General information for g63.t10
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 6A1.
Parent gene
- The parent gene g63 is located on chr_3: 447236..450989 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g63 | g63.t10 | TTS | g63.t10 | 446108 | 446108 |
| chr_3 | g63 | g63.t10 | isoform | g63.t10 | 446462 | 448301 |
| chr_3 | g63 | g63.t10 | exon | g63.t10.exon1 | 446462 | 446635 |
| chr_3 | g63 | g63.t10 | cds | g63.t10.CDS1 | 446462 | 446635 |
| chr_3 | g63 | g63.t10 | exon | g63.t10.exon2 | 446695 | 447107 |
| chr_3 | g63 | g63.t10 | cds | g63.t10.CDS2 | 446695 | 447107 |
| chr_3 | g63 | g63.t10 | exon | g63.t10.exon3 | 447169 | 447510 |
| chr_3 | g63 | g63.t10 | cds | g63.t10.CDS3 | 447169 | 447510 |
| chr_3 | g63 | g63.t10 | exon | g63.t10.exon4 | 447599 | 448301 |
| chr_3 | g63 | g63.t10 | cds | g63.t10.CDS4 | 447599 | 447779 |
| chr_3 | g63 | g63.t10 | TSS | g63.t10 | 448395 | 448395 |
Sequences
>g63.t10 Gene=g63 Length=1632
ATGGCGTCGTCATTAGTTCTCATAAGCATTAGTCTCTTATTTTTATATCTTTTTAATTTA
GTTAAAAAACAATACAATTATTTTAAAGATCGTGGAATACCAAACCCAAAGCCAATTTTC
CCATTAGGCAATTTTTGGGGTGTTGGATTATCTGTGCATTTTATTCATCGTGTAAATGAA
ATTTATAGACAATTTAAAGGCAAAGCAAAAATTTGTGGATTTTTTGTCACTACAATACCA
GCATATATCGCACTTGATATTGAATTGATCAAGAATATTCTAGTGAAAGATTTTGACTCA
TTTCATGATCGTGGAATCTATTATAATGAAAAAGCCGATCCCTTATCAGCGCATATCTTT
TCGGTAAATGACAAAAATTTAATAAGTAAAAAATCGCATGTGTCGATAACAGAAAATTTC
TTTTTATTTTTGTCTAATCTCAATCGCACCAACACATTGACTTCGCACTCAATATCAATG
TAATAGATAGCGGGTGAGCAATGGAAAACATTACGACAAAAAATGACTACGACATTTTCG
AGTGGCAAAATTAAAGCTATGATGCCGACCTTAAATAAAATTTCTGAAGAAATGACGGAA
GTACTTGGTGAATATGCAGATGAAGGCAAAACTTTGAATTATAGAGACATGGCATCACGT
CTTATGTGCGAAATTGTAGGAGAGATTGCATTTGGAATTAAATGTAATGCACTTCGTGAT
CCCAATTGTGAAATGATGGTATTAAGTGATTTTCTCAGTATTAAAGACATGAAACAAAGA
GTTATGTATTTATTTTCAAACGCATTTCCTGAAGTATGTCGTAAATTAAATATTCAAATC
ACACCCAAAGATGTACAACATTATTTTCTTAAAATTATCAACGAAGCAATTGAATATCGT
GAAAAGAACAAAATACGTAGAAATGATTATTTTGACATGCTTCTTCAGTTGAAAAATGAA
GGAAAACTTGATGGTGATGTGACAAATGTTGGAAGAATTTCACACACAGACATGTTGGCA
GCATGCTTTGTCTTTTTTGTAGCTGGCTTCGAAACTTCATCAACTGCACTCGCTTATGCT
CTCTATGAACTTGCAGTAAATCAAGAACTTCAAGATCGCACACGTGATGAAATTGAAAAA
GTTTTAAGTAATTATGATGGAAAAGTGACATATGAAGCTCTCATGAATATGAATTATCCT
GGTCAAGTTATTAGTGAATCACTTCGTAAATACACACCAGGAAATGTTTTGATGAGAAAA
TGTACAAAAGAATATAAAATTCCTGACACTGATATTGTAATTGAGAAAGGTCGAATGGTC
TTTTTACCAATGCATGCCATTCATAATGATCCTGAGTACTTCCCTGATCCTGAAAAATTT
GATCCTGATAGATTTTCTCCTGAAATGGAAAAAGAACGAAATCCATTTACATTTTTACCA
TTTGGTGAAGGAATGAGAATCTGCATGGGTCTACGTTTCGGTCTAATTCAAACACGTCTT
GGCCTTGTTTCGGTTTTACGTAAATACCGTGTAACACTCAATGAGAAAACAGTTCATCCT
ATTCGTCTTGATCCAAAAGATGTCACAATGATGCCTTACGGTGGTATTTGGCTCAACACA
GAACACATCTGA
>g63.t10 Gene=g63 Length=369
MTTTFSSGKIKAMMPTLNKISEEMTEVLGEYADEGKTLNYRDMASRLMCEIVGEIAFGIK
CNALRDPNCEMMVLSDFLSIKDMKQRVMYLFSNAFPEVCRKLNIQITPKDVQHYFLKIIN
EAIEYREKNKIRRNDYFDMLLQLKNEGKLDGDVTNVGRISHTDMLAACFVFFVAGFETSS
TALAYALYELAVNQELQDRTRDEIEKVLSNYDGKVTYEALMNMNYPGQVISESLRKYTPG
NVLMRKCTKEYKIPDTDIVIEKGRMVFLPMHAIHNDPEYFPDPEKFDPDRFSPEMEKERN
PFTFLPFGEGMRICMGLRFGLIQTRLGLVSVLRKYRVTLNEKTVHPIRLDPKDVTMMPYG
GIWLNTEHI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 15 | g63.t10 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 1 | 369 | 6.5E-99 |
| 2 | g63.t10 | PANTHER | PTHR24292 | CYTOCHROME P450 | 1 | 368 | 3.5E-128 |
| 3 | g63.t10 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 1 | 368 | 3.5E-128 |
| 7 | g63.t10 | PRINTS | PR00463 | E-class P450 group I signature | 163 | 180 | 4.0E-19 |
| 12 | g63.t10 | PRINTS | PR00385 | P450 superfamily signature | 174 | 191 | 3.8E-10 |
| 8 | g63.t10 | PRINTS | PR00463 | E-class P450 group I signature | 183 | 209 | 4.0E-19 |
| 10 | g63.t10 | PRINTS | PR00385 | P450 superfamily signature | 228 | 239 | 3.8E-10 |
| 4 | g63.t10 | PRINTS | PR00463 | E-class P450 group I signature | 269 | 293 | 4.0E-19 |
| 6 | g63.t10 | PRINTS | PR00463 | E-class P450 group I signature | 304 | 314 | 4.0E-19 |
| 11 | g63.t10 | PRINTS | PR00385 | P450 superfamily signature | 305 | 314 | 3.8E-10 |
| 5 | g63.t10 | PRINTS | PR00463 | E-class P450 group I signature | 314 | 337 | 4.0E-19 |
| 9 | g63.t10 | PRINTS | PR00385 | P450 superfamily signature | 314 | 325 | 3.8E-10 |
| 1 | g63.t10 | Pfam | PF00067 | Cytochrome P450 | 1 | 362 | 5.8E-78 |
| 14 | g63.t10 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 307 | 316 | - |
| 13 | g63.t10 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 2 | 365 | 7.07E-86 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed