General information for g5866.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Dual specificity tyrosine-phosphorylation-regulated kinase 2.
Parent gene
- The parent gene g5866 is located on chr_2: 12470266..12484631 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g5866 | g5866.t1 | isoform | g5866.t1 | 12470266 | 12484631 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon1 | 12470266 | 12470507 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS1 | 12470266 | 12470507 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon2 | 12470567 | 12470853 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS2 | 12470567 | 12470853 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon3 | 12471145 | 12471274 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS3 | 12471145 | 12471274 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon4 | 12471354 | 12471497 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS4 | 12471354 | 12471497 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon5 | 12473082 | 12473253 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS5 | 12473082 | 12473253 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon6 | 12473326 | 12473348 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS6 | 12473326 | 12473348 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon7 | 12473427 | 12473542 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS7 | 12473427 | 12473542 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon8 | 12477432 | 12477650 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS8 | 12477432 | 12477650 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon9 | 12478120 | 12478178 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS9 | 12478120 | 12478178 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon10 | 12479746 | 12479909 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS10 | 12479746 | 12479909 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon11 | 12480213 | 12480483 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS11 | 12480213 | 12480483 |
| chr_2 | g5866 | g5866.t1 | exon | g5866.t1.exon12 | 12484614 | 12484631 |
| chr_2 | g5866 | g5866.t1 | cds | g5866.t1.CDS12 | 12484614 | 12484631 |
| chr_2 | g5866 | g5866.t1 | TSS | g5866.t1 | NA | NA |
| chr_2 | g5866 | g5866.t1 | TTS | g5866.t1 | NA | NA |
Sequences
>g5866.t1 Gene=g5866 Length=1845
ATGTTGGATCGTGGACCAGTTCAATTGGAGAACGATAAACTGCGCCGTGAGTCACGTGGA
TCACGAATTGATTTATCAAATTTATGTGTATCATCAAATGCAATGAGACAACATAGCGAT
CAAAAAACGTCAACTTCATCACGTGTCCGTAACACAAATGGTGGCCTCTACATTGACTCA
CAGGGAAACAGTATTCCATTGAGTCCAGTCAGCAGTAGCAATTCATCAAGTAGTGGCAGC
AGTGATAATGTTGTTGAACATCATAAACCTGTTCCAATGACGCCATCAGAAGTTCTAAAG
CAGTATGGCAGTCGATTATGTGACTATGAGAAGCAAGAAATTGAAAAATATCCAGAAATT
TGGTATTTAGGATTAGATGCAAGTAAAGTAAATGCAAAACCTGGCACGCCATTAAATTGC
GGCTATGATGATGAGAATGGAAGTTATAATAAGGTCATTCATGATCATATTTGTTATCGA
TATGAAATACTTGAAGTGATTGGTAAAGGAAGCTTCGGTCAAGTAATCAGAGCATTAGAC
CACAAAACAAATCAGTATGTCGCAATCAAAATAATTCGCAACAAGAAGCGATTTCATCAT
CAAGCACTTGTTGAAGTTCGTATTTTAGATGAACTTCGCAAGAAAGATGCCGATGGATCT
CACAATATTATTCATATGCTGGATTATTTCTACTTCAGGAATCATTTGTGCATTAGTTTT
GAGCTAATGAGCTTGAATCTGTATGAACTTATAAAGAAAAATAATTATCAAGGCTTCAGC
TTGAGTCTTATTCGACGTTTTTGCAATTCAATAGTCAAATGCTTGCGTTTACTCTATCAT
GAGAATGTCATTCATGCTGACCTTAAGCCGGAAAATGTTTTATTGAAGCAAAGAGGAAGC
AGTTCGATAAAAGTGATTGATTTTGGTAGCTCATGCTACTCGCATCGGAAAGTTTATACA
TATATTCAATCACGTTTTTATCGTTCTCCTGAAGTCATTCTTGGACTCTCTTATGGCACT
CCCATCGACATGTGGAGTTTAGGGTGCATTCTTGCAGAACTCTATACCGGATATCCGCTA
TTTCCGGGTGAAAATGAAGTGGAGCAATTAGCTTGTATAATGGAGCTATTGGGCACGCCA
TCGGACGACTTAATAAATCAAGCAACGCGTCGTCGTCTCTTTTTCGATTCGAGAGGCGTT
CCACGTTGCATTACAAATTCAAAAGGTCGCAAACGCAAGCCAGGATCGAAGACATTGTCA
CAATCATTGAGGTGCAATGATACATTATTCATTGATTTTGTCAGCAGATGTCTTGAATGG
GATCCAAAGAAACGTATGACACCAGAAGAAGCAATTCATCATGAATGGCTTCAACCAAGC
AGTTCATCTTCATTTGTTTTATCATCATCAACATCAGCTTCATCACTTCTGAAGCACAAT
CGTAGCGATCAACTAAAAGAAAATGATCAAAGTCAAATTGTGCAAAAATTTCAACGATCT
CAACCCATAACGCCACTAACAATTTTACCACAAATTAAAACACCATCAAATCATCGCAAT
CAAATTAAAACGACTGTGGCAAAGGATGGCAATCGAATTAAAGTTCTTGGAACATCAACA
GAATTAGAAGTGTCATCACAAAAACAGTGCAGTTATTATCAACAGCAACAACAACCACAT
TTAAGAAAGCTTGCAACAACAATTAATAACAATGCAATGCAACATTCAACAATTTCAAAC
ACTTCCTCATCATCGTCATCATCGATCAATAGCTCCAGCAAGTATGGTATGTCACATTCG
CAAAGCACGGGCGATGTTACAGCAGCCATATTTGGAAAAGCGTGA
>g5866.t1 Gene=g5866 Length=614
MLDRGPVQLENDKLRRESRGSRIDLSNLCVSSNAMRQHSDQKTSTSSRVRNTNGGLYIDS
QGNSIPLSPVSSSNSSSSGSSDNVVEHHKPVPMTPSEVLKQYGSRLCDYEKQEIEKYPEI
WYLGLDASKVNAKPGTPLNCGYDDENGSYNKVIHDHICYRYEILEVIGKGSFGQVIRALD
HKTNQYVAIKIIRNKKRFHHQALVEVRILDELRKKDADGSHNIIHMLDYFYFRNHLCISF
ELMSLNLYELIKKNNYQGFSLSLIRRFCNSIVKCLRLLYHENVIHADLKPENVLLKQRGS
SSIKVIDFGSSCYSHRKVYTYIQSRFYRSPEVILGLSYGTPIDMWSLGCILAELYTGYPL
FPGENEVEQLACIMELLGTPSDDLINQATRRRLFFDSRGVPRCITNSKGRKRKPGSKTLS
QSLRCNDTLFIDFVSRCLEWDPKKRMTPEEAIHHEWLQPSSSSSFVLSSSTSASSLLKHN
RSDQLKENDQSQIVQKFQRSQPITPLTILPQIKTPSNHRNQIKTTVAKDGNRIKVLGTST
ELEVSSQKQCSYYQQQQQPHLRKLATTINNNAMQHSTISNTSSSSSSSINSSSKYGMSHS
QSTGDVTAAIFGKA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g5866.t1 | CDD | cd14225 | PKc_DYRK4 | 117 | 457 | 0.0 |
| 6 | g5866.t1 | Gene3D | G3DSA:1.10.8.980 | - | 31 | 135 | 5.0E-15 |
| 5 | g5866.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 143 | 242 | 4.7E-122 |
| 7 | g5866.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 217 | 457 | 4.7E-122 |
| 12 | g5866.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 28 | 84 | - |
| 14 | g5866.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 28 | 92 | - |
| 13 | g5866.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 576 | 603 | - |
| 2 | g5866.t1 | PANTHER | PTHR24058 | DUAL SPECIFICITY PROTEIN KINASE | 33 | 481 | 5.7E-196 |
| 3 | g5866.t1 | PANTHER | PTHR24058:SF22 | DUAL SPECIFICITY TYROSINE-PHOSPHORYLATION-REGULATED KINASE 4 | 33 | 481 | 5.7E-196 |
| 1 | g5866.t1 | Pfam | PF00069 | Protein kinase domain | 161 | 457 | 5.9E-58 |
| 10 | g5866.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 167 | 190 | - |
| 9 | g5866.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 283 | 295 | - |
| 15 | g5866.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 161 | 457 | 40.428 |
| 11 | g5866.t1 | SMART | SM00220 | serkin_6 | 161 | 457 | 4.0E-86 |
| 4 | g5866.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 152 | 465 | 3.51E-86 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
Data is missing for g5866/g5866.t1; file /home/yuki.yoshida/nias/analysis/reanalysis/18_revice/midgebase/iupred3/g5866.t1.fa.iupred3.txt does not exist
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K18669 (dual specificity tyrosine-phosphorylation-regulated kinase 2/3/4)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
