General information for g5385.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Engulfment and cell motility protein 1.
Parent gene
- The parent gene g5385 is located on chr_2: 9310559..9312833 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5385 | g5385.t2 | TSS | g5385.t2 | 9310311 | 9310311 |
| chr_2 | g5385 | g5385.t2 | isoform | g5385.t2 | 9310559 | 9311524 |
| chr_2 | g5385 | g5385.t2 | exon | g5385.t2.exon1 | 9310559 | 9311524 |
| chr_2 | g5385 | g5385.t2 | cds | g5385.t2.CDS1 | 9310559 | 9311524 |
| chr_2 | g5385 | g5385.t2 | TTS | g5385.t2 | NA | NA |
Sequences
>g5385.t2 Gene=g5385 Length=966
ATGTTACCATCAAGAATGCCTGCTGTTCGTGATGCATCGATTGTAAAAATAGCTGTAGAG
GCACTAGCCGCTGGCTTTTTGCCTCAACTTATTGAATTCGATCAGCGGCAACCACTAACT
AGTATAATAAATGATATTTGTAATTCTTGGAATATTGAAGATTCACATAATTATTCCTTA
AAATTTACTGAACAAAGCAACAAAGGCTATGTGACAGAGAAAAATCGATGTGGTGTTAAA
AATGGAACTGTATTGAAATTAAATTACAGTGCAACCAAAACAGTCACAGATTTTCTCGAA
ATTTTTCGTAATGGATCCGTTATTGAGCAAGCAAAATGTTTACAGAAATTGGCTAGTCTT
TCTTCTGATATCACTTTTGCGATTGAATTTATCAATAAATCTGGCCTAGATTTGCTCATT
AAAATGATTGAAGACGAAAAATGTATTGGTGAACGGCTTGAGTATGCACTTCGTTCATTT
GGAGAATTGATGGATCATGGAACAGTATCGTGGGAAATTTTAAGTGATGCATTTGTTAAT
CGTAATATTGCTGCGATATCTAATTCAAGTAATTCACCACATCCCATTCCTCCACATCTC
ATTGCTTCATCTTTGTCAATTCTTGAAAATGTCGTTCAAAATAGTAGCCGTTATGCTCTT
GTTGAACGTTCTGTGACTTTTGATACTCTTTTGAAACTTCTTCGCGAACAAAATCCAAAT
ATTCAACAAAATACCATTGCTCTCATAAATGCACTTTTTATGCGTGCAGATGAAGCTAAA
AGAAAAATAATTGCAAGCACATTTAGCACAAAGCAATATAGAAGTACTCTACTTGATAGT
TGTGTATTGAATGCTCAAATTGGAAAAGAAATGACTCATCAATTAAGCGTCTTACAATCA
TTGACACTTGGTATGCTGGAACCAAGAATGAATGACAGAAATGTTGATGCAGATGCACAA
GAGAAA
>g5385.t2 Gene=g5385 Length=322
MLPSRMPAVRDASIVKIAVEALAAGFLPQLIEFDQRQPLTSIINDICNSWNIEDSHNYSL
KFTEQSNKGYVTEKNRCGVKNGTVLKLNYSATKTVTDFLEIFRNGSVIEQAKCLQKLASL
SSDITFAIEFINKSGLDLLIKMIEDEKCIGERLEYALRSFGELMDHGTVSWEILSDAFVN
RNIAAISNSSNSPHPIPPHLIASSLSILENVVQNSSRYALVERSVTFDTLLKLLREQNPN
IQQNTIALINALFMRADEAKRKIIASTFSTKQYRSTLLDSCVLNAQIGKEMTHQLSVLQS
LTLGMLEPRMNDRNVDADAQEK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g5385.t2 | Gene3D | G3DSA:1.25.10.10 | - | 85 | 283 | 0 |
| 2 | g5385.t2 | PANTHER | PTHR12771:SF16 | ENGULFMENT AND CELL MOTILITY PROTEIN 3 | 12 | 321 | 0 |
| 3 | g5385.t2 | PANTHER | PTHR12771 | ENGULFMENT AND CELL MOTILITY | 12 | 321 | 0 |
| 1 | g5385.t2 | Pfam | PF11841 | Domain of unknown function (DUF3361) | 125 | 280 | 0 |
| 4 | g5385.t2 | SUPERFAMILY | SSF48371 | ARM repeat | 97 | 264 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5385/g5385.t2; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5385.t2.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016477 | cell migration | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed