General information for g5319.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Receptor-type guanylate cyclase Gyc76C.
Parent gene
- The parent gene g5319 is located on chr_2: 8733442..8733905 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5319 | g5319.t1 | isoform | g5319.t1 | 8728143 | 8733905 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon1 | 8728143 | 8728523 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS1 | 8728143 | 8728523 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon2 | 8728909 | 8728974 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS2 | 8728909 | 8728974 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon3 | 8729048 | 8729257 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS3 | 8729048 | 8729257 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon4 | 8729703 | 8729857 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS4 | 8729703 | 8729857 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon5 | 8729918 | 8730318 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS5 | 8729918 | 8730318 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon6 | 8730383 | 8730555 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS6 | 8730383 | 8730555 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon7 | 8730658 | 8730951 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS7 | 8730658 | 8730951 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon8 | 8731005 | 8731194 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS8 | 8731005 | 8731194 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon9 | 8731256 | 8731392 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS9 | 8731256 | 8731392 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon10 | 8731459 | 8731627 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS10 | 8731459 | 8731627 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon11 | 8731691 | 8732232 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS11 | 8731691 | 8732232 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon12 | 8732288 | 8732435 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS12 | 8732288 | 8732435 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon13 | 8732497 | 8732673 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS13 | 8732497 | 8732673 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon14 | 8732738 | 8733385 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS14 | 8732738 | 8733385 |
| chr_2 | g5319 | g5319.t1 | exon | g5319.t1.exon15 | 8733442 | 8733905 |
| chr_2 | g5319 | g5319.t1 | cds | g5319.t1.CDS15 | 8733442 | 8733905 |
| chr_2 | g5319 | g5319.t1 | TTS | g5319.t1 | 8733990 | 8733990 |
| chr_2 | g5319 | g5319.t1 | TSS | g5319.t1 | NA | NA |
Sequences
>g5319.t1 Gene=g5319 Length=4155
ATGTCTATTATACGAGCGTTCATCGTAACTCTCGTGGCATTTGCTACGATTTTTAACTTT
GTCCACGGAAGCCATCACATAAAAACGACGCTAAATGTTGGCTACTTGACAGCCATTAAA
GGCGATCTTAAAGATCGTCAAGGATTAGCAATTTCAGGTGCAATTCAAATGGCGCTTGAA
GAAATTAATAATGATCCAAAAATATTGCCAAATGTTAAATTAGAATTATGGTGGAATGAC
ACAAGAGGCGATACAGTCATTGCGACACGTGCCATAACTGAAATGATTTGCAATGGTGTA
TCGACAATCTTTGGACCTGAAGGCACGTGCCATGTTGAAGCCATCGTTACACAAAGTCGA
AACATTCCCATGATTGCTTATAAATGTTCAGATTATAAAGCAAGCGCAATTCCAACTTTT
GCACGTACAGAACCACCTGATACTCAGGTGACCAAATCAGTGATCGCGCTTTTAAATTAT
TATCGTTGGAATAAGTTTTCGATAATCTATGAAGAGGCATGGGAAAGAGTGGCCGAGTCA
TTGAGCCAACAAGCGAAATCACATAATAAGACGATCAATCATAAAAAGCAGGTGGTCGAT
CGTCATAAATGTTGCGAGCAAAACATGGATTGCTGTCGACCAGGCTATTGGTACAATATC
ATTCAAAACACAATGAATCGAACAAGAATTTACATATTCTTGGGAACACCTGACGCTCTC
GTAGACATGATGGGATCGATGGAGTCAATTCAGTTGTTCGACAAAGGCGAATATATGGTG
ATTTTTGTCGATATGATGAGCTATTCTGCAGAAGAGGCAAAAAAATATTTGTATAAACTC
GAAAATTTTAGCAAATATAAATCATGCAACGAAATTGAAGGTTTTACAAAAAGAGCACGC
AGTTTACTTGTTGTTGTATCAACGCCACCAACCAAAAATTATGAAGAGTTTACTGATAAA
GTTCGCAAATATAACGAAAAGAAGCCATTTGAATTTAGAAATCCAAGTTTTCCCAATAGA
AAAAATTTTGTAAAATTTGTCTCAATTTATGCAGCTTATTTGTATGATGCTGTAAAATTA
TACGCAAATGCACTGCACAAATTGCTTAAGAAGGAAGAATTATCGCGACCGTTAACAGAT
GAAGTGATAAGAGAAATAGCGAGCAACGGAACTGCCATTATTAGTGCCATAATTCAAGAG
AAAAAATATCCAAGTATAACAGGTGCAGAGATATGGATAGATGAGAATGGCGATTCAGAA
GGAAATTTCTCAGTGCTTGCACTTAAAGAGTCTCAGTTCTCACCAAAAGACTCAAATTTC
ACATGTGATCGTCATATGATTACCGTTGCTACTTTTCAAGGTCGCAATAATAATAATTCA
CTACCAGAATATAAATTTAATAATATTCTTAGAATTGATTGGCCTGGTGGTGGTGTTATT
CCAACCGATGAACCTCAATGTGGTTTTTTGAATGAACAATGCATCAAAGATGATCGACAT
GTCATTTCGATGATAATATCTGGCATTTTGGGTCTTTTATTGTTTTGTTCAATTGTAATA
ACCATTAGTATTTATCGTAAATGGAAGATTGAGTTAGAAATTGAAGGTTTATTATGGAAA
ATTGAACCACATGAGATCAAGGGTTATTTTAATAATGATATTGTATCATCACCAAGCAAA
TTGAGTCTCGTGAGTGCAGCAAGTTACGGATCACGTTACCAAGTTTGGACAACAACGGGT
CAATTTCGTGGTGTTATTGTGAGAGTAAAAGAACTTAAGTTTGCCAAGAAAAAAGACATG
TCACGAGAGTTAATGAAAGAATTTCGAACTCTTCGTGATTTGCGTCATGATAATATTAAT
AGTTTTATAGGAGCGTGTGTTGAACCCATGCGAATTTTGCTAGTCACTGATTATTGTGCT
AAGGGAAGTTTATACGATATAATAGAAAATGAGGACATAAAATTAGATAATTTGTTTATT
GCATCGCTGATTAATGATTTAATTAAGGGTATGATATACATCCACTCATCAGCATTAGTA
TTTCATGGTAATCTCAAGTCATCAAATTGTGTCATAACATCGCGTTGGATGCTGCAAATA
ACGGATTTCGGTCTGCATGATTTACGGCATTCTGCAGAGAGTGGCGGCTCTGTTGGTGAA
CATCAATATTTCCGAAGTTTATTTTGGAAATCACCAGAGCTTCTTCGAAATCCAAATATT
CATGGCAGTCAGAAGGGTGATGTCTACGCATTTGCCATAATTCTTTACGAAATTTGCGGT
AGAAAGGGACCATTTGGTCTCGCCCACTATGAACCAAAGCAAATAATTGAATTAGTCAAA
GAAACGCCAATTGACGATGAAAAGCCACCATTTAGACCAGATTTAGAATGTCTCCTTGAA
GACGACAATTGTCCTGATTACGTAATCAGTTGCATTCAAGACTGCTGGCATGAAAATCCT
GAAGTTAGACCTGATTTTCCAACTATACGTACACGTCTGAAAAAGATGCGTGATGGCAAA
TCAAAGAACATTATGGATCAAATTATGGAAATGATGGAAAAATATACAAATAATTTGGAA
GATATTGTTAATGAAAGGACGAGATTATTGTATGAAGAAAAAAGAAAGACAGAAGACCTT
CTTAATCGAATGCTTCCACAACCAGTTGCTGAGAAATTAACTATGGGTTTAGGTGTTGAA
CCAGTCACTTATGATTCTGTTACAATATACTTTAGCGATATTGTTGGATTTACACAATTG
TCAGCTGAAAGCACACCACTACAAGTTGTACAATTTCTTAATGATCTATATACAGTTTTT
GACAGAATTATAAAAGGCTTTGATGTTTATAAAGTTGAAACTATTGGTGATGCTTACATG
GTAGTTTCTGGTTTGCCAATAACAAACGGAAATCGTCATGTTGGTGAAATTGCATCAATG
GCTCTAGATCTGCTTAAGGCTGTAAAAAATCATCATATATCTCACCGACCGAATGAAATA
CTTAAATTAAGAATTGGAATTCACACTGGGCCAGTTGTAGCTGGTGTAGTTGGACTAACA
ATGCCTAGATTTTGTCTATTTGGCGATACAGTTAATACGGCATCTCGTATGGAATCATGC
GGTGAGCCATTGAAAATTCACATATCGCAACAATGCAAGAATGCATTAGACAAATTAGGA
GGCTATGTAACTGAATGCCGAGGTGTTGTGCCGATGAAAGGAAAAGGCGATGTTTTAACT
TATTGGCTTACGGGAGCAACAGATGGTGCCATCCAAAAAAGAAATGTAGATTTAAGGGAC
TTACCTCCACCGTTATTTTGTCGACCGCGTAAAAGTCCAAAATGTTTGGGCGAATCAAAA
CAGCCATCGATCTCGTGTATTATGAACTTTGGCGCATCAGATAATCGAAGTATATTATGT
GAAAGTAGACGTCAATCAAATGCAGCAGCCCAAAGAAATGATGATGGTGGTGGCTCATAT
CGCGAATACAGAGATTCATCGTCACCGTTCATTTCAAAGCATCGAAAATATGATAACACT
CCATTATATATAAACAACAATGACTCAGAAATGATTACAGATACACTAGAAGATCTTAGA
AGAGACACTTTAAATGGAAGTGGTGAAAAGCCATATGCATTGATTCGACCACAACAGATC
AGTACAATTAATTCATCTAATGATGATATATGTTCATTGAGGCCATTTGATGAGGAACAT
TTAAGCTTTAAAAAGAGTCATTCATATGATCCATTTCCAGTTGCTGATCATCATCATCAT
AATCAACAATTAAATAAAAGAAATAATGAAAATTTCAGAAAGCGTAAGAACAACACGAGT
GAACCACTTAAAGAAGTTGGTTGCAATGGTGGCATAACATCACAAACTCAAACACAAAGA
TCTGACCACGAACCACTTCTTGAGCCAAAACGTTGGCATTCTTTAGAATTAGAGACAGTG
AGAACAAATCGAGATGAAGAAAGTAGTGGCTGTGAAGAACGTAATGGTACTAATAAAAAG
TATAATCGTATTAAATCGTGGCTAGTGGGAATTTTGAAAAGTAAAAATCCGAATGGACAA
AATGAGCTGTTGTCACAACAAACATCAATTACAAAAACGCGGAATGTTGAAAATGAGAGC
ATGAGTGTTGTGTGA
>g5319.t1 Gene=g5319 Length=1384
MSIIRAFIVTLVAFATIFNFVHGSHHIKTTLNVGYLTAIKGDLKDRQGLAISGAIQMALE
EINNDPKILPNVKLELWWNDTRGDTVIATRAITEMICNGVSTIFGPEGTCHVEAIVTQSR
NIPMIAYKCSDYKASAIPTFARTEPPDTQVTKSVIALLNYYRWNKFSIIYEEAWERVAES
LSQQAKSHNKTINHKKQVVDRHKCCEQNMDCCRPGYWYNIIQNTMNRTRIYIFLGTPDAL
VDMMGSMESIQLFDKGEYMVIFVDMMSYSAEEAKKYLYKLENFSKYKSCNEIEGFTKRAR
SLLVVVSTPPTKNYEEFTDKVRKYNEKKPFEFRNPSFPNRKNFVKFVSIYAAYLYDAVKL
YANALHKLLKKEELSRPLTDEVIREIASNGTAIISAIIQEKKYPSITGAEIWIDENGDSE
GNFSVLALKESQFSPKDSNFTCDRHMITVATFQGRNNNNSLPEYKFNNILRIDWPGGGVI
PTDEPQCGFLNEQCIKDDRHVISMIISGILGLLLFCSIVITISIYRKWKIELEIEGLLWK
IEPHEIKGYFNNDIVSSPSKLSLVSAASYGSRYQVWTTTGQFRGVIVRVKELKFAKKKDM
SRELMKEFRTLRDLRHDNINSFIGACVEPMRILLVTDYCAKGSLYDIIENEDIKLDNLFI
ASLINDLIKGMIYIHSSALVFHGNLKSSNCVITSRWMLQITDFGLHDLRHSAESGGSVGE
HQYFRSLFWKSPELLRNPNIHGSQKGDVYAFAIILYEICGRKGPFGLAHYEPKQIIELVK
ETPIDDEKPPFRPDLECLLEDDNCPDYVISCIQDCWHENPEVRPDFPTIRTRLKKMRDGK
SKNIMDQIMEMMEKYTNNLEDIVNERTRLLYEEKRKTEDLLNRMLPQPVAEKLTMGLGVE
PVTYDSVTIYFSDIVGFTQLSAESTPLQVVQFLNDLYTVFDRIIKGFDVYKVETIGDAYM
VVSGLPITNGNRHVGEIASMALDLLKAVKNHHISHRPNEILKLRIGIHTGPVVAGVVGLT
MPRFCLFGDTVNTASRMESCGEPLKIHISQQCKNALDKLGGYVTECRGVVPMKGKGDVLT
YWLTGATDGAIQKRNVDLRDLPPPLFCRPRKSPKCLGESKQPSISCIMNFGASDNRSILC
ESRRQSNAAAQRNDDGGGSYREYRDSSSPFISKHRKYDNTPLYINNNDSEMITDTLEDLR
RDTLNGSGEKPYALIRPQQISTINSSNDDICSLRPFDEEHLSFKKSHSYDPFPVADHHHH
NQQLNKRNNENFRKRKNNTSEPLKEVGCNGGITSQTQTQRSDHEPLLEPKRWHSLELETV
RTNRDEESSGCEERNGTNKKYNRIKSWLVGILKSKNPNGQNELLSQQTSITKTRNVENES
MSVV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 22 | g5319.t1 | CDD | cd06370 | PBP1_SAP_GC-like | 31 | 452 | 1.47596E-145 |
| 23 | g5319.t1 | CDD | cd14042 | PK_GC-A_B | 554 | 839 | 3.4279E-136 |
| 24 | g5319.t1 | CDD | cd07302 | CHD | 907 | 1083 | 1.79368E-66 |
| 14 | g5319.t1 | Coils | Coil | Coil | 845 | 872 | - |
| 13 | g5319.t1 | Gene3D | G3DSA:3.40.50.2300 | - | 51 | 420 | 1.6E-70 |
| 12 | g5319.t1 | Gene3D | G3DSA:3.40.50.2300 | - | 146 | 489 | 1.6E-70 |
| 11 | g5319.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 552 | 871 | 3.9E-61 |
| 10 | g5319.t1 | Gene3D | G3DSA:3.30.70.1230 | Adenylyl Cyclase | 900 | 1087 | 3.1E-73 |
| 30 | g5319.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1143 | 1167 | - |
| 31 | g5319.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1257 | 1337 | - |
| 33 | g5319.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1257 | 1281 | - |
| 32 | g5319.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1303 | 1337 | - |
| 4 | g5319.t1 | PANTHER | PTHR11920 | GUANYLYL CYCLASE | 24 | 1284 | 0.0 |
| 5 | g5319.t1 | PANTHER | PTHR11920:SF474 | RECEPTOR-TYPE GUANYLATE CYCLASE GYC76C | 24 | 1284 | 0.0 |
| 2 | g5319.t1 | Pfam | PF01094 | Receptor family ligand binding region | 52 | 431 | 7.8E-43 |
| 3 | g5319.t1 | Pfam | PF07714 | Protein tyrosine and serine/threonine kinase | 579 | 833 | 4.3E-37 |
| 1 | g5319.t1 | Pfam | PF00211 | Adenylate and Guanylate cyclase catalytic domain | 903 | 1084 | 3.8E-64 |
| 17 | g5319.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 23 | - |
| 18 | g5319.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 19 | g5319.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 18 | - |
| 21 | g5319.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 19 | 23 | - |
| 16 | g5319.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 24 | 500 | - |
| 20 | g5319.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 501 | 525 | - |
| 15 | g5319.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 526 | 1384 | - |
| 29 | g5319.t1 | ProSitePatterns | PS00452 | Guanylate cyclase signature. | 1015 | 1038 | - |
| 35 | g5319.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 558 | 833 | 29.715 |
| 34 | g5319.t1 | ProSiteProfiles | PS50125 | Guanylate cyclase domain profile. | 908 | 1038 | 51.477 |
| 28 | g5319.t1 | SMART | SM00220 | serkin_6 | 558 | 834 | 1.8E-14 |
| 27 | g5319.t1 | SMART | SM00044 | cyc_6 | 872 | 1066 | 1.4E-98 |
| 6 | g5319.t1 | SUPERFAMILY | SSF53822 | Periplasmic binding protein-like I | 30 | 489 | 2.88E-73 |
| 7 | g5319.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 573 | 878 | 3.99E-51 |
| 8 | g5319.t1 | SUPERFAMILY | SSF55073 | Nucleotide cyclase | 901 | 1088 | 7.69E-63 |
| 9 | g5319.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 23 | - |
| 26 | g5319.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 4 | 23 | - |
| 25 | g5319.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 501 | 523 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5319/g5319.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5319.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016849 | phosphorus-oxygen lyase activity | MF |
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0009190 | cyclic nucleotide biosynthetic process | BP |
| GO:0006468 | protein phosphorylation | BP |
| GO:0035556 | intracellular signal transduction | BP |
KEGG
Orthology
- This transcript has been annotated with K01769 (guanylate cyclase, other)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
