General information for g5856.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Glutathione S-transferase 1, isoform D.
Parent gene
- The parent gene g5856 is located on chr_2: 12432935..12433674 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5856 | g5856.t2 | isoform | g5856.t2 | 12430376 | 12433674 |
| chr_2 | g5856 | g5856.t2 | exon | g5856.t2.exon1 | 12430376 | 12430735 |
| chr_2 | g5856 | g5856.t2 | exon | g5856.t2.exon2 | 12432849 | 12433228 |
| chr_2 | g5856 | g5856.t2 | cds | g5856.t2.CDS1 | 12432935 | 12433228 |
| chr_2 | g5856 | g5856.t2 | exon | g5856.t2.exon3 | 12433291 | 12433458 |
| chr_2 | g5856 | g5856.t2 | cds | g5856.t2.CDS2 | 12433291 | 12433458 |
| chr_2 | g5856 | g5856.t2 | exon | g5856.t2.exon4 | 12433525 | 12433674 |
| chr_2 | g5856 | g5856.t2 | cds | g5856.t2.CDS3 | 12433525 | 12433674 |
| chr_2 | g5856 | g5856.t2 | TTS | g5856.t2 | 12433704 | 12433704 |
| chr_2 | g5856 | g5856.t2 | TSS | g5856.t2 | NA | NA |
Sequences
>g5856.t2 Gene=g5856 Length=1058
AATTGTCAATTAAAAATTTTTTTTCAATTCTTCCATTGAAAAATTTGTCTGTTGTTGCTC
ATATGATGACTTTAGACATACAAATGTCTTTTTCACTCATAAAATAAGTCAAGAAATCTA
AAATTGAGCTAGGCAAGCAAACAGGCAAAAAACTTTCTTCCTGTATTTAACTTTAACCTC
TTTGTAAATAAACTTGTAAATACATCGAATAGACTTAAAAAAAGTAATCTAAAAGTAAAG
GAAATGAATGAGAGAAAGATGAAGAAAAAGAAAGAGGAGGAAAAGGCAACGGCAAACAAT
GTAGAAAGAAAAGTTTAGTTCTGCTTACTTCCTGTCTTGAGTAAATAAAAGATTTCTGTG
TTGTGCTTAGTAATTCATAAAAATAAGTCATGAGTAAAAAATTGTCGGCAATTTTATATC
ATAATTGTGTCAGTCCGCCTTCAAGGATGTGTTTACTTGCAATTCGCAACCTTGGACTAG
ACATTGAAATTCGTAATATCGACATTTACAAAGGAGAACAAAATACACCAGAATTTTTAA
AAATAAATCCATTGCATCAAGTTCCAACTCTCGTTCATGAAGATTTCACTTTAACTGAGT
CAAGAGCAATCATGATGTATCTCGCAACTATCGCAGATTCACCGCTTTATCCACGCAATG
ATTTAAAAAAGCGAGCACTTGTTGATTCAAGATTATTTTATGATGCAACAAATTCATTTG
TCGCTGTCAAAGATTTTGCTCGACCCGTGTTGAGATCGGGAGTGAAAAAAATCTCACCAG
CAGCTAGAGAAGGCATCAAAGTTTTGCTAAGTACACTCGATTCTTTCCTTCAATCATCAG
CATATTTTGCTGGTGAAGAATTAACTTTGGCTGATTTAGCCATTCTTGCTAGTGTTGGAA
CTATCAAATGTTGGGGTGTAAACTTGAAAGAATTTCCAAAACTAAATGATTGGTTTGATC
GTTGCAAGTCATTGCCTGGATTTGCTGAAAATAACGAAGGCTCGCAAATTTTAGCTGAAA
AATTGACAAAATTGTTAGAAGAACCATTATGGAAATAA
>g5856.t2 Gene=g5856 Length=203
MCLLAIRNLGLDIEIRNIDIYKGEQNTPEFLKINPLHQVPTLVHEDFTLTESRAIMMYLA
TIADSPLYPRNDLKKRALVDSRLFYDATNSFVAVKDFARPVLRSGVKKISPAAREGIKVL
LSTLDSFLQSSAYFAGEELTLADLAILASVGTIKCWGVNLKEFPKLNDWFDRCKSLPGFA
ENNEGSQILAEKLTKLLEEPLWK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g5856.t2 | CDD | cd03177 | GST_C_Delta_Epsilon | 74 | 184 | 0.000 |
| 8 | g5856.t2 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 1 | 63 | 0.000 |
| 7 | g5856.t2 | Gene3D | G3DSA:1.20.1050.10 | - | 64 | 200 | 0.000 |
| 3 | g5856.t2 | PANTHER | PTHR43969:SF9 | GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED | 2 | 190 | 0.000 |
| 4 | g5856.t2 | PANTHER | PTHR43969 | GLUTATHIONE S TRANSFERASE D10, ISOFORM A-RELATED | 2 | 190 | 0.000 |
| 2 | g5856.t2 | Pfam | PF02798 | Glutathione S-transferase, N-terminal domain | 2 | 60 | 0.000 |
| 1 | g5856.t2 | Pfam | PF00043 | Glutathione S-transferase, C-terminal domain | 108 | 177 | 0.000 |
| 10 | g5856.t2 | ProSiteProfiles | PS50404 | Soluble glutathione S-transferase N-terminal domain profile. | 1 | 67 | 16.544 |
| 9 | g5856.t2 | ProSiteProfiles | PS50405 | Soluble glutathione S-transferase C-terminal domain profile. | 72 | 196 | 18.110 |
| 12 | g5856.t2 | SFLD | SFLDG00358 | Main (cytGST) | 2 | 175 | 0.000 |
| 13 | g5856.t2 | SFLD | SFLDS00019 | Glutathione Transferase (cytosolic) | 2 | 175 | 0.000 |
| 5 | g5856.t2 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 3 | 69 | 0.000 |
| 6 | g5856.t2 | SUPERFAMILY | SSF47616 | GST C-terminal domain-like | 64 | 187 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5856/g5856.t2; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5856.t2.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006749 | glutathione metabolic process | BP |
| GO:0005515 | protein binding | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed