General information for g5489.t32
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Threonine–tRNA ligase 1, cytoplasmic.
Parent gene
- The parent gene g5489 is located on chr_2: 9846707..9847795 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5489 | g5489.t32 | isoform | g5489.t32 | 9845626 | 9847310 |
| chr_2 | g5489 | g5489.t32 | exon | g5489.t32.exon1 | 9845626 | 9846716 |
| chr_2 | g5489 | g5489.t32 | cds | g5489.t32.CDS1 | 9845626 | 9846716 |
| chr_2 | g5489 | g5489.t32 | exon | g5489.t32.exon2 | 9846810 | 9846867 |
| chr_2 | g5489 | g5489.t32 | cds | g5489.t32.CDS2 | 9846810 | 9846825 |
| chr_2 | g5489 | g5489.t32 | exon | g5489.t32.exon3 | 9847244 | 9847310 |
| chr_2 | g5489 | g5489.t32 | TSS | g5489.t32 | 9847335 | 9847335 |
| chr_2 | g5489 | g5489.t32 | TTS | g5489.t32 | NA | NA |
Sequences
>g5489.t32 Gene=g5489 Length=1216
ATGAGCAGTGAAATTTCGGAAAAATTAGAAAATTTGGATATAGAATCGAATCAGGCATGA
GATAATAGCCGCAAAACAAAAGGCTGCGAAAAAGAAGAAACAGGAAGAAATGGGTGTTGA
AACAGGAATTAAAGAATTGTCTCCACCACCATCATACATTGATGAGCGTATCAAACTATA
TGATCGTTTAAAAGCAGAATATCTTGAAGAATTGAAAGCTAAACCGAGAGAAAAAATTCA
AATTACATTGCCTGATGGAAAGATTGTTGAAGGATTATCATGGGAAACAACACCTTTTCA
AATTGCTGCAGGTATCAGTCAAGGATTAGCAGATAATACTGTTGTTGCAAAAGTAGATGA
TGAAGTATGGGATTTAGATCGTGTACTTGAGAAAAGTTGCGCATTGAAACTTTTAAAATT
TGATGATCCTGAAGCACAAGCTGTTTTTTGGCATAGCTCAGCTCATATTCTTGGTGAAGC
AATGGAAAGATGTTATGGTGGTTGTTTATGTTATGGACCTCCAATTGAAAATGGCTTTTA
TTATGACATGTTTTTGGATGGAGAAAATGTTTCTCCAACTCATTACAAAACACTCGAGGA
TCTCATTAAAAAAATTGTCAAAGAAAAGCAGCCATTTGAACGTTTGGAAATGAAAAAGTC
GGATTTATTAAAAATGTTTGAATACAATGAGTTTAAAAAGAGAATTCTCAATGAGAAAGT
TGACACTGAAACTACTACTGTTTACCGTTGCGGACCATTAATTGATTTGTGTCGAGGACC
TCATGTACGTCACACAGGCAAAATTAAGGCAATGAAAGTAACAAAAAGCTCATCAACATA
TTGGGAAGGAAAAGCTGATGCTGAAACTTTACAACGCATTTACGGAATTTCATTCCCTGA
TCCAAAACAATTGAAAGAATGGGAAAAGATTCAAGAAGAGGCAGCAAAACGTGATCATCG
TAAAATTGGACGAGAACAAGAACTCTTTTTCTTTCACGAATTATCACCAGGTTCATGCTT
TTTCCAACCTCGTGGTGCTCACATTTATAATACATTGATGAGCATGATTAGAGAGGAATA
TCGCAAGCGAGGTTTTCAAGAAGTTATTTCGCCAAACATGTATAATGCAAAGTTATGGCA
AACATCAGGACATTGGCAACATTATGCAGAAAATATGTTTTCGTTTGACGTTGAAAAGGA
AAAATATGCTCTTAAG
>g5489.t32 Gene=g5489 Length=369
MGVETGIKELSPPPSYIDERIKLYDRLKAEYLEELKAKPREKIQITLPDGKIVEGLSWET
TPFQIAAGISQGLADNTVVAKVDDEVWDLDRVLEKSCALKLLKFDDPEAQAVFWHSSAHI
LGEAMERCYGGCLCYGPPIENGFYYDMFLDGENVSPTHYKTLEDLIKKIVKEKQPFERLE
MKKSDLLKMFEYNEFKKRILNEKVDTETTTVYRCGPLIDLCRGPHVRHTGKIKAMKVTKS
SSTYWEGKADAETLQRIYGISFPDPKQLKEWEKIQEEAAKRDHRKIGREQELFFFHELSP
GSCFFQPRGAHIYNTLMSMIREEYRKRGFQEVISPNMYNAKLWQTSGHWQHYAENMFSFD
VEKEKYALK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g5489.t32 | CDD | cd01667 | TGS_ThrRS | 43 | 108 | 0.000 |
| 11 | g5489.t32 | Gene3D | G3DSA:3.10.20.30 | - | 41 | 102 | 0.000 |
| 9 | g5489.t32 | Gene3D | G3DSA:3.30.980.10 | - | 107 | 262 | 0.000 |
| 10 | g5489.t32 | Gene3D | G3DSA:3.30.930.10 | Bira Bifunctional Protein; Domain 2 | 264 | 369 | 0.000 |
| 3 | g5489.t32 | PANTHER | PTHR11451 | THREONINE-TRNA LIGASE | 5 | 369 | 0.000 |
| 4 | g5489.t32 | PANTHER | PTHR11451:SF54 | THREONYL-TRNA SYNTHETASE | 5 | 369 | 0.000 |
| 2 | g5489.t32 | Pfam | PF02824 | TGS domain | 43 | 103 | 0.000 |
| 1 | g5489.t32 | Pfam | PF07973 | Threonyl and Alanyl tRNA synthetase second additional domain | 210 | 258 | 0.000 |
| 13 | g5489.t32 | ProSiteProfiles | PS51880 | TGS domain profile. | 41 | 103 | 19.468 |
| 12 | g5489.t32 | ProSiteProfiles | PS50862 | Aminoacyl-transfer RNA synthetases class-II family profile. | 309 | 369 | 10.225 |
| 8 | g5489.t32 | SMART | SM00863 | tRNA_SAD_4 | 209 | 258 | 0.000 |
| 7 | g5489.t32 | SUPERFAMILY | SSF81271 | TGS-like | 43 | 103 | 0.000 |
| 6 | g5489.t32 | SUPERFAMILY | SSF55186 | ThrRS/AlaRS common domain | 105 | 281 | 0.000 |
| 5 | g5489.t32 | SUPERFAMILY | SSF55681 | Class II aaRS and biotin synthetases | 258 | 368 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5489/g5489.t32; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5489.t32.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004829 | threonine-tRNA ligase activity | MF |
| GO:0000166 | nucleotide binding | MF |
| GO:0043039 | tRNA aminoacylation | BP |
| GO:0004812 | aminoacyl-tRNA ligase activity | MF |
| GO:0006435 | threonyl-tRNA aminoacylation | BP |
| GO:0005737 | cytoplasm | CC |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
