General information for g6087.t2
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Serine protease ami.
Parent gene
- The parent gene g6087 is located on chr_2: 14060075..14061050 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6087 | g6087.t2 | TSS | g6087.t2 | 14059276 | 14059276 |
| chr_2 | g6087 | g6087.t2 | isoform | g6087.t2 | 14059324 | 14060386 |
| chr_2 | g6087 | g6087.t2 | exon | g6087.t2.exon1 | 14059324 | 14059369 |
| chr_2 | g6087 | g6087.t2 | cds | g6087.t2.CDS1 | 14059324 | 14059369 |
| chr_2 | g6087 | g6087.t2 | exon | g6087.t2.exon2 | 14059422 | 14059768 |
| chr_2 | g6087 | g6087.t2 | cds | g6087.t2.CDS2 | 14059422 | 14059768 |
| chr_2 | g6087 | g6087.t2 | exon | g6087.t2.exon3 | 14059829 | 14060078 |
| chr_2 | g6087 | g6087.t2 | cds | g6087.t2.CDS3 | 14059829 | 14060078 |
| chr_2 | g6087 | g6087.t2 | exon | g6087.t2.exon4 | 14060160 | 14060386 |
| chr_2 | g6087 | g6087.t2 | cds | g6087.t2.CDS4 | 14060160 | 14060386 |
| chr_2 | g6087 | g6087.t2 | TTS | g6087.t2 | 14060418 | 14060418 |
Sequences
>g6087.t2 Gene=g6087 Length=870
ATGTTATGGATTACAAATTTAATACTTTTTGCATCGATTTTAAATTGTCATGGTTATGAA
AATATTGAGGGAAGAGTTGTGAATGGAGTGACAGCAAAGAATATTATACCATATCAAGCG
TCGATAAGAGTTTCTGTTAGAGATTATTTGCGCTTTGGACGAGGTCATACATGTGGAGGA
GTGTTGATATCTCGTCGAACTGTTACAACAGCTGCTCACTGTTTAACGGATGGTGCTAGA
ATGAGAACAGTCTTTGATATCCATGTTGTTTTTGGATCACTTAATCGCTACATTTTTACT
GACGAGACTGTGATAATGCATGCTGAAAAAATTATCATGCATCCCAAATATAGGAGATTT
GAAAGCTTGGAACATGACATTGGACTTGTTATTCTCAGACGAAATGTAGAGTTAAGTGAC
ATAATTCATCCGATTCCATTGATAGATTTTGCAATACCAACAGGCAATAGATGCCAAATA
AGTGGTTGGGGTGCAACACAATGGAGAGGTCTGATGCCTTTACAATTGCAAAAGGCAAAT
GTTTCAATTGTCGATAGAAATTTTTGTAATGCTTCATATTCGGAGCGTTTTGGCACTTCT
ATTACATATGGTATGGTATGTGCAAATGGTCAAACAGAATCTGATTCTGGTGGACCACTT
CAGTGTGATGACAAACTTGTAGGACTTTCGTCATTTGGCGTATCATGTGGTGCATCATTC
GATATTCCTGGCGTTTATGTCGACATTTATTATTACCACAATTGGATTATGGAAAATTGG
AAGAACAATGGTGTGATGCAAAAAAGTCACAAAACCTCAATTTTCATGTTCACACTTTTT
GTAAATTTAATTTGGAATTTGAAGTCATAA
>g6087.t2 Gene=g6087 Length=289
MLWITNLILFASILNCHGYENIEGRVVNGVTAKNIIPYQASIRVSVRDYLRFGRGHTCGG
VLISRRTVTTAAHCLTDGARMRTVFDIHVVFGSLNRYIFTDETVIMHAEKIIMHPKYRRF
ESLEHDIGLVILRRNVELSDIIHPIPLIDFAIPTGNRCQISGWGATQWRGLMPLQLQKAN
VSIVDRNFCNASYSERFGTSITYGMVCANGQTESDSGGPLQCDDKLVGLSSFGVSCGASF
DIPGVYVDIYYYHNWIMENWKNNGVMQKSHKTSIFMFTLFVNLIWNLKS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g6087.t2 | CDD | cd00190 | Tryp_SPc | 26 | 259 | 7.6224E-60 |
| 9 | g6087.t2 | Gene3D | G3DSA:2.40.10.10 | - | 18 | 262 | 1.0E-54 |
| 2 | g6087.t2 | PANTHER | PTHR24276 | POLYSERASE-RELATED | 10 | 261 | 2.9E-47 |
| 3 | g6087.t2 | PANTHER | PTHR24276:SF78 | AT20289P-RELATED | 10 | 261 | 2.9E-47 |
| 6 | g6087.t2 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 59 | 74 | 1.0E-7 |
| 5 | g6087.t2 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 122 | 136 | 1.0E-7 |
| 4 | g6087.t2 | PRINTS | PR00722 | Chymotrypsin serine protease family (S1) signature | 209 | 221 | 1.0E-7 |
| 1 | g6087.t2 | Pfam | PF00089 | Trypsin | 33 | 256 | 6.4E-45 |
| 11 | g6087.t2 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 12 | g6087.t2 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 6 | - |
| 13 | g6087.t2 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 7 | 14 | - |
| 14 | g6087.t2 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 15 | 18 | - |
| 10 | g6087.t2 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 289 | - |
| 17 | g6087.t2 | ProSiteProfiles | PS50240 | Serine proteases, trypsin domain profile. | 26 | 261 | 25.247 |
| 16 | g6087.t2 | SMART | SM00020 | trypsin_2 | 25 | 256 | 6.6E-53 |
| 7 | g6087.t2 | SUPERFAMILY | SSF50494 | Trypsin-like serine proteases | 19 | 262 | 4.66E-62 |
| 8 | g6087.t2 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004252 | serine-type endopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed