General information for g6082.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Probable cytochrome P450 313a3.
Parent gene
- The parent gene g6082 is located on chr_2: 14040838..14042703 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6082 | g6082.t1 | isoform | g6082.t1 | 14040838 | 14042703 |
| chr_2 | g6082 | g6082.t1 | exon | g6082.t1.exon1 | 14040838 | 14041665 |
| chr_2 | g6082 | g6082.t1 | cds | g6082.t1.CDS1 | 14040838 | 14041665 |
| chr_2 | g6082 | g6082.t1 | exon | g6082.t1.exon2 | 14041767 | 14041785 |
| chr_2 | g6082 | g6082.t1 | cds | g6082.t1.CDS2 | 14041767 | 14041785 |
| chr_2 | g6082 | g6082.t1 | exon | g6082.t1.exon3 | 14042083 | 14042457 |
| chr_2 | g6082 | g6082.t1 | cds | g6082.t1.CDS3 | 14042083 | 14042457 |
| chr_2 | g6082 | g6082.t1 | exon | g6082.t1.exon4 | 14042519 | 14042703 |
| chr_2 | g6082 | g6082.t1 | cds | g6082.t1.CDS4 | 14042519 | 14042703 |
| chr_2 | g6082 | g6082.t1 | TSS | g6082.t1 | NA | NA |
| chr_2 | g6082 | g6082.t1 | TTS | g6082.t1 | NA | NA |
Sequences
>g6082.t1 Gene=g6082 Length=1407
ATGTTTTACATTAACTTCCGGTGGAAGAGAAGAAAGTTATACGCACTAGCTAAAAAAATT
CCTGGGACTGATGGACTTCCACTTATCGGTCACGCGTTCACTTTTATGGGCATCGATGTT
AGAAATAATATAAATGATAGTGGAAAATTACTTCAAAATAATTTACCAGTGTCAAAAATT
TGGGCTGGTTCTTTACTCTTCATTGTTGTCAACTCACCAGATCATTCACAAGCAATGCTT
CAATCAACTGCCTGTCTTAAAATTCCTTTCATTATTTCACGTCCTTTTCTTCATCGCTAT
GGCTTAATTATTGCTAATGGAGCTGTTTGGCAACATCATCGAAAAATTCTTAGTCATTCA
TTTAAAATCAACATTCTTCAACAACTCATGCCAATTTTTAATCAAAAATGTATGAAATTT
GTGGAAAAATTGCAACCAAAAATTGGAGCACCAGAATTCGATATTGGTCACTATGTGGCT
GCTTTGACTTTAGAGACAACTATGACAGGAAATTTTCATTATGATCATTGTTTTTATGGC
AGTAAAATTATATCAGATATTGACCATGGAAAAACATTGATGATGAAAAGAATGATTGAG
CCATGGTGGAATTTTGAATGGCTTTTCCAAAGATCAAAACTTTACAAAGACATGAAAGCA
AGTATCACATTATTAGATGCAAAAATTGATGAAATTATTGAAGCTTATAAAACAAATATG
AACAATAATGAACATAGAGGAACTTTTGTTATTGATCAACTTATGGATCCCGCAAATAAT
CTCACATATGAGGAAATACGCGATGAAATTTTCATTTTCATCATTGCTGGATACGAGACT
TCATCTGTCACTTTGTCAGCAATTCTTCTTATGATTGCAATGCATAAGGATGTTCAAAGA
AAAATGTTTGAAGAAGTCGATGAAAATTATGATGGTGGACATATTGAAATGGAAGATTTA
CATAAATTTAAATATATAGAAATGGTCATTAAAGAAGCTTTACGACTATTTCCAACAGTT
CCTTTTGTGCCTAGAAAAGTCACACAAGAATTTCAAATGAGAGAGTACACAATTCCAAAA
GACACAATTTTAATTGATTTTCTTTACTTTATGCATCGAAATAAGACATATTGGGGAGAA
GATGCTGACAAATTTAGACCAGAACGTTTTGAACCTGAAGAAATTAAGAAAATTCATCCA
CATGCTTACGCTCCATTTGCTGCTGGTAAAAGAGTTTGTATAGGCAACAAATATGCAATG
TATTTTCTTAAAATTGCTCTTGTTCACATTTTCAAACATTATGAGTTCAGCACAACATAC
AAATATGAAGAAATCACTGCAACGATGTCGCCAAGTCTTGAAATTGCTCAAAAATATCAT
GTTTCTGTAGAAAGACGAAAGTCATAA
>g6082.t1 Gene=g6082 Length=468
MFYINFRWKRRKLYALAKKIPGTDGLPLIGHAFTFMGIDVRNNINDSGKLLQNNLPVSKI
WAGSLLFIVVNSPDHSQAMLQSTACLKIPFIISRPFLHRYGLIIANGAVWQHHRKILSHS
FKINILQQLMPIFNQKCMKFVEKLQPKIGAPEFDIGHYVAALTLETTMTGNFHYDHCFYG
SKIISDIDHGKTLMMKRMIEPWWNFEWLFQRSKLYKDMKASITLLDAKIDEIIEAYKTNM
NNNEHRGTFVIDQLMDPANNLTYEEIRDEIFIFIIAGYETSSVTLSAILLMIAMHKDVQR
KMFEEVDENYDGGHIEMEDLHKFKYIEMVIKEALRLFPTVPFVPRKVTQEFQMREYTIPK
DTILIDFLYFMHRNKTYWGEDADKFRPERFEPEEIKKIHPHAYAPFAAGKRVCIGNKYAM
YFLKIALVHIFKHYEFSTTYKYEEITATMSPSLEIAQKYHVSVERRKS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g6082.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 19 | 465 | 3.1E-97 |
| 2 | g6082.t1 | PANTHER | PTHR24291:SF140 | CYTOCHROME P450 4C3-RELATED | 4 | 447 | 2.9E-77 |
| 3 | g6082.t1 | PANTHER | PTHR24291 | CYTOCHROME P450 FAMILY 4 | 4 | 447 | 2.9E-77 |
| 6 | g6082.t1 | PRINTS | PR00463 | E-class P450 group I signature | 265 | 282 | 3.2E-12 |
| 11 | g6082.t1 | PRINTS | PR00385 | P450 superfamily signature | 276 | 293 | 2.3E-10 |
| 8 | g6082.t1 | PRINTS | PR00463 | E-class P450 group I signature | 285 | 311 | 3.2E-12 |
| 5 | g6082.t1 | PRINTS | PR00463 | E-class P450 group I signature | 327 | 345 | 3.2E-12 |
| 10 | g6082.t1 | PRINTS | PR00385 | P450 superfamily signature | 328 | 339 | 2.3E-10 |
| 7 | g6082.t1 | PRINTS | PR00463 | E-class P450 group I signature | 403 | 413 | 3.2E-12 |
| 9 | g6082.t1 | PRINTS | PR00385 | P450 superfamily signature | 404 | 413 | 2.3E-10 |
| 4 | g6082.t1 | PRINTS | PR00463 | E-class P450 group I signature | 413 | 436 | 3.2E-12 |
| 12 | g6082.t1 | PRINTS | PR00385 | P450 superfamily signature | 413 | 424 | 2.3E-10 |
| 1 | g6082.t1 | Pfam | PF00067 | Cytochrome P450 | 21 | 440 | 1.9E-80 |
| 17 | g6082.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 269 | - |
| 18 | g6082.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 270 | 293 | - |
| 16 | g6082.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 294 | 468 | - |
| 14 | g6082.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 20 | 466 | 4.32E-97 |
| 13 | g6082.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 270 | 292 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed