Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative 60S ribosomal protein L28.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6215 g6215.t18 TTS g6215.t18 15291317 15291317
chr_2 g6215 g6215.t18 isoform g6215.t18 15291398 15292626
chr_2 g6215 g6215.t18 exon g6215.t18.exon1 15291398 15291598
chr_2 g6215 g6215.t18 cds g6215.t18.CDS1 15291398 15291598
chr_2 g6215 g6215.t18 exon g6215.t18.exon2 15291781 15291924
chr_2 g6215 g6215.t18 cds g6215.t18.CDS2 15291781 15291894
chr_2 g6215 g6215.t18 exon g6215.t18.exon3 15292095 15292192
chr_2 g6215 g6215.t18 exon g6215.t18.exon4 15292574 15292626
chr_2 g6215 g6215.t18 TSS g6215.t18 15292596 15292596

Sequences

>g6215.t18 Gene=g6215 Length=496
ATGATGCAGAGAAAAAATGATGCGAACTTTCTTTTCGTTCCATCTGTCAAAATAGAGAAA
AATGGCCTCGTCGCATCTCAATTGGCTGATTGTTCGCGATAACAACGCTTTTATTTTAAA
GCAACGTAATATCCGCAAGCCATTCAGCAAGTTTGTTCATTTTTAGGAACCAAACAATTT
GATGAATGTCAGCACATTCCGCTATAGTGGATTAGTTCATCCACACGCGATTGGTATCAC
ACCATCAGTTGACAAGAAGGGAATCATCTTTTCATACAAAAAACCCAAAAAATTGTATAA
ACCAGCTGAAAGCATTGGAAGAATCACCTTCAAATCGGGCCAGAAGCGTACATTGAAGAA
AGTTAAGAACGTCATTCAATCAAAGAAATACCGTCGTGATTTACAACAGGCTGCTTTGAG
ACGTGTCAGTGCTATCTATCGCTCACAGAAAACAAAGCCCGCTAAAAAGAAGGGCGGCGC
AAAGAAAGCCGAATAA

>g6215.t18 Gene=g6215 Length=104
MNVSTFRYSGLVHPHAIGITPSVDKKGIIFSYKKPKKLYKPAESIGRITFKSGQKRTLKK
VKNVIQSKKYRRDLQQAALRRVSAIYRSQKTKPAKKKGGAKKAE

Protein features from InterProScan

Transcript Database ID Name Start End E.value
6 g6215.t18 Gene3D G3DSA:3.30.390.110 - 1 93 4.5E-28
4 g6215.t18 MobiDBLite mobidb-lite consensus disorder prediction 85 104 -
5 g6215.t18 MobiDBLite mobidb-lite consensus disorder prediction 90 104 -
2 g6215.t18 PANTHER PTHR10544:SF0 60S RIBOSOMAL PROTEIN L28 2 97 3.4E-22
3 g6215.t18 PANTHER PTHR10544 60S RIBOSOMAL PROTEIN L28 2 97 3.4E-22
1 g6215.t18 Pfam PF01778 Ribosomal L28e protein family 2 88 7.5E-18

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005840 ribosome CC
GO:0006412 translation BP
GO:0003735 structural constituent of ribosome MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values