General information for g6077.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 4V2.
Parent gene
- The parent gene g6077 is located on chr_2: 14022599..14024890 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6077 | g6077.t1 | isoform | g6077.t1 | 14022599 | 14024890 |
| chr_2 | g6077 | g6077.t1 | exon | g6077.t1.exon1 | 14022599 | 14022777 |
| chr_2 | g6077 | g6077.t1 | cds | g6077.t1.CDS1 | 14022599 | 14022777 |
| chr_2 | g6077 | g6077.t1 | exon | g6077.t1.exon2 | 14022835 | 14023249 |
| chr_2 | g6077 | g6077.t1 | cds | g6077.t1.CDS2 | 14022835 | 14023249 |
| chr_2 | g6077 | g6077.t1 | exon | g6077.t1.exon3 | 14023972 | 14024236 |
| chr_2 | g6077 | g6077.t1 | cds | g6077.t1.CDS3 | 14023972 | 14024236 |
| chr_2 | g6077 | g6077.t1 | exon | g6077.t1.exon4 | 14024292 | 14024890 |
| chr_2 | g6077 | g6077.t1 | cds | g6077.t1.CDS4 | 14024292 | 14024890 |
| chr_2 | g6077 | g6077.t1 | TSS | g6077.t1 | NA | NA |
| chr_2 | g6077 | g6077.t1 | TTS | g6077.t1 | NA | NA |
Sequences
>g6077.t1 Gene=g6077 Length=1458
ATGATTTTGTCAATACTCGTGATTTTTTCTATCGTTTTTTACCTCAATTATCGATGGAAA
AGAAGAAAAGTTTATAAAATTGCAGATGCATTACCAGGTTCAAATGGCTATCCAATTTTC
GGTCATATGTTCAGTTTTTTGCGTATGAATTATGAAGATAATTTAAACGAAGCAATGAGA
ATTATTCCGCGAAATCTTCCAATTGCAAAAGTTTGGACTGGAAACGTTCTATTCGTGGTT
ATTGAATCACCAGAACTTTCTCATCAACTTCTAAATTCATCGTCATGTTTGAAAATTCCT
TTCATGTTCTCGCGACCATTTTTAGCAAGATACGCGCTACCAATTTGCAATGGTGAAGTT
TGGCAAAGACATCGAAAAATTCTCAGTCATTCATTTAAAGTTCAAGTTTTGAATGATTTA
ATGCCAACAATAAATGAAAAGTGTCAAAGATTTGTAGCTAAAATGAAGTCACAAATTGGT
TGTGGTGAATTTAATATTTTACATCATATTGCTGCACTTGCTATGGAGACAACAATGAAA
GGAAGTTTTTATTATGATAAAGATTTTTATGGAAATAAATTAATCGATGATATGGACAGA
GCAAAAATTTTTATGATAAAACGAATGGCTCGACCATGGCTTAATTTTGAGTTTTTCTTT
CAACGTTCACAACTTTATCAAGATATTAAAAAAAGTTTCGCGCTCATTCATCAACATGTT
GATGAAATTATAAAACTATATGAAACAAATCGAGCGAATGGTTTTGTTAGTGGAAAAATG
AATATCATTGACCAGCTGATGAGCGAAAAAAGCAATCTCACAAAGGATGAGATAAGAGAT
GAAATTTACATATTTTTATTTGCTGGGTACGAGACTTCATCGATGGCTCTTGCTGCTGTT
TTTCTGCTTATTGCAATTCATAAAGATGTTCAAGCGAAAGTTATTGAAGAAGTTGACGAG
CTTTTTGACGGTGAAAATGTGTCAATGGAAAATTTAAGCAAATTTAAATATGTGGATTAT
GTAGTGAAAGAAACACTTCGACTTTTTCCAACAATTCCTATTGTGCCAAGAAAAGTGACT
GAAGATTTTCAATTGAACGAGTACACAATTCCAAAAGATACAATTCTTCTGGAATTTATT
TATGCTCTTCATCGAAATCCACAAGTATGGGGTGAAGATGTTCTTGAATTCAAACCCGAG
AGATTTGCACCTGAAAATTATCAGAAAATTGATCCTAATGCATTTGTGCCATTTGCTGGT
GGAAGAAGAAAGTGTATAGGCTATAAATATGCAATGAATTTTTTAAAAATTGTAGTCATT
CACTTCTTCAAATATTATGAAGTGAGTTCGTCTCTAAAATATAAAGATTTGACAGCAAAA
ATGACAGTTACACTCGAAATTGCACAGAAATACATGGTTAGTGTTCAACATCGAAAGAAA
AATGTGCCTGCCATTTAA
>g6077.t1 Gene=g6077 Length=485
MILSILVIFSIVFYLNYRWKRRKVYKIADALPGSNGYPIFGHMFSFLRMNYEDNLNEAMR
IIPRNLPIAKVWTGNVLFVVIESPELSHQLLNSSSCLKIPFMFSRPFLARYALPICNGEV
WQRHRKILSHSFKVQVLNDLMPTINEKCQRFVAKMKSQIGCGEFNILHHIAALAMETTMK
GSFYYDKDFYGNKLIDDMDRAKIFMIKRMARPWLNFEFFFQRSQLYQDIKKSFALIHQHV
DEIIKLYETNRANGFVSGKMNIIDQLMSEKSNLTKDEIRDEIYIFLFAGYETSSMALAAV
FLLIAIHKDVQAKVIEEVDELFDGENVSMENLSKFKYVDYVVKETLRLFPTIPIVPRKVT
EDFQLNEYTIPKDTILLEFIYALHRNPQVWGEDVLEFKPERFAPENYQKIDPNAFVPFAG
GRRKCIGYKYAMNFLKIVVIHFFKYYEVSSSLKYKDLTAKMTVTLEIAQKYMVSVQHRKK
NVPAI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g6077.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 31 | 482 | 1.1E-96 |
| 2 | g6077.t1 | PANTHER | PTHR24291:SF145 | CYTOCHROME P450 4AA1-RELATED | 11 | 466 | 5.7E-78 |
| 3 | g6077.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 11 | 466 | 5.7E-78 |
| 5 | g6077.t1 | PRINTS | PR00463 | E-class P450 group I signature | 277 | 294 | 4.0E-10 |
| 12 | g6077.t1 | PRINTS | PR00385 | P450 superfamily signature | 288 | 305 | 6.1E-9 |
| 6 | g6077.t1 | PRINTS | PR00463 | E-class P450 group I signature | 297 | 323 | 4.0E-10 |
| 8 | g6077.t1 | PRINTS | PR00463 | E-class P450 group I signature | 339 | 357 | 4.0E-10 |
| 9 | g6077.t1 | PRINTS | PR00385 | P450 superfamily signature | 340 | 351 | 6.1E-9 |
| 7 | g6077.t1 | PRINTS | PR00463 | E-class P450 group I signature | 415 | 425 | 4.0E-10 |
| 10 | g6077.t1 | PRINTS | PR00385 | P450 superfamily signature | 416 | 425 | 6.1E-9 |
| 4 | g6077.t1 | PRINTS | PR00463 | E-class P450 group I signature | 425 | 448 | 4.0E-10 |
| 11 | g6077.t1 | PRINTS | PR00385 | P450 superfamily signature | 425 | 436 | 6.1E-9 |
| 1 | g6077.t1 | Pfam | PF00067 | Cytochrome P450 | 32 | 450 | 1.4E-76 |
| 17 | g6077.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 18 | g6077.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 1 | - |
| 19 | g6077.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 2 | 13 | - |
| 21 | g6077.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 16 | g6077.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 281 | - |
| 20 | g6077.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 282 | 306 | - |
| 15 | g6077.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 307 | 485 | - |
| 13 | g6077.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 32 | 478 | 3.67E-96 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed