General information for g5319.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Receptor-type guanylate cyclase Gyc76C.
Parent gene
- The parent gene g5319 is located on chr_2: 8733442..8733905 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5319 | g5319.t2 | isoform | g5319.t2 | 8732738 | 8733905 |
| chr_2 | g5319 | g5319.t2 | exon | g5319.t2.exon1 | 8732738 | 8733385 |
| chr_2 | g5319 | g5319.t2 | cds | g5319.t2.CDS1 | 8732755 | 8733385 |
| chr_2 | g5319 | g5319.t2 | exon | g5319.t2.exon2 | 8733442 | 8733905 |
| chr_2 | g5319 | g5319.t2 | cds | g5319.t2.CDS2 | 8733442 | 8733905 |
| chr_2 | g5319 | g5319.t2 | TTS | g5319.t2 | 8733990 | 8733990 |
| chr_2 | g5319 | g5319.t2 | TSS | g5319.t2 | NA | NA |
Sequences
>g5319.t2 Gene=g5319 Length=1112
GTGTAGTTGGACTAACAATGCCTAGATTTTGTCTATTTGGCGATACAGTTAATACGGCAT
CTCGTATGGAATCATGCGGTGAGCCATTGAAAATTCACATATCGCAACAATGCAAGAATG
CATTAGACAAATTAGGAGGCTATGTAACTGAATGCCGAGGTGTTGTGCCGATGAAAGGAA
AAGGCGATGTTTTAACTTATTGGCTTACGGGAGCAACAGATGGTGCCATCCAAAAAAGAA
ATGTAGATTTAAGGGACTTACCTCCACCGTTATTTTGTCGACCGCGTAAAAGTCCAAAAT
GTTTGGGCGAATCAAAACAGCCATCGATCTCGTGTATTATGAACTTTGGCGCATCAGATA
ATCGAAGTATATTATGTGAAAGTAGACGTCAATCAAATGCAGCAGCCCAAAGAAATGATG
ATGGTGGTGGCTCATATCGCGAATACAGAGATTCATCGTCACCGTTCATTTCAAAGCATC
GAAAATATGATAACACTCCATTATATATAAACAACAATGACTCAGAAATGATTACAGATA
CACTAGAAGATCTTAGAAGAGACACTTTAAATGGAAGTGGTGAAAAGCCATATGCATTGA
TTCGACCACAACAGATCAGTACAATTAATTCATCTAATGATGATATATGTTCATTGAGGC
CATTTGATGAGGAACATTTAAGCTTTAAAAAGAGTCATTCATATGATCCATTTCCAGTTG
CTGATCATCATCATCATAATCAACAATTAAATAAAAGAAATAATGAAAATTTCAGAAAGC
GTAAGAACAACACGAGTGAACCACTTAAAGAAGTTGGTTGCAATGGTGGCATAACATCAC
AAACTCAAACACAAAGATCTGACCACGAACCACTTCTTGAGCCAAAACGTTGGCATTCTT
TAGAATTAGAGACAGTGAGAACAAATCGAGATGAAGAAAGTAGTGGCTGTGAAGAACGTA
ATGGTACTAATAAAAAGTATAATCGTATTAAATCGTGGCTAGTGGGAATTTTGAAAAGTA
AAAATCCGAATGGACAAAATGAGCTGTTGTCACAACAAACATCAATTACAAAAACGCGGA
ATGTTGAAAATGAGAGCATGAGTGTTGTGTGA
>g5319.t2 Gene=g5319 Length=364
MPRFCLFGDTVNTASRMESCGEPLKIHISQQCKNALDKLGGYVTECRGVVPMKGKGDVLT
YWLTGATDGAIQKRNVDLRDLPPPLFCRPRKSPKCLGESKQPSISCIMNFGASDNRSILC
ESRRQSNAAAQRNDDGGGSYREYRDSSSPFISKHRKYDNTPLYINNNDSEMITDTLEDLR
RDTLNGSGEKPYALIRPQQISTINSSNDDICSLRPFDEEHLSFKKSHSYDPFPVADHHHH
NQQLNKRNNENFRKRKNNTSEPLKEVGCNGGITSQTQTQRSDHEPLLEPKRWHSLELETV
RTNRDEESSGCEERNGTNKKYNRIKSWLVGILKSKNPNGQNELLSQQTSITKTRNVENES
MSVV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g5319.t2 | CDD | cd07302 | CHD | 1 | 63 | 8.73921E-17 |
| 8 | g5319.t2 | Gene3D | G3DSA:3.30.70.1230 | Adenylyl Cyclase | 1 | 67 | 8.8E-22 |
| 7 | g5319.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 123 | 147 | - |
| 5 | g5319.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 237 | 261 | - |
| 6 | g5319.t2 | MobiDBLite | mobidb-lite | consensus disorder prediction | 237 | 294 | - |
| 2 | g5319.t2 | PANTHER | PTHR11920 | GUANYLYL CYCLASE | 1 | 269 | 5.6E-49 |
| 3 | g5319.t2 | PANTHER | PTHR11920:SF474 | RECEPTOR-TYPE GUANYLATE CYCLASE GYC76C | 1 | 269 | 5.6E-49 |
| 1 | g5319.t2 | Pfam | PF00211 | Adenylate and Guanylate cyclase catalytic domain | 1 | 64 | 7.7E-17 |
| 9 | g5319.t2 | ProSiteProfiles | PS50125 | Guanylate cyclase domain profile. | 1 | 18 | 10.914 |
| 4 | g5319.t2 | SUPERFAMILY | SSF55073 | Nucleotide cyclase | 1 | 68 | 1.4E-19 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5319/g5319.t2; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5319.t2.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0009190 | cyclic nucleotide biosynthetic process | BP |
| GO:0035556 | intracellular signal transduction | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
