General information for g5647.t21
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as hypothetical.
Parent gene
- The parent gene g5647 is located on chr_2: 10882166..10884280 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5647 | g5647.t21 | TSS | g5647.t21 | 10879963 | 10879963 |
| chr_2 | g5647 | g5647.t21 | isoform | g5647.t21 | 10879968 | 10882810 |
| chr_2 | g5647 | g5647.t21 | exon | g5647.t21.exon1 | 10879968 | 10880133 |
| chr_2 | g5647 | g5647.t21 | exon | g5647.t21.exon2 | 10880615 | 10881023 |
| chr_2 | g5647 | g5647.t21 | cds | g5647.t21.CDS1 | 10880642 | 10881023 |
| chr_2 | g5647 | g5647.t21 | exon | g5647.t21.exon3 | 10881234 | 10881799 |
| chr_2 | g5647 | g5647.t21 | cds | g5647.t21.CDS2 | 10881234 | 10881799 |
| chr_2 | g5647 | g5647.t21 | exon | g5647.t21.exon4 | 10881860 | 10882810 |
| chr_2 | g5647 | g5647.t21 | cds | g5647.t21.CDS3 | 10881860 | 10882810 |
| chr_2 | g5647 | g5647.t21 | TTS | g5647.t21 | 10882977 | 10882977 |
Sequences
>g5647.t21 Gene=g5647 Length=2092
AAATATTTTCAAGTGTCAAGCAATAAACGCGTATAAATTATTGAAAGTGAAAGCAGTAAA
CCAAAGTTGAATTTTTTGAAAAATAATTCGATTGAAAAATTTATGAAAGTACAATAAAGT
ACCAAAGTCAGTAAAGTGATAACATTTTAAAGATTAAATTTAGTTGATAAACCAAAGTTC
TGACTTTCTAAAAATGAATATGAACAAGGGTGATAATAAAGAGAAAATGGGAGGAAAATT
GACTCATGTTGAATTAGAAAGTACTGAAGGTGGAAAAGAAAAGTTGACTCATGTTGAAAT
ACAAGGCACTGGAGGTGGAAAAGGAAGTGATGAAAAGTGTCCAGAAATGAAAGGTGATGT
TCAAAAAACAAGCTCTGAAAGCAAAATTCAAGGTCATTCTGGTATGATGCAAGGAATGAA
AGGTGGCTCACAAGGAAAATGTCCAACAAGAGAATGCAAGGAATTAAAAGGAAAAGTTGA
GAAAGCTAGCTCAGAACATAAGGATAAGAATCAAGCTCATTCCGAATTTATGAAAGAAAT
GGAGAAAAAAGGAAACAATGGAAAATGTCCAGCTCGAAAAGGCACAGAAGGAAAATGTCC
TGCAAGAGAATGTCCAGAATTGAAGGAAAAAGTCGAGAAAGCAAGTTCTGAGCATAAAGA
CAAGCAACGAGCTCATTCCGAATGTATGAAAGAAATGGACAAAAAAGGAACTGGTGGAAA
ATGCCCAGCCCGTGATTGTCCTGAATTAAAAGAAGACTTTCAAAAAGTAAGTTCAGAAAA
GATGAACAAAGAAAAAGCTCATTCAGATTTCAAACAAGAAATGGAAAAAGGAAGAGATGA
CAAATGTAAAGCTCGTGAATGTCCAGAATTAAAATTAAATGTTGAGAAAGCCAACACTCA
AGGAAAAGATCAACATCAAGCTCATACAGAATTTATGCAAGGAATGGGAAAGGGCACAGA
AGGAAAATGCCCTGGTAAAGATTGCCCAGAAATGAAAGGAAAGATTGAAGGCCAGCATCA
TGAACAGCACCATGGACAACATCATGGAGAGCATCATGGAGAACACCATGGACAACATCA
TGGACAACATCATGGAGAGCATCATGGACAACATCATGGAGAGCATCATGGACAACATCA
TGGAGAACACCATGAACAACATCATGGAGAACACCATGGACAACATCATGGAGAGCATCA
TAAGCAAAGTAGTGATCATCATATTGAAATGAAAAGTCCATCAGTAGATTTTGATAATTT
GAAGAAAGTAATTGAAGAAAGATCTAGTGAGGGAAAAGGACATTCTGATTTTAAGCACGA
TGTTGAAAAAGGAGGTTCAAAAAGAATAGGAGAAGAAGATCATCAAGAATTAGGATCTGA
AGGTAAAGGTCATGGCAAGCAACATTCTGATACTAGAAAGCACATGCAGGATCTTAGCTA
TGGTCATGGTAAGGAACATTCGGAATCACAAAAAGGCGAACATATGGGTTTTCATCATAG
AGGACATGGCAAGGAACATTTTGACTCTAAAAGAGGCGATAAAATGGATTCTGAAAGAAA
ACGTCATGAAAAAGAATGCCGTGAAAAAGAATGTCGTCATCATCATAAGAAACAAGGAAA
GGACGTAAAATGGAGAGACTGGAGTGGTGGTGAATTAGCTGAAAGACGTCCACACTTCCC
TTGGAATTGGATGCAAAATTATCATCGTCGTCGTCCAGCAAAAACACCTGACGACTTTGA
AGTTTCTCTCAACGTTGAAGCATTCAAACCTGAGGATATTTCTGTAAAGGTCAGAGGTCG
TGAAATATTGATCGAAGCTAAACATGAAGAAAAGGAAGAAGATGTTGGATTTGTATCTCG
TCATTTTACTCGCAGATATCTTCTTTCTGATGAATTTGATGTTGAATCAGTTGCAACATT
CTTAACACATAATGGAAAAATGACAATTAGAGCAAATAGACCAAAACCAGAAGGTCAAAA
GACTCATCGTGAAGTTCCTATTCAACATGTTGCTTCAGAATCTGATTCTTCTTCTGATGA
AGATGAACACCTTGAGCATATGGTCAAGGAACCGCAATCCTATGGAGTTTAA
>g5647.t21 Gene=g5647 Length=632
MNMNKGDNKEKMGGKLTHVELESTEGGKEKLTHVEIQGTGGGKGSDEKCPEMKGDVQKTS
SESKIQGHSGMMQGMKGGSQGKCPTRECKELKGKVEKASSEHKDKNQAHSEFMKEMEKKG
NNGKCPARKGTEGKCPARECPELKEKVEKASSEHKDKQRAHSECMKEMDKKGTGGKCPAR
DCPELKEDFQKVSSEKMNKEKAHSDFKQEMEKGRDDKCKARECPELKLNVEKANTQGKDQ
HQAHTEFMQGMGKGTEGKCPGKDCPEMKGKIEGQHHEQHHGQHHGEHHGEHHGQHHGQHH
GEHHGQHHGEHHGQHHGEHHEQHHGEHHGQHHGEHHKQSSDHHIEMKSPSVDFDNLKKVI
EERSSEGKGHSDFKHDVEKGGSKRIGEEDHQELGSEGKGHGKQHSDTRKHMQDLSYGHGK
EHSESQKGEHMGFHHRGHGKEHFDSKRGDKMDSERKRHEKECREKECRHHHKKQGKDVKW
RDWSGGELAERRPHFPWNWMQNYHRRRPAKTPDDFEVSLNVEAFKPEDISVKVRGREILI
EAKHEEKEEDVGFVSRHFTRRYLLSDEFDVESVATFLTHNGKMTIRANRPKPEGQKTHRE
VPIQHVASESDSSSDEDEHLEHMVKEPQSYGV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g5647.t21 | CDD | cd06526 | metazoan_ACD | 508 | 589 | 3.7848E-26 |
| 11 | g5647.t21 | Coils | Coil | Coil | 88 | 108 | - |
| 10 | g5647.t21 | Coils | Coil | Coil | 140 | 160 | - |
| 12 | g5647.t21 | Coils | Coil | Coil | 632 | 632 | - |
| 9 | g5647.t21 | Gene3D | G3DSA:2.60.40.790 | - | 496 | 622 | 1.4E-24 |
| 20 | g5647.t21 | MobiDBLite | mobidb-lite | consensus disorder prediction | 22 | 67 | - |
| 15 | g5647.t21 | MobiDBLite | mobidb-lite | consensus disorder prediction | 89 | 180 | - |
| 16 | g5647.t21 | MobiDBLite | mobidb-lite | consensus disorder prediction | 193 | 214 | - |
| 19 | g5647.t21 | MobiDBLite | mobidb-lite | consensus disorder prediction | 273 | 456 | - |
| 21 | g5647.t21 | MobiDBLite | mobidb-lite | consensus disorder prediction | 273 | 296 | - |
| 14 | g5647.t21 | MobiDBLite | mobidb-lite | consensus disorder prediction | 303 | 456 | - |
| 18 | g5647.t21 | MobiDBLite | mobidb-lite | consensus disorder prediction | 586 | 632 | - |
| 17 | g5647.t21 | MobiDBLite | mobidb-lite | consensus disorder prediction | 589 | 625 | - |
| 2 | g5647.t21 | PANTHER | PTHR45640:SF13 | HEAT SHOCK PROTEIN 67B3-RELATED | 468 | 617 | 1.6E-27 |
| 3 | g5647.t21 | PANTHER | PTHR45640 | HEAT SHOCK PROTEIN HSP-12.2-RELATED | 468 | 617 | 1.6E-27 |
| 7 | g5647.t21 | PRINTS | PR00299 | Alpha crystallin signature | 509 | 529 | 2.8E-8 |
| 6 | g5647.t21 | PRINTS | PR00299 | Alpha crystallin signature | 531 | 544 | 2.8E-8 |
| 5 | g5647.t21 | PRINTS | PR00299 | Alpha crystallin signature | 546 | 565 | 2.8E-8 |
| 4 | g5647.t21 | PRINTS | PR00299 | Alpha crystallin signature | 568 | 589 | 2.8E-8 |
| 1 | g5647.t21 | Pfam | PF00011 | Hsp20/alpha crystallin family | 510 | 604 | 4.4E-19 |
| 22 | g5647.t21 | ProSiteProfiles | PS01031 | Small heat shock protein (sHSP) domain profile. | 497 | 604 | 16.093 |
| 8 | g5647.t21 | SUPERFAMILY | SSF49764 | HSP20-like chaperones | 497 | 596 | 1.81E-11 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5647/g5647.t21; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5647.t21.fa.iupred3.txt does not exist
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
- This transcript has been annotated with K19767 (heat shock protein Hsp-16.48/Hsp-16.49)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
