General information for g5575.t4
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Connector enhancer of kinase suppressor of ras 2.
Parent gene
- The parent gene g5575 is located on chr_2: 10350059..10354617 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5575 | g5575.t4 | TSS | g5575.t4 | 10349808 | 10349808 |
| chr_2 | g5575 | g5575.t4 | isoform | g5575.t4 | 10350059 | 10351053 |
| chr_2 | g5575 | g5575.t4 | exon | g5575.t4.exon1 | 10350059 | 10350116 |
| chr_2 | g5575 | g5575.t4 | cds | g5575.t4.CDS1 | 10350059 | 10350116 |
| chr_2 | g5575 | g5575.t4 | exon | g5575.t4.exon2 | 10350178 | 10350341 |
| chr_2 | g5575 | g5575.t4 | cds | g5575.t4.CDS2 | 10350178 | 10350341 |
| chr_2 | g5575 | g5575.t4 | exon | g5575.t4.exon3 | 10350409 | 10350469 |
| chr_2 | g5575 | g5575.t4 | cds | g5575.t4.CDS3 | 10350409 | 10350469 |
| chr_2 | g5575 | g5575.t4 | exon | g5575.t4.exon4 | 10350528 | 10351053 |
| chr_2 | g5575 | g5575.t4 | cds | g5575.t4.CDS4 | 10350528 | 10351051 |
| chr_2 | g5575 | g5575.t4 | TTS | g5575.t4 | NA | NA |
Sequences
>g5575.t4 Gene=g5575 Length=809
ATGGCATATATTAATGTAAATGATTGGAAATGTGAGCAAGTTATTGAATGGTTAAAAGGA
ATTGAATGCGTAAATAATTTTTACATAAAAGCATTTGCGAATAATCAAATAGGAGGATTT
CAATTGCTCTATATCACACATACAGAACTAAATGAATTGGGTATAAAGAAGATTGGGCAT
CAAGAATTGATATTAGAGGCAGTTGAGCATTTGAAAAATTTTCATTTTAATCTTGAAAAG
GAAAATTTGCAATATCTAGCTCTTCAAGTAGCAACAACGTCCACATCATTATTTAAACAA
CTTTCATATAATCCAGACACAAAACTTGAAAGTGTTGTTTTAAAAGATGTTGCCTACATC
ATATCCAAAGTCAAAACTCTTATTAGTTGGCTCGATAATTCTCCTTTTAAAGGACATTCA
CAATATAGTGAAATGCGTAGAAATTGGCTAAAATTAGGTTTAGAACTCGCGACAATTGCT
CAAAGAGATAGATTTGTCCAAAATCCAATCAAACAAATTACTAGCATAACACAAACTTTA
TCAAAACTTTCTGACTATATTATACATGACATTACTGATCCAATGGTTTTGCAACCTGCT
TTTCTGGATTTGGTGACATTGAAGAAAAAAGAATCAGAGTTAGGCTTCTATATAATGCCT
AATTATCAATCTATTCATAGAGTAACAGATATTAAATATAATTCTCCTGCACATAATTCA
GGAAAAGTAGAAGACGGAGACGAAATTGTGCAAATAAATTATCAAACTGTTATCGGATGG
CAGTATAAAAAAGTGTTGATGCAATTACA
>g5575.t4 Gene=g5575 Length=269
MAYINVNDWKCEQVIEWLKGIECVNNFYIKAFANNQIGGFQLLYITHTELNELGIKKIGH
QELILEAVEHLKNFHFNLEKENLQYLALQVATTSTSLFKQLSYNPDTKLESVVLKDVAYI
ISKVKTLISWLDNSPFKGHSQYSEMRRNWLKLGLELATIAQRDRFVQNPIKQITSITQTL
SKLSDYIIHDITDPMVLQPAFLDLVTLKKKESELGFYIMPNYQSIHRVTDIKYNSPAHNS
GKVEDGDEIVQINYQTVIGWQYKKVLMQL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g5575.t4 | CDD | cd00992 | PDZ_signaling | 204 | 269 | 0.0000002 |
| 10 | g5575.t4 | Gene3D | G3DSA:1.10.150.50 | Transcription Factor | 2 | 85 | 0.0000000 |
| 9 | g5575.t4 | Gene3D | G3DSA:2.30.42.10 | - | 203 | 269 | 0.0000000 |
| 4 | g5575.t4 | PANTHER | PTHR12844 | CONNECTOR ENCHANCER OF KINASE SUPPRESSOR OF RAS | 1 | 269 | 0.0000000 |
| 5 | g5575.t4 | PANTHER | PTHR12844:SF42 | CONNECTOR ENHANCER OF KSR PROTEIN CNK | 1 | 269 | 0.0000000 |
| 3 | g5575.t4 | Pfam | PF00536 | SAM domain (Sterile alpha motif) | 8 | 72 | 0.0000000 |
| 1 | g5575.t4 | Pfam | PF10534 | Connector enhancer of kinase suppressor of ras | 82 | 163 | 0.0000000 |
| 2 | g5575.t4 | Pfam | PF00595 | PDZ domain | 205 | 265 | 0.0000380 |
| 11 | g5575.t4 | ProSiteProfiles | PS50105 | SAM domain profile. | 9 | 74 | 15.5820000 |
| 13 | g5575.t4 | ProSiteProfiles | PS51290 | CRIC domain profile. | 82 | 167 | 14.2070000 |
| 12 | g5575.t4 | ProSiteProfiles | PS50106 | PDZ domain profile. | 204 | 269 | 12.3450000 |
| 8 | g5575.t4 | SMART | SM00454 | SAM_4 | 6 | 74 | 0.0000000 |
| 6 | g5575.t4 | SUPERFAMILY | SSF47769 | SAM/Pointed domain | 5 | 79 | 0.0000000 |
| 7 | g5575.t4 | SUPERFAMILY | SSF50156 | PDZ domain-like | 176 | 266 | 0.0000000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5575/g5575.t4; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5575.t4.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed