General information for g6296.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Enolase.
Parent gene
- The parent gene g6296 is located on chr_2: 16019021..16020627 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6296 | g6296.t3 | isoform | g6296.t3 | 16017843 | 16020627 |
| chr_2 | g6296 | g6296.t3 | exon | g6296.t3.exon1 | 16017843 | 16019168 |
| chr_2 | g6296 | g6296.t3 | TTS | g6296.t3 | 16017865 | 16017865 |
| chr_2 | g6296 | g6296.t3 | cds | g6296.t3.CDS1 | 16018306 | 16019168 |
| chr_2 | g6296 | g6296.t3 | exon | g6296.t3.exon2 | 16019295 | 16019620 |
| chr_2 | g6296 | g6296.t3 | cds | g6296.t3.CDS2 | 16019295 | 16019613 |
| chr_2 | g6296 | g6296.t3 | exon | g6296.t3.exon3 | 16020562 | 16020627 |
| chr_2 | g6296 | g6296.t3 | TSS | g6296.t3 | 16020625 | 16020625 |
Sequences
>g6296.t3 Gene=g6296 Length=1718
ATTTTCAAGCTGAATCGCGTTGAGAAAGTTCACTTGTAGCCGATTTCCTGAAAATTAATT
GAAAAACTCAAAAATGCCAATCAAAAGCATCAAAGCACGTCAAATTTTTGACTCTCGCGG
TAACCCAACCGTTGAAGTTGATCTCACAACTGAATTGGGAATGTTCCGTGCTGCAGTTCC
ATCAGGAGCTTCGACTGGCATCCATGAAGCACTTGAATTACGTGACAAGGAAGCTGCTAA
CTGGATGGGAAAGGGTGTTTTGAAAGCCGTTGCTCATGTCAATGACTCACTCGGCCCAGA
AGTTATCAAATCAGGCTTGGAAGTAACTCAACAACGCGAAATCGATGAATTGATGATCAA
ACTTGATGGCACACCAAACAAATCAAAGTTCGCTTTTAACGTCATTAACGGTGGTGAACA
TGCTGGTAACTTGTTAGCTATGCAAGAATTTATGCTCTTGCCAGTTGGAGCTAAGTCATT
CACTGAGGCAATGAAAATGGGAGCTGAAACATACCACAATTTGGCTAAAGTTATTCAACA
AAAGTTCGGAAAGGATGCCACAGCTGTCGGTGATGAAGGTGGTTTTGCACCAAACATCCT
TGATAATCGTGCTGGTTTGGAATTGATTGTTGAGGCTATTGAAAAAGCTGGTTACACTGG
CAAAATTAAGATCGGTATGGATGTTGCTGCTTCAGAATTCAAGGACGAAGATGGAACATA
TGACTTATTCAAGAAAGACAAGGTTAAGGATAAGTCACGCAACTTATCATCAGATAAATT
GGGTGATCTCTATATGGAATTCATCAAGGATTTCCCAGTTGTAAGTATTGAGGACCCATT
TGATCAAGACGATTGGGAAGCATGGACTAAACTTAATGCTCGTGCTGGAATTCAAATTGT
CGGTGATGATTTGACTGTCACTAATCCAAAACGTATTCAAACCGCTGTCGAAAAGAAGGC
TTGCAACTGCTTGTTACTCAAAGTCAACCAAATCGGCTCAGTCACAGAGTCAATTGATGC
TCATTTGTTAGCAAAGAAAAATGGCTGGGGTACAATGGTCTCACATCGTTCAGGCGAAAC
TGAAGACTCATTCATTGCTGACTTGGTTGTTGGTCTTTCAACTGGTCAAATTAAGACTGG
TGCACCATGCCGTTCTGAGAGATTGTGCAAATACAATCAAATCTTACGTATTGAGGAAGA
ACTCGGAGCTAATGCCAAGTTTGCTGGCGAGAAATTCAGAAATCCACACGCCTAAATTTT
CAGCTGAATTTATTTCTCTTATTCTAATTTAATGCAACACAAAACATACAAAAATAAAGT
TAATTGATAAAAACATCCCATATGTCTATACTAGACCGAAATAATTCTGCATTTCCTCTT
GATTTCTCCTCCTTACATCGATGACTATCCATATCTTTATTAGAGATGCTCTGCTATGTA
TGCTATAAGTGAGGAGAAAGCAGACAGACATATGATTAATATCACATCACACAAGTATAA
CTACTATATTCATGATGTTAATCTCAAATTATCTAGTTAAGGATATTTTAAATATCTGTC
TCTCATTGAAAAAAGTTTCCTTAATAAAAGAAGACGTGAAAAGTTTTAAAGACACGCACT
CATAATAAAATGAAATGTAGGCTTCTTGATTTGTTGTTAATAAAGAAGAAAAATAAAAAA
GATGTTCAATGAATATAAAAATAGAATTTTGTTTTTAT
>g6296.t3 Gene=g6296 Length=393
MPIKSIKARQIFDSRGNPTVEVDLTTELGMFRAAVPSGASTGIHEALELRDKEAANWMGK
GVLKAVAHVNDSLGPEVIKSGLEVTQQREIDELMIKLDGTPNKSKFAFNVINGGEHAGNL
LAMQEFMLLPVGAKSFTEAMKMGAETYHNLAKVIQQKFGKDATAVGDEGGFAPNILDNRA
GLELIVEAIEKAGYTGKIKIGMDVAASEFKDEDGTYDLFKKDKVKDKSRNLSSDKLGDLY
MEFIKDFPVVSIEDPFDQDDWEAWTKLNARAGIQIVGDDLTVTNPKRIQTAVEKKACNCL
LLKVNQIGSVTESIDAHLLAKKNGWGTMVSHRSGETEDSFIADLVVGLSTGQIKTGAPCR
SERLCKYNQILRIEEELGANAKFAGEKFRNPHA
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g6296.t3 | CDD | cd03313 | enolase | 5 | 376 | 0.0 |
| 12 | g6296.t3 | Gene3D | G3DSA:3.20.20.120 | Enolase superfamily | 98 | 393 | 3.6E-133 |
| 5 | g6296.t3 | Hamap | MF_00318 | Enolase [eno]. | 1 | 377 | 35.8694 |
| 4 | g6296.t3 | PANTHER | PTHR11902 | ENOLASE | 1 | 106 | 3.6E-194 |
| 3 | g6296.t3 | PANTHER | PTHR11902 | ENOLASE | 107 | 390 | 3.6E-194 |
| 13 | g6296.t3 | PIRSF | PIRSF001400 | Enolase | 1 | 107 | 1.4E-38 |
| 14 | g6296.t3 | PIRSF | PIRSF001400 | Enolase | 106 | 390 | 3.2E-118 |
| 8 | g6296.t3 | PRINTS | PR00148 | Enolase signature | 35 | 49 | 8.8E-33 |
| 9 | g6296.t3 | PRINTS | PR00148 | Enolase signature | 277 | 288 | 8.8E-33 |
| 7 | g6296.t3 | PRINTS | PR00148 | Enolase signature | 300 | 314 | 8.8E-33 |
| 6 | g6296.t3 | PRINTS | PR00148 | Enolase signature | 329 | 346 | 8.8E-33 |
| 1 | g6296.t3 | Pfam | PF03952 | Enolase, N-terminal domain | 3 | 112 | 4.9E-41 |
| 2 | g6296.t3 | Pfam | PF00113 | Enolase, C-terminal TIM barrel domain | 107 | 390 | 3.3E-143 |
| 18 | g6296.t3 | ProSitePatterns | PS00164 | Enolase signature. | 300 | 313 | - |
| 19 | g6296.t3 | SFLD | SFLDG00178 | enolase | 107 | 377 | 0.0 |
| 20 | g6296.t3 | SFLD | SFLDS00001 | Enolase | 107 | 377 | 0.0 |
| 16 | g6296.t3 | SMART | SM01193 | Enolase_N_3 | 3 | 129 | 7.2E-58 |
| 17 | g6296.t3 | SMART | SM01192 | Enolase_C_3 | 107 | 391 | 1.9E-184 |
| 10 | g6296.t3 | SUPERFAMILY | SSF54826 | Enolase N-terminal domain-like | 3 | 113 | 1.83E-40 |
| 11 | g6296.t3 | SUPERFAMILY | SSF51604 | Enolase C-terminal domain-like | 108 | 390 | 1.77E-121 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0000287 | magnesium ion binding | MF |
| GO:0004634 | phosphopyruvate hydratase activity | MF |
| GO:0006096 | glycolytic process | BP |
| GO:0000015 | phosphopyruvate hydratase complex | CC |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
