General information for g6050.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative fatty acyl-CoA reductase CG5065.
Parent gene
- The parent gene g6050 is located on chr_2: 13867542..13868057 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6050 | g6050.t1 | TSS | g6050.t1 | 13864562 | 13864562 |
| chr_2 | g6050 | g6050.t1 | isoform | g6050.t1 | 13864662 | 13867778 |
| chr_2 | g6050 | g6050.t1 | exon | g6050.t1.exon1 | 13864662 | 13864859 |
| chr_2 | g6050 | g6050.t1 | cds | g6050.t1.CDS1 | 13864662 | 13864859 |
| chr_2 | g6050 | g6050.t1 | exon | g6050.t1.exon2 | 13866362 | 13866785 |
| chr_2 | g6050 | g6050.t1 | cds | g6050.t1.CDS2 | 13866362 | 13866785 |
| chr_2 | g6050 | g6050.t1 | exon | g6050.t1.exon3 | 13866882 | 13867167 |
| chr_2 | g6050 | g6050.t1 | cds | g6050.t1.CDS3 | 13866882 | 13867167 |
| chr_2 | g6050 | g6050.t1 | exon | g6050.t1.exon4 | 13867226 | 13867465 |
| chr_2 | g6050 | g6050.t1 | cds | g6050.t1.CDS4 | 13867226 | 13867465 |
| chr_2 | g6050 | g6050.t1 | exon | g6050.t1.exon5 | 13867525 | 13867656 |
| chr_2 | g6050 | g6050.t1 | cds | g6050.t1.CDS5 | 13867525 | 13867656 |
| chr_2 | g6050 | g6050.t1 | exon | g6050.t1.exon6 | 13867712 | 13867778 |
| chr_2 | g6050 | g6050.t1 | cds | g6050.t1.CDS6 | 13867712 | 13867778 |
| chr_2 | g6050 | g6050.t1 | TTS | g6050.t1 | 13868160 | 13868160 |
Sequences
>g6050.t1 Gene=g6050 Length=1347
ATGAAGGAATTAAATTCAGTGCAAGAATTTTATAAAGACAAAATAATCTTCATCACGGGT
GGCTCAGGATTTATGGGAAAAGTTCTGATTGAAAAGCTGCTCTATTCATGTTCTGATTTA
AAGCAATTAATTTTATTAATGAGAGTAAAGAAAGGAAAAAGTGAAATGGCAAGAGTTAAA
GAATTTGAAGCACTTCCTATGTTCAAACGAATAATGGAAGAAAAGCCGGAAGTGATGAAG
AAAATATTAGTAGTTTCTGGTGAAATTACTGAAAAGAATTTGGGATTAAGTGAAGAAAAT
CTTCAATATGTGATTGATAATACGGATATAGTTTTCCATGTAGCAGCATCAATTCGATTT
ACAGCTCCACTTAAAGAAAATGTTCTTACAAATTTGGTTGCAACACAGGAAACTATTGAT
ATTGCAAAAAAGATGAAAAATTTGAAAGCAATGTTGCATGTTTCAACTGCATTTTGCACA
GTAGAAAGATCAGTAATTGATGAGAAAGTTTACGACCATCCAGGAAATCCATTTAATTTA
ATTTCATTAGCAAAACAAATGGATGAACCAACAATGTCAGGACTTGAAGATCAATTGGTT
CCACCTCATCCAAATACTTATACTTACACAAAAAGATTAGCAGAACTTCTCGTTCAAGAT
GAATACAAAAATTCTAATCTACCAATTTGTATTGTAAGACCCAGCATTGTAGGACCAGCG
CTAAAAGAACCTATGCCTGGATGGGTTGATTCACTTGGTGCAATTAGTGGAATAATTTTT
GGTGTTGCTAAAGGAGTCATTCGATGTATGCTAATTGATCCTGAGAGTAATTTTGAGTAC
ATTCCAGTCGATCAATCAATCAATGGATTTATTATGGTAGCAAAACAAATTGGAACACAA
AACAAAAGATCTGAAGAAATTCCCGTTTATAATATGACAGCAGAAGAAAATGGATTGTTT
ACATATCAAAAATTTTATGATTGGATGAAATCTTTAATTTTTAAGTTACCATTTTTAGAA
TCACTTTGGTATGTCAATATTACAATGACGATGAATGAAAAATATTTTTGGTTCAATGTC
TACATGTTCCAAATGTTACCAGCTTTATTGATAGACTTTGCATTAATGATCTTAGGAAAA
AAGAGATATTTAGTTGATGTTCAACGAAAGGTGATCAAATCATCTTATTTACTCAGAGCA
TTTACAGCAAAGAAATGGAAATTTTATACGAAGAACTTTCATAATCTTATGTTGATTCAA
TCACCCGAAGAAAAGGAAATGTTCAACATTGACACAAATGCAAATGATCCTGTTCAATAT
TTCAAAGACAGTGTCATTGGAGTTTGA
>g6050.t1 Gene=g6050 Length=448
MKELNSVQEFYKDKIIFITGGSGFMGKVLIEKLLYSCSDLKQLILLMRVKKGKSEMARVK
EFEALPMFKRIMEEKPEVMKKILVVSGEITEKNLGLSEENLQYVIDNTDIVFHVAASIRF
TAPLKENVLTNLVATQETIDIAKKMKNLKAMLHVSTAFCTVERSVIDEKVYDHPGNPFNL
ISLAKQMDEPTMSGLEDQLVPPHPNTYTYTKRLAELLVQDEYKNSNLPICIVRPSIVGPA
LKEPMPGWVDSLGAISGIIFGVAKGVIRCMLIDPESNFEYIPVDQSINGFIMVAKQIGTQ
NKRSEEIPVYNMTAEENGLFTYQKFYDWMKSLIFKLPFLESLWYVNITMTMNEKYFWFNV
YMFQMLPALLIDFALMILGKKRYLVDVQRKVIKSSYLLRAFTAKKWKFYTKNFHNLMLIQ
SPEEKEMFNIDTNANDPVQYFKDSVIGV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g6050.t1 | CDD | cd05236 | FAR-N_SDR_e | 14 | 337 | 1.69701E-107 |
| 12 | g6050.t1 | CDD | cd09071 | FAR_C | 362 | 448 | 2.96503E-22 |
| 7 | g6050.t1 | Gene3D | G3DSA:3.40.50.720 | - | 3 | 352 | 1.9E-62 |
| 3 | g6050.t1 | PANTHER | PTHR11011 | MALE STERILITY PROTEIN 2-RELATED | 3 | 447 | 5.8E-153 |
| 4 | g6050.t1 | PANTHER | PTHR11011:SF12 | FATTY ACYL-COA REDUCTASE | 3 | 447 | 5.8E-153 |
| 1 | g6050.t1 | Pfam | PF07993 | Male sterility protein | 18 | 288 | 9.3E-70 |
| 2 | g6050.t1 | Pfam | PF03015 | Male sterility protein | 363 | 448 | 1.6E-18 |
| 9 | g6050.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 354 | - |
| 10 | g6050.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 355 | 379 | - |
| 8 | g6050.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 380 | 448 | - |
| 6 | g6050.t1 | SUPERFAMILY | SSF51735 | NAD(P)-binding Rossmann-fold domains | 6 | 299 | 1.84E-27 |
| 5 | g6050.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 357 | 379 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0080019 | fatty-acyl-CoA reductase (alcohol-forming) activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed