General information for g5335.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Plasmanylethanolamine desaturase.
Parent gene
- The parent gene g5335 is located on chr_2: 8882656..8886101 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5335 | g5335.t2 | isoform | g5335.t2 | 8880784 | 8886101 |
| chr_2 | g5335 | g5335.t2 | TSS | g5335.t2 | 8880784 | 8880784 |
| chr_2 | g5335 | g5335.t2 | exon | g5335.t2.exon1 | 8880784 | 8880943 |
| chr_2 | g5335 | g5335.t2 | exon | g5335.t2.exon2 | 8882646 | 8882766 |
| chr_2 | g5335 | g5335.t2 | cds | g5335.t2.CDS1 | 8882656 | 8882766 |
| chr_2 | g5335 | g5335.t2 | exon | g5335.t2.exon3 | 8882959 | 8883115 |
| chr_2 | g5335 | g5335.t2 | cds | g5335.t2.CDS2 | 8882959 | 8883115 |
| chr_2 | g5335 | g5335.t2 | exon | g5335.t2.exon4 | 8885349 | 8886101 |
| chr_2 | g5335 | g5335.t2 | cds | g5335.t2.CDS3 | 8885349 | 8885953 |
| chr_2 | g5335 | g5335.t2 | TTS | g5335.t2 | 8886322 | 8886322 |
Sequences
>g5335.t2 Gene=g5335 Length=1191
CAGTTTAGTTTTGTCATTTGTCGAAAAAAGGTTGAAAAAAGTGAATTCTCCTTTGAATTA
AAAAGAACCGGCAATAAAAAAAGATAAAGTCAAAAAAAATTATGCTCTATCAATCATAAT
TATTGCAATCAATTTCAAAGTTCGTGATAAGAAATTAAAAAATAAAAAATATGGATATCA
TAAGCATTTCTCCCTGCATAAAGCGAACATCAGAAGTGGGTAAAGAAGATTGTCCACACA
TTCCAAGCGTCTCGAAAAGTGAAATTCAAAATTTTCTCACGCCAAGACCAGTAAAAACTG
ATCAAGAAATTATTGAAAGTTCCATGGCTGAAGATGATCCAAATTGCATATCAGAAGATG
AAGACACAACCTTTGAAAAAGTTCGCTGGGGTAGACATCATAAAGGAGCAAAAATTCTTG
CAAATTTTTATAGCAGTGGAAAACGAACACAGGAAATAATCTGCGTCTACTCTTGCATTT
CACTTATGTTTATCAATTTTTATTTTATTCTAAGTCATTTTCGTATGGATTATTTCTCAA
AAGTTGTGCTATCAGGAGTATGTGGAATTTTAACAGCTGATTTTGGTAGTGGTCTTGTTC
ATTGGGGCGCAGATACTTGGGGAAGCATTGAATTGCCGATTATTGGAAAAAACTTTCTTC
GACCTTTTCGTGAACATCACATCGACCCAACGAGCATCACGCGACATGATTGGATTGAGA
CTAATGGTGATAATTTTATGATTGCAATCCCAATTTTATCAAGACTGACTTGGATTTTTT
ATATGACACCCAAGTCTGAAATTCCTGAAGTTTATCCCTTTTGTGCATTCTTATTTCTTT
GTTCAATTTTTGTTGCAATTACAAATCAAATTCACAAATGGTCGCATACTTACTTTGACC
TGCCATTTTGGGTACAAATTCTACAAAACTATCATATCATACTGCCACGAAAACATCATC
GAATTCATCATGTTGCACCTCATGAAACTTACTTTTGTATTACAACTGGGTGGTTAAATT
ATCCTTTAGAAAAAATCAGGTAAAATCTTCAAGAAAATTTCCTAAATTCTTTGATTGATA
AAAAATATTTTTAAAATTAGATTCTGGCCAACATTAGAATCAATTGTGACATTAATAACA
GGCTATAAACCACGTGAAGATGATCTCAAATGGGCATCAAAGACATCGTAA
>g5335.t2 Gene=g5335 Length=290
MDIISISPCIKRTSEVGKEDCPHIPSVSKSEIQNFLTPRPVKTDQEIIESSMAEDDPNCI
SEDEDTTFEKVRWGRHHKGAKILANFYSSGKRTQEIICVYSCISLMFINFYFILSHFRMD
YFSKVVLSGVCGILTADFGSGLVHWGADTWGSIELPIIGKNFLRPFREHHIDPTSITRHD
WIETNGDNFMIAIPILSRLTWIFYMTPKSEIPEVYPFCAFLFLCSIFVAITNQIHKWSHT
YFDLPFWVQILQNYHIILPRKHHRIHHVAPHETYFCITTGWLNYPLEKIR
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 2 | g5335.t2 | PANTHER | PTHR48177 | TRANSMEMBRANE PROTEIN 189 | 63 | 289 | 1.4E-95 |
| 1 | g5335.t2 | Pfam | PF10520 | B domain of TMEM189, localisation domain | 133 | 289 | 3.1E-65 |
| 6 | g5335.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 95 | - |
| 10 | g5335.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 96 | 114 | - |
| 8 | g5335.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 115 | 213 | - |
| 9 | g5335.t2 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 214 | 231 | - |
| 7 | g5335.t2 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 232 | 290 | - |
| 4 | g5335.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 96 | 118 | - |
| 3 | g5335.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 125 | 147 | - |
| 5 | g5335.t2 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 211 | 230 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5335/g5335.t2; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5335.t2.fa.iupred3.txt does not exist
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed