General information for g6053.t20
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Alpha-amylase.
Parent gene
- The parent gene g6053 is located on chr_2: 13891629..13893158 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6053 | g6053.t20 | TSS | g6053.t20 | 13890242 | 13890242 |
| chr_2 | g6053 | g6053.t20 | isoform | g6053.t20 | 13890291 | 13892251 |
| chr_2 | g6053 | g6053.t20 | exon | g6053.t20.exon1 | 13890291 | 13890343 |
| chr_2 | g6053 | g6053.t20 | cds | g6053.t20.CDS1 | 13890291 | 13890343 |
| chr_2 | g6053 | g6053.t20 | exon | g6053.t20.exon2 | 13890409 | 13890509 |
| chr_2 | g6053 | g6053.t20 | cds | g6053.t20.CDS2 | 13890409 | 13890509 |
| chr_2 | g6053 | g6053.t20 | exon | g6053.t20.exon3 | 13890563 | 13890632 |
| chr_2 | g6053 | g6053.t20 | cds | g6053.t20.CDS3 | 13890563 | 13890632 |
| chr_2 | g6053 | g6053.t20 | exon | g6053.t20.exon4 | 13890692 | 13890915 |
| chr_2 | g6053 | g6053.t20 | cds | g6053.t20.CDS4 | 13890692 | 13890915 |
| chr_2 | g6053 | g6053.t20 | exon | g6053.t20.exon5 | 13890977 | 13891198 |
| chr_2 | g6053 | g6053.t20 | cds | g6053.t20.CDS5 | 13890977 | 13891194 |
| chr_2 | g6053 | g6053.t20 | exon | g6053.t20.exon6 | 13891362 | 13891506 |
| chr_2 | g6053 | g6053.t20 | exon | g6053.t20.exon7 | 13891629 | 13891972 |
| chr_2 | g6053 | g6053.t20 | exon | g6053.t20.exon8 | 13892097 | 13892251 |
| chr_2 | g6053 | g6053.t20 | TTS | g6053.t20 | 13892345 | 13892345 |
Sequences
>g6053.t20 Gene=g6053 Length=1314
ATGAAACTTTTAGTTGTAATTTTGAGCATTTTTGTAGCTGTCAATGCTCAATGGGACACT
CATTGGTGGAGTGGTCGAAGTGGAATTGTTCATTTATTTGAGTGGAAATGGAATGATATT
GCAAATGAATGTGAAACATTCTTAGCACCAAATGGTTACGCGGGAGTCCAAACTTCACCT
CCGTCAGAAAATTTAGTCATCGGAAATCGACCCTGGTGGGAAAGATATCAGCCAGTTTCA
TATCATTTGAATACACGATCAGGTGATAGAAATGCATTCGCTGATATGGTTAGACGATGC
AATGCTGTTGGTATTCGTATCTATCCTGATGTTGTAATTAATCATATGGCGGCTGGTGAT
GGTTATGGAACAGGCGGAAGTGAATCTAGAGTTAGCCAATTAAGCTTCCCAGCTGTTCCA
TATGGAAGTAATGACTTCAATCCAAGATGTGATATCACTAATTACAATGATCCATATCAA
GTTAGAAACTGCTGGCTTGTTGGTTTATCTGATCTTAAGACAGGAAGTGATTATGTCCGT
CAGAAAATTGCCGATTTCTTAAATGATTTGATTAGTCTTGGTGTTGCAGGTAATTTCGTC
TTAATTTTAAGTGTTTTCGACGAAATAAAAAATATTTCTTATCTCTTATCAAGGTTTTCG
TATTGATGCCGAAGTTATTGATTTGGGTGGTGAAGCAATTAAAAAAGACGAATATTTGCA
CATTGGAACAATCACAGAGTTTAGATACAGTGCTGAAATTGGACGAGTTTTCCGTGGTTA
TGATTTATTGAAATATCTTAAGAACTTTGGTGAAGGATGGGGTTTTATGGCATCAGCATC
AGCTCTTACTTTTGTTGACAATCACGACAACCAGAGAGGACATGGAGCGGGAGGTGCCAA
TGTTCTCACATATAAAGTCTCAAAGAATTACAAAATGGCAACAGCTTTCCATCTCGCATG
GAATTACGGAATTCCTCGTATCATGAGTAGCTTTGCTTTCAATGATGGCGATCAAGGTCC
ACCAGCTGATGGCTATGGTAATTTGAAATCACCAGAATTTAATTCAGAAGGAGCTTGCAC
TAATGGATGGGTTTGCGAACATCGTTGGAGACAAATTTACAACATGGTTAAATTCCGTAA
TGCAGCTGGTGGTGCAGCTCTGGAACTTATTGTGAAATTGCATCAGGAAGCAAGAGCGGT
TCAAGTTGCACAGGAAAAACAATTACAGTCGATGGCAGTGGAAATGCCTACATTGATTTA
CCACACAATGCTGACGACGGTTTTGTTGCAATTCATGTTGATGCGAAATTGTAA
>g6053.t20 Gene=g6053 Length=221
MKLLVVILSIFVAVNAQWDTHWWSGRSGIVHLFEWKWNDIANECETFLAPNGYAGVQTSP
PSENLVIGNRPWWERYQPVSYHLNTRSGDRNAFADMVRRCNAVGIRIYPDVVINHMAAGD
GYGTGGSESRVSQLSFPAVPYGSNDFNPRCDITNYNDPYQVRNCWLVGLSDLKTGSDYVR
QKIADFLNDLISLGVAGNFVLILSVFDEIKNISYLLSRFSY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g6053.t20 | Gene3D | G3DSA:3.20.20.80 | Glycosidases | 18 | 200 | 1.6E-84 |
| 2 | g6053.t20 | PANTHER | PTHR43447 | ALPHA-AMYLASE | 16 | 197 | 1.2E-66 |
| 3 | g6053.t20 | PANTHER | PTHR43447:SF7 | ALPHA-AMYLASE | 16 | 197 | 1.2E-66 |
| 5 | g6053.t20 | PRINTS | PR00110 | Alpha-amylase signature | 72 | 89 | 1.7E-9 |
| 4 | g6053.t20 | PRINTS | PR00110 | Alpha-amylase signature | 104 | 115 | 1.7E-9 |
| 1 | g6053.t20 | Pfam | PF00128 | Alpha amylase, catalytic domain | 82 | 197 | 6.9E-5 |
| 10 | g6053.t20 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 11 | g6053.t20 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 12 | g6053.t20 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 11 | - |
| 13 | g6053.t20 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 9 | g6053.t20 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 221 | - |
| 15 | g6053.t20 | SMART | SM00642 | aamy | 27 | 218 | 7.6E-8 |
| 6 | g6053.t20 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 19 | 197 | 6.63E-51 |
| 7 | g6053.t20 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
| 14 | g6053.t20 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0043169 | cation binding | MF |
| GO:0004556 | alpha-amylase activity | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed