General information for g5732.t21
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as ATP synthase subunit b, mitochondrial.
Parent gene
- The parent gene g5732 is located on chr_2: 11446662..11447330 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5732 | g5732.t21 | TTS | g5732.t21 | 11445921 | 11445921 |
| chr_2 | g5732 | g5732.t21 | isoform | g5732.t21 | 11446010 | 11447330 |
| chr_2 | g5732 | g5732.t21 | exon | g5732.t21.exon1 | 11446010 | 11446177 |
| chr_2 | g5732 | g5732.t21 | cds | g5732.t21.CDS1 | 11446141 | 11446177 |
| chr_2 | g5732 | g5732.t21 | exon | g5732.t21.exon2 | 11446239 | 11446603 |
| chr_2 | g5732 | g5732.t21 | cds | g5732.t21.CDS2 | 11446239 | 11446603 |
| chr_2 | g5732 | g5732.t21 | exon | g5732.t21.exon3 | 11446663 | 11446861 |
| chr_2 | g5732 | g5732.t21 | cds | g5732.t21.CDS3 | 11446663 | 11446861 |
| chr_2 | g5732 | g5732.t21 | exon | g5732.t21.exon4 | 11446929 | 11447052 |
| chr_2 | g5732 | g5732.t21 | cds | g5732.t21.CDS4 | 11446929 | 11447052 |
| chr_2 | g5732 | g5732.t21 | exon | g5732.t21.exon5 | 11447300 | 11447330 |
| chr_2 | g5732 | g5732.t21 | cds | g5732.t21.CDS5 | 11447300 | 11447330 |
| chr_2 | g5732 | g5732.t21 | TSS | g5732.t21 | 11447399 | 11447399 |
Sequences
>g5732.t21 Gene=g5732 Length=887
ATGCTTTCTCGCGCGGCTTTGTTGTCTACAGCCCAAAAGACGTCAGTCATGGCGCTTGCA
ACAAGAGGATCAGCCTCATCAACACCAGGCTTTGAACGACCAGTTCGTCTTGAACATCCT
GGCAAAGTCCGCATGGGATTCATTCCTGATGAATGGTTTAAATTCTTTTATCCAAAAACA
GGTGTCACTGGTCCCTATGTATTCTTAGCTGGTCTCTCAACATACTTGTTTTCAAAGGAA
ATCTATGTTATGGAGCACGAATACTATACTGGACTTTCAATTCTTATTGCTGTCGTTTAT
GCCACGAAGAAATTTGGTCCATCAGTCGCTAAATCACTCGATAAAGAAGTCGATGCTGTT
GAAGAAGAATGGAATCAAGGACGCACTGATGAAATTAAAAGTTTAAATGAAGCCATTGAA
GAAGAAAAGAAGGAACAGTGGAGAGCAGAAGGTCAAATGATGCTTATTGATGCAAAGAAA
GAAAATGTTGCACTTCAGCTTGAAGCTGCCTATCGCGAACGTGCTATGCAAGTCTACCGT
GAAGTCAAGAAACGATTGGATTATCAAGTTGAACGTCAAAATATCGATCGTCGTATTTCA
CAGAAACATATGGCTCAATGGATAGTCAACAATGTCTTGAAATCTATTACACCAGATCAA
GAAGCACAGACACTCAGTAAATGCATTGCTGATTTGGGTGGATTAGCAGCAGCTCGTAAT
GAGAAAGTTCCCGTTATGATAAAAAGTTGTCTCTGAAAAAAAATCTTAACCAACATTTTT
AACGACTTGAATTGTATAAAAAATTGTTTTTCTCCAATTTTCCATCAACAGCATCTATTA
AAAGTGATTGTCGCGACAAGAAATTGCCACCCAAACGCAAATGTTAA
>g5732.t21 Gene=g5732 Length=251
MLSRAALLSTAQKTSVMALATRGSASSTPGFERPVRLEHPGKVRMGFIPDEWFKFFYPKT
GVTGPYVFLAGLSTYLFSKEIYVMEHEYYTGLSILIAVVYATKKFGPSVAKSLDKEVDAV
EEEWNQGRTDEIKSLNEAIEEEKKEQWRAEGQMMLIDAKKENVALQLEAAYRERAMQVYR
EVKKRLDYQVERQNIDRRISQKHMAQWIVNNVLKSITPDQEAQTLSKCIADLGGLAAARN
EKVPVMIKSCL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g5732.t21 | Coils | Coil | Coil | 125 | 152 | - |
| 2 | g5732.t21 | PANTHER | PTHR12733:SF3 | ATP SYNTHASE F(0) COMPLEX SUBUNIT B1, MITOCHONDRIAL | 1 | 239 | 7.8E-92 |
| 3 | g5732.t21 | PANTHER | PTHR12733 | MITOCHONDRIAL ATP SYNTHASE B CHAIN | 1 | 239 | 7.8E-92 |
| 1 | g5732.t21 | Pfam | PF05405 | Mitochondrial ATP synthase B chain precursor (ATP-synt_B) | 73 | 232 | 5.7E-53 |
| 8 | g5732.t21 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 25 | - |
| 9 | g5732.t21 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 10 | g5732.t21 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 16 | - |
| 11 | g5732.t21 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 25 | - |
| 7 | g5732.t21 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 26 | 251 | - |
| 4 | g5732.t21 | SUPERFAMILY | SSF161060 | ATP synthase B chain-like | 111 | 214 | 2.48E-36 |
| 5 | g5732.t21 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 20 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5732/g5732.t21; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5732.t21.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0015986 | ATP synthesis coupled proton transport | BP |
| GO:0000276 | mitochondrial proton-transporting ATP synthase complex, coupling factor F(o) | CC |
| GO:0015078 | proton transmembrane transporter activity | MF |
KEGG
Orthology
- This transcript has been annotated with K02127 (F-type H+-transporting ATPase subunit b)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed