Gene loci information

Transcript annotation

  • This transcript has been annotated as NADH-cytochrome b5 reductase 3 .

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6286 g6286.t5 TSS g6286.t5 15837834 15837834
chr_2 g6286 g6286.t5 isoform g6286.t5 15837970 15840228
chr_2 g6286 g6286.t5 exon g6286.t5.exon1 15837970 15837984
chr_2 g6286 g6286.t5 exon g6286.t5.exon2 15838419 15838546
chr_2 g6286 g6286.t5 exon g6286.t5.exon3 15838922 15840228
chr_2 g6286 g6286.t5 cds g6286.t5.CDS1 15839219 15839749
chr_2 g6286 g6286.t5 TTS g6286.t5 NA NA

Sequences

>g6286.t5 Gene=g6286 Length=1450
ATGAGTGATTTCGATGCATTTCCATTCATCACCGGTGCTATTACAATTTTAGTGACTGTC
TTGCTGTATAAAGTATTTAAAGCAAAGCAATTACACAAAGACGATGAAGAGGAGGAAGAT
GAACAACAGGATCAAGTAGCGAGAAGCCTGTTGGACCGCTGAAGACACTCGTCGAACCGA
ATGAGAAATATTTTTTGCCTTTAGTTGAAAAAGAAGAAATTAATCATGATACAAGAAAAT
TCCGTTTTGCATTGCCCAGTCCTGATCACGTATTAGGATTACCTGTCGGTCAACACGTTC
ACTTATCTGTGTCAATAAATAATGAACTCGTTATCAGATCGTATACGCCCGTTTCGAGCG
ATGATGATAAGGGCTTTGTTGACTTAGTTATTAAAGTATATAGAAGAAATATTCATCCAA
AGTTCCCTGATGGTGGAAAAATGTCTCAATATCTTGATGAAATGAAAATTGGCGATACAA
TTGCATTTAGAGGACCATCTGGGAAATTGCAATATCTTGGCAAAGGGAAATTTGCAATTA
AAAAACTTCGTAAGGATCCACCACAAATTATAAATGTACAGAAAGTTAATATGATTGCTG
GTGGAAGTGGAATTACACCAATGCTTCAACTTATCAATGAAATGCTTAAAAAGTCTGACG
ATAACACACAAATTGCTCTTCTTTATGCCAATCAAACAGAGGACGATATTTTGTTGCGCG
ATGATATTGATGTATTGGCTGCAAAACATCAAGAACAATTTAAAGTTTGGTATACAGTAG
ACCGTGCATTAACACCTAATTGGAAATATAGCACTGGTTTTGTTAATGATACCATGATTC
AAGAAAACCTCTTTCCTCCATCATCCGATTGTTTGACAGTCATGTGTGGACCGCCGCCAA
TGATCAATTTTGCTTGCCTGCCTAATCTTGACAAACTTGGTTACGATAAGGAATTGAACT
TTGCTTACTAAAACAAATTAGCGAACAAATATATTCTTCTGCCTTTTAGCAATATTATCA
AACTAGAGACAATGTTTTTTTACGTACATACGTGGTTGTTAATTGAAGATGATGAATGAA
AGACATAATTTTTGCCGGCATTTAGTATTAGTGAGATTAAAGTGTTTCTTTTTTGCCAAT
ATCAATGAGAAGTATTAGAGAGATTGTATCCATTGTGCACTACTTACTTCTTCGAATAAT
TTGTTAAATTCTTTCCTTTGTCATACTGTAAAGTTGTTTTTTGGGGGATAAATTTGAAAT
TTCAATAAAATAGATCAAAACGATCGCATTGTTTTATATTATAAAAAGTCGTTCTATTTG
TCATTTATTTAAAAGAATTTGAGATTCATTCAAAATTGGACGGATCAAATCACTAAGATA
TGAGAATTTATATTGTTAAGAGCACTTTGGAATGTATATGTTGTTTGCTTTTCTAGTTAA
GTTTTTAGAT

>g6286.t5 Gene=g6286 Length=176
MSQYLDEMKIGDTIAFRGPSGKLQYLGKGKFAIKKLRKDPPQIINVQKVNMIAGGSGITP
MLQLINEMLKKSDDNTQIALLYANQTEDDILLRDDIDVLAAKHQEQFKVWYTVDRALTPN
WKYSTGFVNDTMIQENLFPPSSDCLTVMCGPPPMINFACLPNLDKLGYDKELNFAY

Protein features from InterProScan

Transcript Database ID Name Start End E.value
14 g6286.t5 CDD cd06183 cyt_b5_reduct_like 1 176 0e+00
13 g6286.t5 Gene3D G3DSA:3.40.50.80 - 21 176 0e+00
3 g6286.t5 PANTHER PTHR19370 NADH-CYTOCHROME B5 REDUCTASE 1 176 0e+00
4 g6286.t5 PANTHER PTHR19370:SF108 NADH-CYTOCHROME B5 REDUCTASE 2 1 176 0e+00
9 g6286.t5 PRINTS PR00406 Cytochrome B5 reductase signature 11 25 0e+00
6 g6286.t5 PRINTS PR00371 Flavoprotein pyridine nucleotide cytochrome reductase signature 50 69 2e-05
10 g6286.t5 PRINTS PR00406 Cytochrome B5 reductase signature 50 69 0e+00
7 g6286.t5 PRINTS PR00371 Flavoprotein pyridine nucleotide cytochrome reductase signature 88 99 2e-05
11 g6286.t5 PRINTS PR00406 Cytochrome B5 reductase signature 88 99 0e+00
5 g6286.t5 PRINTS PR00371 Flavoprotein pyridine nucleotide cytochrome reductase signature 145 153 2e-05
8 g6286.t5 PRINTS PR00406 Cytochrome B5 reductase signature 145 153 0e+00
1 g6286.t5 Pfam PF00970 Oxidoreductase FAD-binding domain 1 25 5e-05
2 g6286.t5 Pfam PF00175 Oxidoreductase NAD-binding domain 51 159 0e+00
12 g6286.t5 SUPERFAMILY SSF52343 Ferredoxin reductase-like, C-terminal NADP-linked domain 12 171 0e+00

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0055114 NA NA
GO:0016491 oxidoreductase activity MF

KEGG

Orthology

This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values