General information for g6216.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Protein windbeutel.
Parent gene
- The parent gene g6216 is located on chr_2: 15293891..15297365 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6216 | g6216.t3 | TSS | g6216.t3 | 15292861 | 15292861 |
| chr_2 | g6216 | g6216.t3 | isoform | g6216.t3 | 15292958 | 15293891 |
| chr_2 | g6216 | g6216.t3 | exon | g6216.t3.exon1 | 15292958 | 15293079 |
| chr_2 | g6216 | g6216.t3 | cds | g6216.t3.CDS1 | 15292958 | 15293079 |
| chr_2 | g6216 | g6216.t3 | exon | g6216.t3.exon2 | 15293140 | 15293396 |
| chr_2 | g6216 | g6216.t3 | cds | g6216.t3.CDS2 | 15293140 | 15293396 |
| chr_2 | g6216 | g6216.t3 | exon | g6216.t3.exon3 | 15293455 | 15293618 |
| chr_2 | g6216 | g6216.t3 | cds | g6216.t3.CDS3 | 15293455 | 15293618 |
| chr_2 | g6216 | g6216.t3 | exon | g6216.t3.exon4 | 15293685 | 15293891 |
| chr_2 | g6216 | g6216.t3 | cds | g6216.t3.CDS4 | 15293685 | 15293891 |
| chr_2 | g6216 | g6216.t3 | TTS | g6216.t3 | NA | NA |
Sequences
>g6216.t3 Gene=g6216 Length=750
ATGCAGTTATTTACAATTATCTTGTCATTTTCATTGATCAATTTGAGTTTAGCATGTTCC
GGCTGCATAGATTTAGATGAATTGACTTTTGAAAAAATTTTAAGTAAATTTAAAACAGCA
TTGGTAAAATTTGATCAATCATTTCCATATGGAGAAAATCATGAAGCTTTTGCTGCTTTT
TCTCAACAAATTAACAATTTGACATACACAGAGACTACAAATCATCCTGATGTCTTAATT
GCAACAGTTGGTGTTAAAGACTATGGTGATATGGAAAATAAAAAGTTAGCAGACAAATAT
GGAGTCAGTGTTGAAGCATTTCCAGCCATCAAGCTATTTAATGATGGAAATTTGGAACAA
CCCATAAGTTTTGATTTAGGAAAAGAGAAATTAACTGTGAGCAAATTGTTTCTATTTTTG
AAAGAGCATGCATCAGCTTATGTTAAAGCACCAGGTTGTAATGAAGAACTTGATGAATTG
TCTAAGCAATTTATGGCTAAAAAAAGTGATAAGAATAGTATTTTAAAGAAAGCAAAGGCT
ATTAAAAAGAGTATAGCTAAATTTTATGTAACATTAATGAAGAACTCAATCGAAAAGGGT
AAAGAATTCATTGCACTTCAACAAGAACGTATGACGAAACTTTTGGGAGATAAGATAAGT
GAGAAAAAGTCTGAAGAAATTAACAAGAAACTGAATATTTTAATGTCGTTTAAAAAATAT
GATCAAGAGAATAAGAAGGAGGAGTTATAA
>g6216.t3 Gene=g6216 Length=249
MQLFTIILSFSLINLSLACSGCIDLDELTFEKILSKFKTALVKFDQSFPYGENHEAFAAF
SQQINNLTYTETTNHPDVLIATVGVKDYGDMENKKLADKYGVSVEAFPAIKLFNDGNLEQ
PISFDLGKEKLTVSKLFLFLKEHASAYVKAPGCNEELDELSKQFMAKKSDKNSILKKAKA
IKKSIAKFYVTLMKNSIEKGKEFIALQQERMTKLLGDKISEKKSEEINKKLNILMSFKKY
DQENKKEEL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g6216.t3 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 10 | 149 | 4.0E-33 |
| 9 | g6216.t3 | Gene3D | G3DSA:1.20.1150.12 | - | 151 | 249 | 1.0E-20 |
| 4 | g6216.t3 | PANTHER | PTHR12211:SF0 | ENDOPLASMIC RETICULUM RESIDENT PROTEIN 29 | 6 | 245 | 3.3E-56 |
| 5 | g6216.t3 | PANTHER | PTHR12211 | ENDOPLASMIC RETICULUM PROTEIN ERP29 | 6 | 245 | 3.3E-56 |
| 3 | g6216.t3 | Pfam | PF07912 | ERp29, N-terminal domain | 18 | 149 | 1.4E-36 |
| 2 | g6216.t3 | Pfam | PF07749 | Endoplasmic reticulum protein ERp29, C-terminal domain | 152 | 237 | 4.4E-14 |
| 12 | g6216.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 13 | g6216.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 14 | g6216.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 13 | - |
| 15 | g6216.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 11 | g6216.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 249 | - |
| 6 | g6216.t3 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 20 | 142 | 5.71E-13 |
| 7 | g6216.t3 | SUPERFAMILY | SSF47933 | ERP29 C domain-like | 151 | 242 | 6.02E-16 |
| 8 | g6216.t3 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
| 1 | g6216.t3 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005788 | endoplasmic reticulum lumen | CC |
| GO:0009306 | protein secretion | BP |
| GO:0005783 | endoplasmic reticulum | CC |
KEGG
Orthology
- This transcript has been annotated with K09586 (endoplasmic reticulum protein 29)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed