General information for g6255.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Probable dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase.
Parent gene
- The parent gene g6255 is located on chr_2: 15632891..15634824 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6255 | g6255.t1 | TSS | g6255.t1 | 15632646 | 15632646 |
| chr_2 | g6255 | g6255.t1 | isoform | g6255.t1 | 15632891 | 15634824 |
| chr_2 | g6255 | g6255.t1 | exon | g6255.t1.exon1 | 15632891 | 15633591 |
| chr_2 | g6255 | g6255.t1 | cds | g6255.t1.CDS1 | 15632891 | 15633591 |
| chr_2 | g6255 | g6255.t1 | exon | g6255.t1.exon2 | 15633644 | 15633847 |
| chr_2 | g6255 | g6255.t1 | cds | g6255.t1.CDS2 | 15633644 | 15633847 |
| chr_2 | g6255 | g6255.t1 | exon | g6255.t1.exon3 | 15633901 | 15634375 |
| chr_2 | g6255 | g6255.t1 | cds | g6255.t1.CDS3 | 15633901 | 15634375 |
| chr_2 | g6255 | g6255.t1 | exon | g6255.t1.exon4 | 15634630 | 15634824 |
| chr_2 | g6255 | g6255.t1 | cds | g6255.t1.CDS4 | 15634630 | 15634824 |
| chr_2 | g6255 | g6255.t1 | TTS | g6255.t1 | 15634996 | 15634996 |
Sequences
>g6255.t1 Gene=g6255 Length=1575
ATGACATTCTACAAATATGCAATTATTGTATGTGCTGGAATATTCTTAAGAACTACAATT
TCGTTACATTCTTATTCGGGACAAGGTAAAAAGCCGCTTTTTGGAGATTTTGAAGCACAA
CGGCATTGGCAAGAAGTTACAGTGAATTTACCCATAAAAGATTGGTATGAAAACACTACA
GATAATGATTTGTTATATTGGGGACTAGACTACCCACCATTAACTGCATATCACAGTTAT
TTAATTGGACAAACTGCGAAATTCGTCAATGAATCTTTTGTTGAACTTCACAAATCGAGA
GGAATTGAAACTGAAGAGCATAAGCAGTTTATGAGAATGACTGTACTTATTGCTGATATT
CTAATCTATATCCCTGCTATGCTATTGGCTTGCAATGTGATATTCTTTAGAATTTTAAAA
ATTAATGCTGAAAAAGGTGAAAGCTTTCATTGGCTTTATTTATGCATTGCCATTCTATAT
CCTGGACAAATTTTGATTGATAATGGACACTTTCAATACAATAATATTTCATTAGGATTC
ACTGTGATTGCTATTTCTGCAATCTTATGTGATCAACGACTTATTGGAGCAATATTCTTT
GTTTTCGCCTTAAATTACAAACAAATGGAGTTGTATCATGCTTTGCCTTTCTTTTGCTAT
CTTTTATCAACTTGTTTTATTGACCGTGAAAGACAAAAGAGTTTCACATTTATTACGGGA
ATCAAGAGACTTTCATTGCTTGGAATAGTTGTTGTGATTTCATTTCTCTTGATCTGGCTT
CCATGGTTGTCCTCACTCGAAAGTTTACTTCAAGTTGTTCATCGAATTTTTCCAATATAT
CGAGGAGTTTTTGAAGACAAAGTGTCAAATATTTGGTGCATTGTCAATGTTTTTATGAAA
ATCAAAGAAAATTTCACAAATGAGCAAATGGCAAAAATCTGCTTAATGTGCACAGCTACA
TCAATCGTTCCGAGTTCAATAACACTTCTGTTAAAACCAACAAAACGACAATTTCTTTAT
TCACTCATCAATTGTTCTCTTGCTTTCTTTCTCTTCTCGTTTCAAGTTCATGAGAAATCA
ATTTTAATTCCTGCTATTTCAGTTGCTTTGATCTTCTCACTTGAGCCATTCATGTCATTT
TGGTTTCTTCAAATTTCAACATTCAGCATGCTTCCATTATTGGTTAAAGATGGATTATTT
GCTGCATTTATTGGACTTTCTGGATTTACAATTCTTTTAGTTAAATTAATCATTGATGAA
TCGAAAGATCAGAAAAATAAGAATTCAAATTATTTACAAATTTTGTTGAAGATAAATAAT
TTAACAAGTAGAAAATGGCTTGAGAATTTCATGATATTCAGCTATCTGTTGTCGACAGTT
GAAGTTGAAGACTTAAGTGACGGATGTGGAGGAAAATTTGCTGCAATCATTGTTTCCGAT
CAATTTGCTGGTAAATCTTTGCTCGCACGACATCGCCTCGTTAATACAGCATTAGCTGAA
GAACTCAAAGAAATTCACGCATTTTCACAAAAGACATTCACACCAGAACAGTGGCAAGAA
CAACAGAATCAATAA
>g6255.t1 Gene=g6255 Length=524
MTFYKYAIIVCAGIFLRTTISLHSYSGQGKKPLFGDFEAQRHWQEVTVNLPIKDWYENTT
DNDLLYWGLDYPPLTAYHSYLIGQTAKFVNESFVELHKSRGIETEEHKQFMRMTVLIADI
LIYIPAMLLACNVIFFRILKINAEKGESFHWLYLCIAILYPGQILIDNGHFQYNNISLGF
TVIAISAILCDQRLIGAIFFVFALNYKQMELYHALPFFCYLLSTCFIDRERQKSFTFITG
IKRLSLLGIVVVISFLLIWLPWLSSLESLLQVVHRIFPIYRGVFEDKVSNIWCIVNVFMK
IKENFTNEQMAKICLMCTATSIVPSSITLLLKPTKRQFLYSLINCSLAFFLFSFQVHEKS
ILIPAISVALIFSLEPFMSFWFLQISTFSMLPLLVKDGLFAAFIGLSGFTILLVKLIIDE
SKDQKNKNSNYLQILLKINNLTSRKWLENFMIFSYLLSTVEVEDLSDGCGGKFAAIIVSD
QFAGKSLLARHRLVNTALAEELKEIHAFSQKTFTPEQWQEQQNQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g6255.t1 | Gene3D | G3DSA:3.10.20.90 | - | 441 | 524 | 1.4E-27 |
| 3 | g6255.t1 | PANTHER | PTHR12413:SF1 | DOLICHYL PYROPHOSPHATE MAN9GLCNAC2 ALPHA-1,3-GLUCOSYLTRANSFERASE | 6 | 435 | 5.2E-156 |
| 4 | g6255.t1 | PANTHER | PTHR12413 | DOLICHYL GLYCOSYLTRANSFERASE | 6 | 435 | 5.2E-156 |
| 1 | g6255.t1 | Pfam | PF03155 | ALG6, ALG8 glycosyltransferase family | 18 | 450 | 5.0E-133 |
| 2 | g6255.t1 | Pfam | PF01722 | BolA-like protein | 467 | 517 | 3.2E-16 |
| 22 | g6255.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 26 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 25 | - |
| 18 | g6255.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 26 | 114 | - |
| 31 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 115 | 136 | - |
| 24 | g6255.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 137 | 147 | - |
| 32 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 148 | 166 | - |
| 19 | g6255.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 167 | 177 | - |
| 28 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 178 | 205 | - |
| 21 | g6255.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 206 | 210 | - |
| 27 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 211 | 228 | - |
| 15 | g6255.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 229 | 239 | - |
| 34 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 240 | 259 | - |
| 20 | g6255.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 260 | 278 | - |
| 35 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 279 | 298 | - |
| 14 | g6255.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 299 | 309 | - |
| 30 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 310 | 331 | - |
| 23 | g6255.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 332 | 336 | - |
| 33 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 337 | 354 | - |
| 17 | g6255.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 355 | 360 | - |
| 29 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 361 | 383 | - |
| 25 | g6255.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 384 | 397 | - |
| 36 | g6255.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 398 | 418 | - |
| 16 | g6255.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 419 | 524 | - |
| 12 | g6255.t1 | SUPERFAMILY | SSF82657 | BolA-like | 457 | 523 | 5.49E-25 |
| 6 | g6255.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 114 | 136 | - |
| 9 | g6255.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 149 | 166 | - |
| 5 | g6255.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 181 | 203 | - |
| 8 | g6255.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 244 | 263 | - |
| 7 | g6255.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 337 | 354 | - |
| 11 | g6255.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 361 | 383 | - |
| 10 | g6255.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 398 | 417 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005783 | endoplasmic reticulum | CC |
| GO:0042281 | dolichyl pyrophosphate Man9GlcNAc2 alpha-1,3-glucosyltransferase activity | MF |
| GO:0016758 | hexosyltransferase activity | MF |
| GO:0006488 | dolichol-linked oligosaccharide biosynthetic process | BP |
KEGG
Orthology
- This transcript has been annotated with K03848 (alpha-1,3-glucosyltransferase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
