g5312.t1.CDS1 | 8663011 | 8663258 |
| chr_2 | g5312 | g5312.t1 | exon | g5312.t1.exon2 | 8663325 | 8663511 |
| chr_2 | g5312 | g5312.t1 | cds | g5312.t1.CDS2 | 8663325 | 8663511 |
| chr_2 | g5312 | g5312.t1 | exon | g5312.t1.exon3 | 8663589 | 8663727 |
| chr_2 | g5312 | g5312.t1 | cds | g5312.t1.CDS3 | 8663589 | 8663727 |
| chr_2 | g5312 | g5312.t1 | exon | g5312.t1.exon4 | 8665286 | 8665425 |
| chr_2 | g5312 | g5312.t1 | cds | g5312.t1.CDS4 | 8665286 | 8665425 |
| chr_2 | g5312 | g5312.t1 | TSS | g5312.t1 | NA | NA |
| chr_2 | g5312 | g5312.t1 | TTS | g5312.t1 | NA | NA |
Sequences
>g5312.t1 Gene=g5312 Length=714
ATGATGAAGGCAGTATTTAAAAAGTTATTTAAAAGCTCTCCAAAAGACAAAGTTGATGTT
GCAAAAAATAATGAACCGCAACAAGAGGGTACAAGTAGACAAAATGTTGTTCTTCCTAGT
CCTGCAAGAAAAGTCAAATGCAATACGATAGCGCCGAGTGGAGGTAAATCAAAATTGCCA
ATTGTACCAAAAGTTGACACCACAATCCGAGCCAAGCGCCTAATGAAAGAATTAAAAGAC
CTACAAAAACAACGCGACACTGAGGATAGAATATTTGAGGCTGAATTGATAAATGATAAT
CTATTTGAGTGGCATGTAAAGTTGTTTAAAATCGATCAGGAATCCCCACTGGCCCAAGAC
ATGATAGAATTGAATGTTGAATATATTTTGCTCCATCTCATCTTTCCTGACAATTTTCCA
TTTGCGCCGCCATTTATGCGAGTTGTTGAGCCGAGAATTGAAAAAGGTTACGTGATGGAA
GGCGGTGCAATTTGTATGGAGCTACTGACGCCTCGCGGTTGGGCATCAGCCTACACAATT
GAAGCCGTCCTAATGCAATTTGCTGCCAGTTTAGTCAAAGGTCAAGGAAGAATATGTCGT
AAGACGAAAGTGAACAAAGAATTTAATCGAAGAACGGCTGAGGAAGCATTTAGATCGCTT
GTAAAAACTCATGACAAATATGGATGGATAACAGCTCCAAAATCTGAAGGTTAA
>g5312.t1 Gene=g5312 Length=237
MMKAVFKKLFKSSPKDKVDVAKNNEPQQEGTSRQNVVLPSPARKVKCNTIAPSGGKSKLP
IVPKVDTTIRAKRLMKELKDLQKQRDTEDRIFEAELINDNLFEWHVKLFKIDQESPLAQD
MIELNVEYILLHLIFPDNFPFAPPFMRVVEPRIEKGYVMEGGAICMELLTPRGWASAYTI
EAVLMQFAASLVKGQGRICRKTKVNKEFNRRTAEEAFRSLVKTHDKYGWITAPKSEG
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g5312.t1 | CDD | cd00195 | UBCc | 72 | 192 | 4.32062E-27 |
| 6 | g5312.t1 | Coils | Coil | Coil | 64 | 91 | - |
| 5 | g5312.t1 | Gene3D | G3DSA:3.10.110.10 | Ubiquitin Conjugating Enzyme | 67 | 229 | 3.9E-51 |
| 9 | g5312.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 16 | 35 | - |
| 2 | g5312.t1 | PANTHER | PTHR24068:SF72 | UBIQUITIN-CONJUGATING ENZYME E2Q-LIKE PROTEIN 1 | 48 | 237 | 1.2E-74 |
| 3 | g5312.t1 | PANTHER | PTHR24068 | UBIQUITIN-CONJUGATING ENZYME E2 | 48 | 237 | 1.2E-74 |
| 1 | g5312.t1 | Pfam | PF00179 | Ubiquitin-conjugating enzyme | 73 | 221 | 1.5E-12 |
| 10 | g5312.t1 | ProSiteProfiles | PS50127 | Ubiquitin-conjugating enzymes family profile. | 72 | 192 | 16.251 |
| 8 | g5312.t1 | SMART | SM00212 | ubc_7 | 72 | 230 | 2.2E-9 |
| 4 | g5312.t1 | SUPERFAMILY | SSF54495 | UBC-like | 67 | 211 | 4.2E-32 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5312/g5312.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5312.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
- This transcript has been annotated with K10582 (ubiquitin-conjugating enzyme E2 Q)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
