General information for g5396.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as UDP-glucuronosyltransferase 2B7.
Parent gene
- The parent gene g5396 is located on chr_2: 9391628..9393337 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5396 | g5396.t1 | TSS | g5396.t1 | 9391396 | 9391396 |
| chr_2 | g5396 | g5396.t1 | isoform | g5396.t1 | 9391628 | 9393337 |
| chr_2 | g5396 | g5396.t1 | exon | g5396.t1.exon1 | 9391628 | 9391630 |
| chr_2 | g5396 | g5396.t1 | cds | g5396.t1.CDS1 | 9391628 | 9391630 |
| chr_2 | g5396 | g5396.t1 | exon | g5396.t1.exon2 | 9391693 | 9391821 |
| chr_2 | g5396 | g5396.t1 | cds | g5396.t1.CDS2 | 9391693 | 9391821 |
| chr_2 | g5396 | g5396.t1 | exon | g5396.t1.exon3 | 9391877 | 9393337 |
| chr_2 | g5396 | g5396.t1 | cds | g5396.t1.CDS3 | 9391877 | 9393337 |
| chr_2 | g5396 | g5396.t1 | TTS | g5396.t1 | 9393517 | 9393517 |
Sequences
>g5396.t1 Gene=g5396 Length=1593
ATGCAGTGTAAATTGTTTGTTGTCATTGCACTTGCCGCATGTCTGAGCTTCACTACGTCA
TCGAAAATTCTTTTTCTTGTTGCATTCCCCGGTAAAAGTCATTGGTTGATGTTTGAACCT
GTTATCAATGAACTTCTAAATCGAGGCCATGAGGTCACTGCAATTACTCACTATCCATTG
AAAACTGATTCTAAAAATTATCATGAAATTCTCATTAATCCATCATGGATATGGGAAGAT
AGAGTCAATATGACAAGTTATTTTACTAGAAGTGTCATTAAAGAAAGTGTAATTTTTAAA
ATCACTTCACTTTGGGACTTTGGAATAGTTACAACAAATTTTACATTAAATTCACCGCCT
ATCAAGAAATTCATTGAAGATGACAATCAAAAGTTTGATTTGGTCATATCGGAGCAATTT
CAGCAGGAAGCAATGAATATGTTTGCGTACAAATACAATTGCCCGATAGTTGCTATTGGA
ACACTCGATTATGCCGACTATATGCATCATGCAAAGGGTCAAATTACTCCATGGTCATAT
GTGCCTCATTTTCTTAGTTATAGCACAGATCAAATGACATTGATTGAACGACTCGAAAAT
TTTTTCGTATCACTTTACGATGCAATTGGCAGGAAATTTTATTATCTACCAAAAATGACA
AATATGGCAAGAGAAGCATTCAAAAAGCTTGAAAATCAACAAGGTCGTTTGCCATCGGTT
GAAAATTTAGAGAAAAAAATTGCTGTACATTTGATGAATTCACATCCGGCACTTTCATAT
CCTCGTCCTAAAATGCCAGGAATGATTGACATTGCTGGAATTCATATAAAAGAACAAAAG
CCATTGCCAAAAGATATTCAAAAATTTATGGATGATGCAAATGATGGAGTAATTTTAATT
AGTTTCGGAACATTCTTACAAAGTTCTAAAATGCCACCAGCACAATATAAAGCAATGCTT
GATGCATTTAGTAAACTTAAACAGAGAATAATTTGGAAATGGGAAGAAGACAATAAAAAT
GTTAAATTTCCATCAAATGTTATGGTACGAAAATGGCTACCACAAGCTGATATTTTAGCA
CATAAGAATTGTAAGCTTATGATCGGACATGGAGGAATCTTTGGCACACAAGAAGCAATT
TATTATGGAAAACCGATGATTGTTTTTCCATTTTATGGTGATCAACATCTCAATGGCTAC
AAACTTGAACGTGATGGTATAGGAATATTACAGGCCATTAATGAATTGACTTCAGATTCA
TTACTTGCTGCCATAAATCGTATTCTGAATGATGCAACATTTTATGAGAATGTAAAGAGA
AAATCAGAAATTTTCAGAGCTAATCAAAATCCTCCACTTGAGACTGCAATATTTTGGATT
GAATATGTTCTAAATTTTAATGGTGCAGAACATTTGCAATCACATGGAAAGAATTTATCA
TGGATAAGAATTTTGTCAATTGATATTATGCTATTGATATTTGGAGGAATTTATTTGATT
TATGACTTGATAAAAATGATTGCTGGAAGAAAGATTAAAGATGATACTAACAAGACTGAT
AAGAAAAAGAAGAACAAAAAGAAGGAAAACTAA
>g5396.t1 Gene=g5396 Length=530
MQCKLFVVIALAACLSFTTSSKILFLVAFPGKSHWLMFEPVINELLNRGHEVTAITHYPL
KTDSKNYHEILINPSWIWEDRVNMTSYFTRSVIKESVIFKITSLWDFGIVTTNFTLNSPP
IKKFIEDDNQKFDLVISEQFQQEAMNMFAYKYNCPIVAIGTLDYADYMHHAKGQITPWSY
VPHFLSYSTDQMTLIERLENFFVSLYDAIGRKFYYLPKMTNMAREAFKKLENQQGRLPSV
ENLEKKIAVHLMNSHPALSYPRPKMPGMIDIAGIHIKEQKPLPKDIQKFMDDANDGVILI
SFGTFLQSSKMPPAQYKAMLDAFSKLKQRIIWKWEEDNKNVKFPSNVMVRKWLPQADILA
HKNCKLMIGHGGIFGTQEAIYYGKPMIVFPFYGDQHLNGYKLERDGIGILQAINELTSDS
LLAAINRILNDATFYENVKRKSEIFRANQNPPLETAIFWIEYVLNFNGAEHLQSHGKNLS
WIRILSIDIMLLIFGGIYLIYDLIKMIAGRKIKDDTNKTDKKKKNKKKEN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g5396.t1 | CDD | cd03784 | GT1_Gtf-like | 22 | 448 | 7.99599E-64 |
| 6 | g5396.t1 | Gene3D | G3DSA:3.40.50.2000 | Glycogen Phosphorylase B; | 277 | 447 | 1.1E-46 |
| 2 | g5396.t1 | PANTHER | PTHR48043 | EG:EG0003.4 PROTEIN-RELATED | 14 | 499 | 9.5E-183 |
| 3 | g5396.t1 | PANTHER | PTHR48043:SF59 | UDP-GLUCURONOSYLTRANSFERASE | 14 | 499 | 9.5E-183 |
| 1 | g5396.t1 | Pfam | PF00201 | UDP-glucoronosyl and UDP-glucosyl transferase | 31 | 491 | 6.4E-75 |
| 9 | g5396.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 21 | - |
| 10 | g5396.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 11 | g5396.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 16 | - |
| 13 | g5396.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 21 | - |
| 8 | g5396.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 22 | 480 | - |
| 12 | g5396.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 481 | 501 | - |
| 7 | g5396.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 502 | 530 | - |
| 18 | g5396.t1 | ProSitePatterns | PS00375 | UDP-glycosyltransferases signature. | 352 | 395 | - |
| 4 | g5396.t1 | SUPERFAMILY | SSF53756 | UDP-Glycosyltransferase/glycogen phosphorylase | 22 | 472 | 1.77E-99 |
| 15 | g5396.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 21 | - |
| 5 | g5396.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 23 | - |
| 16 | g5396.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 7 | 29 | - |
| 17 | g5396.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 479 | 501 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5396/g5396.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5396.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008194 | UDP-glycosyltransferase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K00699 (glucuronosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
