General information for g6296.t12
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Enolase.
Parent gene
- The parent gene g6296 is located on chr_2: 16019021..16020627 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6296 | g6296.t12 | TTS | g6296.t12 | 16018227 | 16018227 |
| chr_2 | g6296 | g6296.t12 | isoform | g6296.t12 | 16019021 | 16020538 |
| chr_2 | g6296 | g6296.t12 | exon | g6296.t12.exon1 | 16019021 | 16019620 |
| chr_2 | g6296 | g6296.t12 | cds | g6296.t12.CDS1 | 16019023 | 16019613 |
| chr_2 | g6296 | g6296.t12 | exon | g6296.t12.exon2 | 16020485 | 16020538 |
| chr_2 | g6296 | g6296.t12 | TSS | g6296.t12 | 16020625 | 16020625 |
Sequences
>g6296.t12 Gene=g6296 Length=654
AGATTATTTTATTAAAAGTGCAACATTGTGCTATCATTATAACCTGCTCTCATACTCAAA
AATGCCAATCAAAAGCATCAAAGCACGTCAAATTTTTGACTCTCGCGGTAACCCAACCGT
TGAAGTTGATCTCACAACTGAATTGGGAATGTTCCGTGCTGCAGTTCCATCAGGAGCTTC
GACTGGCATCCATGAAGCACTTGAATTACGTGACAAGGAAGCTGCTAACTGGATGGGAAA
GGGTGTTTTGAAAGCCGTTGCTCATGTCAATGACTCACTCGGCCCAGAAGTTATCAAATC
AGGCTTGGAAGTAACTCAACAACGCGAAATCGATGAATTGATGATCAAACTTGATGGCAC
ACCAAACAAATCAAAGTTCGGTGCTAATGCTATTTTGGGTGTCTCATTGGCTGTCTGCAA
AGCTGGTGCAGCAAAGAAAGGTGTTCCTCTTTATCAACATATTGCTGACTTAGCTGGCAA
CAAGGATATCTGGCTTCCAGTTCCAGCTTTTAACGTCATTAACGGTGGTGAACATGCTGG
TAACTTGTTAGCTATGCAAGAATTTATGCTCTTGCCAGTTGGAGCTAAGTCATTCACTGA
GGCAATGAAAATGGGAGCTGAAACATACCACAATTTGGCTAAAGTTATTCAACA
>g6296.t12 Gene=g6296 Length=197
MPIKSIKARQIFDSRGNPTVEVDLTTELGMFRAAVPSGASTGIHEALELRDKEAANWMGK
GVLKAVAHVNDSLGPEVIKSGLEVTQQREIDELMIKLDGTPNKSKFGANAILGVSLAVCK
AGAAKKGVPLYQHIADLAGNKDIWLPVPAFNVINGGEHAGNLLAMQEFMLLPVGAKSFTE
AMKMGAETYHNLAKVIQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g6296.t12 | Gene3D | G3DSA:3.30.390.10 | - | 1 | 127 | 0 |
| 11 | g6296.t12 | Gene3D | G3DSA:3.20.20.120 | Enolase superfamily | 128 | 197 | 0 |
| 3 | g6296.t12 | PANTHER | PTHR11902:SF5 | BETA-ENOLASE | 1 | 196 | 0 |
| 4 | g6296.t12 | PANTHER | PTHR11902 | ENOLASE | 1 | 196 | 0 |
| 5 | g6296.t12 | PRINTS | PR00148 | Enolase signature | 35 | 49 | 0 |
| 6 | g6296.t12 | PRINTS | PR00148 | Enolase signature | 107 | 123 | 0 |
| 7 | g6296.t12 | PRINTS | PR00148 | Enolase signature | 164 | 177 | 0 |
| 1 | g6296.t12 | Pfam | PF03952 | Enolase, N-terminal domain | 3 | 134 | 0 |
| 2 | g6296.t12 | Pfam | PF00113 | Enolase, C-terminal TIM barrel domain | 144 | 196 | 0 |
| 10 | g6296.t12 | SMART | SM01193 | Enolase_N_3 | 3 | 134 | 0 |
| 8 | g6296.t12 | SUPERFAMILY | SSF54826 | Enolase N-terminal domain-like | 3 | 138 | 0 |
| 9 | g6296.t12 | SUPERFAMILY | SSF51604 | Enolase C-terminal domain-like | 143 | 196 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0000287 | magnesium ion binding | MF |
| GO:0004634 | phosphopyruvate hydratase activity | MF |
| GO:0006096 | glycolytic process | BP |
| GO:0000015 | phosphopyruvate hydratase complex | CC |
KEGG
Orthology
- This transcript has been annotated with K01689 (enolase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
