General information for g602.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Prosaposin.
Parent gene
- The parent gene g602 is located on chr_3: 4437608..4441569 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g602 | g602.t1 | TSS | g602.t1 | 4434335 | 4434335 |
| chr_3 | g602 | g602.t1 | isoform | g602.t1 | 4434385 | 4437608 |
| chr_3 | g602 | g602.t1 | exon | g602.t1.exon1 | 4434385 | 4434424 |
| chr_3 | g602 | g602.t1 | cds | g602.t1.CDS1 | 4434385 | 4434424 |
| chr_3 | g602 | g602.t1 | exon | g602.t1.exon2 | 4434520 | 4434598 |
| chr_3 | g602 | g602.t1 | cds | g602.t1.CDS2 | 4434520 | 4434598 |
| chr_3 | g602 | g602.t1 | exon | g602.t1.exon3 | 4434886 | 4435168 |
| chr_3 | g602 | g602.t1 | cds | g602.t1.CDS3 | 4434886 | 4435168 |
| chr_3 | g602 | g602.t1 | exon | g602.t1.exon4 | 4435272 | 4437497 |
| chr_3 | g602 | g602.t1 | cds | g602.t1.CDS4 | 4435272 | 4437497 |
| chr_3 | g602 | g602.t1 | exon | g602.t1.exon5 | 4437561 | 4437608 |
| chr_3 | g602 | g602.t1 | cds | g602.t1.CDS5 | 4437561 | 4437608 |
| chr_3 | g602 | g602.t1 | TTS | g602.t1 | 4437776 | 4437776 |
Sequences
>g602.t1 Gene=g602 Length=2676
ATGAAGATTTTGTTCTCAATTATTTGTACTTTTTTGTTCGCGTCTATTGTATCTAGTTCC
GTTATATTGGGACGAGAAAAATGCACATACGGGCCCTCATATTGGTGTAATAACTTAGTA
AATGCAAAAAATTGCGGAGCAGTTAAACACTGTATTAAAACAGTTTGGGAAAAACAACAT
GTACCGGAAGACACTGATTCTATTTGCAAAATTTGTCTTGACATGGTAACCCAAGCCAGA
GATCAACTCGAAAGCAATGAAACTCAAGAAGAGTTGAAAGAAGTTTTCGATGGTTCTTGC
GATTTGATACCGATAAAAGTAGTAAAAACGGAATGCAAACGTTTGGCTGATGATTTCATA
CCAGAACTTGTTGAGGCACTTGCTTCTCAAATGAATCCACAGGTCGTTTGTTCTACAGCT
GGACTTTGTAATAATGCAAAGATTGATGCATTATTGGAAGAATATGAAATGCAGAAGAAA
AATAATTTATTAAGTTGCAACCAATGTAACAACATTGGAGAATTGATCACTCAAAAATTC
CATACATCATCTCGTGATCAAGTTCTAGATGGTTTCTTGAGTGCCTGCGGTCGTCTTTCA
TCGTTTTCTGATGCATGTTCTAGTATCATTCTGACTTATTTCAACGAAATCTATGAAGAA
CTTTCGAGAAATCTAAATTCTGATAGTATTTGTCATATATCTGGCGTATGCGCAAGCAAA
TATCATAATCATGCATCAGCAGAAGTTGAAATTGAAGTAAAATCAAATGTTGGAGTACTT
AAGAATAAAGAGCTCAAAGATGATATTCCTTGTGAATTATGTGAACAGTTGGTCAAACAT
TTAAGTGATATTCTTGTTGCAAATACAACTGAAACTGAATTTAAAAGCGTCCTTCAAGGT
CTTTGTTCACAAACAAAAGGATTCAAGAGCGAATGTTTAGAACTGGTAAATCAATATTAT
GCAATTATTTACGAGTCTCTCGTTAATAATCTCGACGCAAATGGAGCTTGCTTTTTAATT
GGAGTATGTCGCAAGGGAGATAATAATCATCAAGTCACTCCACACATGCCATTATTGCCT
ACTAATCAAAAGAATATACCAAAGCGCAATCGATTAGGAGAGAATGAAAAATCCTATACA
AATGAGCAAATCAAGAAAATGATTTTGCCTATTGATCAATTAATGGGAGCTAAATCAAAC
CTTGATTTGGTTGATGGTGGTGAAAAGTGTACTCTTTGTCAATATTTTCTTCATTTTGTT
CAAGAAGAATTAAGTGATGCAAAAAATGAAGACGAAATTAAAGAGATAGTAGGAAAAACT
TGTGACAAATTCTCATCCGTAATTCGTTCAAATTGTCATCAATTTGTTAATTTATATGGA
GATGCAATAATTGCTTTATTAGTTCAAAGCATTGATCCTCGCGATGTATGTCCCAATTTA
AAATTATGTCCGAAATTCATGAATCAAGACGTTGATGTTTTTGCACCTTATCCGATCGTT
GAAGTAAATGAATCCAGCAAAACGCAATGTCCATTGTGTGTTCTTGTTGTAAAAGAGGCT
CAAGATTATATAAAAAGTGAGAAAACTACTGAAACCGTAAAGAAAGCACTAGAAAGAGCG
TGTTCGAGACTCCCAGCAAAATTACAATTGCAATGTAAAGATTTTGTGGAAACTTATTAC
GATGAATTACTTCAAAAGTTGCTCTCTGATTTCACTCCAAAAGACATTTGCATTGATTTG
CAATTGTGCACAAGAATTCAAGATGAAATAAAAGTTGGAATTGAAAAAGTCGAACATATT
CCAAACATCAAGACAAATGAAATTCCTGATTATACTATCAACGGTCAATATTTTACAAGT
TCTGATTCTGGAGAGTGCTTCTTGTGTACAACCGTTTTAAGTAACGCTGAGAGCAAAATT
ACTCATGGAATGACTAAGAATCAAATTGAAGATATTTTATTACGCGAATGCTCAGTTTAT
TATATTTATGAAGGCGTCTGCGATAATTTTGTTAAAACGAATATTGATCAAATATTCTCT
TTAGTAGAACAAAAACTTTCTCCTAAGCAAGTTTGTCAGAATTTATCTCTCTGTGCATCA
ACAGACGAGTTAAATATTGATGAAGCAGTTATTGTAAATGTTGTGGCATTACCTGCATTC
CCAACTGAAAAGAAAAATGAATTTAATCGTGTTAAAATTATAAGAGGAGATGATGATGAT
GATGATGATGATGACGACGACGATGATGATGATGATGATGATGATGATGATGATCCAAAG
AAAAAACCTTCATTCAAAGATGATCCAATTTGTGTACTTTGCGAATTTGTAATGACAAAA
TTAGAATCTGAATTGAAAAATAAAAAAACACAGGAAGAAATCAAAGAGGCTGTTGAGAAT
ATTTGCTCAAAAATGCCGAAATCTGTTTCTAAGCAATGTACAAAATTCGTTGATCAATAC
AGTGAACTCATCATTGCATTGATTGATACTGTACCACCCAAAGAAATTTGTTCACAAATG
GGCTTATGTACAGCTGTAAAAAAACAAATGCACCTCGTGGGTGCAAATGAGTGCACTTAT
GGTCCAACTCACTTTTGTTCTGATATGAAAATTGCTGAAAAATGCAAGGCTACCAAATAC
TGTAAGGAAAAGAAGTTGGGAAAATTTTCAGTTTGA
>g602.t1 Gene=g602 Length=891
MKILFSIICTFLFASIVSSSVILGREKCTYGPSYWCNNLVNAKNCGAVKHCIKTVWEKQH
VPEDTDSICKICLDMVTQARDQLESNETQEELKEVFDGSCDLIPIKVVKTECKRLADDFI
PELVEALASQMNPQVVCSTAGLCNNAKIDALLEEYEMQKKNNLLSCNQCNNIGELITQKF
HTSSRDQVLDGFLSACGRLSSFSDACSSIILTYFNEIYEELSRNLNSDSICHISGVCASK
YHNHASAEVEIEVKSNVGVLKNKELKDDIPCELCEQLVKHLSDILVANTTETEFKSVLQG
LCSQTKGFKSECLELVNQYYAIIYESLVNNLDANGACFLIGVCRKGDNNHQVTPHMPLLP
TNQKNIPKRNRLGENEKSYTNEQIKKMILPIDQLMGAKSNLDLVDGGEKCTLCQYFLHFV
QEELSDAKNEDEIKEIVGKTCDKFSSVIRSNCHQFVNLYGDAIIALLVQSIDPRDVCPNL
KLCPKFMNQDVDVFAPYPIVEVNESSKTQCPLCVLVVKEAQDYIKSEKTTETVKKALERA
CSRLPAKLQLQCKDFVETYYDELLQKLLSDFTPKDICIDLQLCTRIQDEIKVGIEKVEHI
PNIKTNEIPDYTINGQYFTSSDSGECFLCTTVLSNAESKITHGMTKNQIEDILLRECSVY
YIYEGVCDNFVKTNIDQIFSLVEQKLSPKQVCQNLSLCASTDELNIDEAVIVNVVALPAF
PTEKKNEFNRVKIIRGDDDDDDDDDDDDDDDDDDDDDDPKKKPSFKDDPICVLCEFVMTK
LESELKNKKTQEEIKEAVENICSKMPKSVSKQCTKFVDQYSELIIALIDTVPPKEICSQM
GLCTAVKKQMHLVGANECTYGPTHFCSDMKIAEKCKATKYCKEKKLGKFSV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 42 | g602.t1 | Coils | Coil | Coil | 780 | 800 | - |
| 37 | g602.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 63 | 146 | 3.7E-19 |
| 41 | g602.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 162 | 246 | 1.1E-10 |
| 40 | g602.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 265 | 345 | 5.2E-17 |
| 39 | g602.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 406 | 483 | 4.0E-21 |
| 35 | g602.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 505 | 585 | 1.4E-20 |
| 38 | g602.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 621 | 704 | 9.3E-16 |
| 36 | g602.t1 | Gene3D | G3DSA:1.10.225.10 | Saposin | 765 | 845 | 3.7E-23 |
| 57 | g602.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 737 | 762 | - |
| 58 | g602.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 738 | 757 | - |
| 14 | g602.t1 | PANTHER | PTHR11480 | SAPOSIN-RELATED | 9 | 353 | 3.9E-164 |
| 17 | g602.t1 | PANTHER | PTHR11480:SF84 | BCDNA.GH08312 | 9 | 353 | 3.9E-164 |
| 16 | g602.t1 | PANTHER | PTHR11480 | SAPOSIN-RELATED | 386 | 589 | 3.9E-164 |
| 19 | g602.t1 | PANTHER | PTHR11480:SF84 | BCDNA.GH08312 | 386 | 589 | 3.9E-164 |
| 15 | g602.t1 | PANTHER | PTHR11480 | SAPOSIN-RELATED | 621 | 846 | 3.9E-164 |
| 18 | g602.t1 | PANTHER | PTHR11480:SF84 | BCDNA.GH08312 | 621 | 846 | 3.9E-164 |
| 24 | g602.t1 | PRINTS | PR01797 | Saposin signature | 21 | 45 | 1.8E-19 |
| 20 | g602.t1 | PRINTS | PR01797 | Saposin signature | 50 | 70 | 1.8E-19 |
| 23 | g602.t1 | PRINTS | PR01797 | Saposin signature | 133 | 151 | 1.8E-19 |
| 25 | g602.t1 | PRINTS | PR01797 | Saposin signature | 407 | 429 | 1.8E-19 |
| 21 | g602.t1 | PRINTS | PR01797 | Saposin signature | 439 | 461 | 1.8E-19 |
| 22 | g602.t1 | PRINTS | PR01797 | Saposin signature | 461 | 484 | 1.8E-19 |
| 13 | g602.t1 | Pfam | PF02199 | Saposin A-type domain | 26 | 57 | 1.8E-15 |
| 4 | g602.t1 | Pfam | PF05184 | Saposin-like type B, region 1 | 68 | 104 | 7.2E-9 |
| 11 | g602.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 110 | 143 | 5.2E-9 |
| 1 | g602.t1 | Pfam | PF05184 | Saposin-like type B, region 1 | 270 | 304 | 1.2E-6 |
| 7 | g602.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 311 | 343 | 2.6E-5 |
| 3 | g602.t1 | Pfam | PF05184 | Saposin-like type B, region 1 | 409 | 444 | 3.0E-7 |
| 10 | g602.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 452 | 483 | 1.4E-7 |
| 5 | g602.t1 | Pfam | PF05184 | Saposin-like type B, region 1 | 509 | 545 | 1.3E-10 |
| 8 | g602.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 551 | 583 | 1.3E-9 |
| 9 | g602.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 666 | 698 | 3.3E-8 |
| 2 | g602.t1 | Pfam | PF05184 | Saposin-like type B, region 1 | 769 | 806 | 2.0E-13 |
| 6 | g602.t1 | Pfam | PF03489 | Saposin-like type B, region 2 | 811 | 843 | 1.5E-9 |
| 12 | g602.t1 | Pfam | PF02199 | Saposin A-type domain | 856 | 884 | 1.1E-8 |
| 44 | g602.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 19 | - |
| 45 | g602.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 46 | g602.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 14 | - |
| 47 | g602.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 19 | - |
| 43 | g602.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 20 | 891 | - |
| 60 | g602.t1 | ProSiteProfiles | PS51110 | Saposin A-type domain profile. | 21 | 61 | 14.931 |
| 67 | g602.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 65 | 147 | 20.297 |
| 65 | g602.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 162 | 241 | 11.733 |
| 64 | g602.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 267 | 347 | 15.929 |
| 61 | g602.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 406 | 487 | 17.318 |
| 66 | g602.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 506 | 587 | 19.58 |
| 63 | g602.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 622 | 702 | 15.258 |
| 62 | g602.t1 | ProSiteProfiles | PS50015 | Saposin B type domain profile. | 767 | 847 | 19.907 |
| 59 | g602.t1 | ProSiteProfiles | PS51110 | Saposin A-type domain profile. | 851 | 891 | 10.22 |
| 48 | g602.t1 | SMART | SM00162 | sapA_2 | 24 | 57 | 1.6E-13 |
| 52 | g602.t1 | SMART | SM00741 | sapb_4 | 67 | 143 | 2.2E-22 |
| 55 | g602.t1 | SMART | SM00741 | sapb_4 | 164 | 237 | 1.2E-5 |
| 54 | g602.t1 | SMART | SM00741 | sapb_4 | 269 | 343 | 1.0E-15 |
| 50 | g602.t1 | SMART | SM00741 | sapb_4 | 408 | 483 | 4.0E-19 |
| 51 | g602.t1 | SMART | SM00741 | sapb_4 | 508 | 583 | 2.1E-22 |
| 56 | g602.t1 | SMART | SM00741 | sapb_4 | 624 | 698 | 1.2E-15 |
| 53 | g602.t1 | SMART | SM00741 | sapb_4 | 769 | 843 | 1.3E-25 |
| 49 | g602.t1 | SMART | SM00162 | sapA_2 | 854 | 887 | 0.0074 |
| 30 | g602.t1 | SUPERFAMILY | SSF47862 | Saposin | 66 | 145 | 2.24E-17 |
| 28 | g602.t1 | SUPERFAMILY | SSF47862 | Saposin | 202 | 303 | 3.45E-14 |
| 27 | g602.t1 | SUPERFAMILY | SSF47862 | Saposin | 308 | 444 | 1.57E-16 |
| 26 | g602.t1 | SUPERFAMILY | SSF47862 | Saposin | 449 | 545 | 3.93E-19 |
| 32 | g602.t1 | SUPERFAMILY | SSF47862 | Saposin | 550 | 658 | 1.73E-12 |
| 29 | g602.t1 | SUPERFAMILY | SSF47862 | Saposin | 663 | 806 | 4.21E-20 |
| 31 | g602.t1 | SUPERFAMILY | SSF47862 | Saposin | 809 | 876 | 1.37E-9 |
| 34 | g602.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 19 | - |
| 33 | g602.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 24 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006665 | sphingolipid metabolic process | BP |
| GO:0005764 | lysosome | CC |
| GO:0006629 | lipid metabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K12382 (saposin)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
