General information for g63.t16
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Cytochrome P450 6a22.
Parent gene
- The parent gene g63 is located on chr_3: 447236..450989 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g63 | g63.t16 | isoform | g63.t16 | 447236 | 448301 |
| chr_3 | g63 | g63.t16 | exon | g63.t16.exon1 | 447236 | 447815 |
| chr_3 | g63 | g63.t16 | cds | g63.t16.CDS1 | 447573 | 447815 |
| chr_3 | g63 | g63.t16 | exon | g63.t16.exon2 | 447939 | 448301 |
| chr_3 | g63 | g63.t16 | cds | g63.t16.CDS2 | 447939 | 448301 |
| chr_3 | g63 | g63.t16 | TSS | g63.t16 | 448395 | 448395 |
| chr_3 | g63 | g63.t16 | TTS | g63.t16 | NA | NA |
Sequences
>g63.t16 Gene=g63 Length=943
ATGGCGTCGTCATTAGTTCTCATAAGCATTAGTCTCTTATTTTTATATCTTTTTAATTTA
GTTAAAAAACAATACAATTATTTTAAAGATCGTGGAATACCAAACCCAAAGCCAATTTTC
CCATTAGGCAATTTTTGGGGTGTTGGATTATCTGTGCATTTTATTCATCGTGTAAATGAA
ATTTATAGACAATTTAAAGGCAAAGCAAAAATTTGTGGATTTTTTGTCACTACAATACCA
GCATATATCGCACTTGATATTGAATTGATCAAGAATATTCTAGTGAAAGATTTTGACTCA
TTTCATGATCGTGGAATCTATTATAATGAAAAAGCCGATCCCTTATCAGCGCATATCTTT
TCGATAGCGGGTGAGCAATGGAAAACATTACGACAAAAAATGACTACGACATTTTCGAGT
GGCAAAATTAAAGCTATGATGCCGACCTTAAATAAAATTTCTGAAGAAATGACGGAAGTA
CTTGGTGAATATGCAGATGAAGGCAAAACTTTGAATTATAGAGACATGGCATCACGTCTT
ATGTGCGAAATTGTAGGAGAGATTGCATTTGGAATTAAATGTAAGAAAACCAGCAAACAA
AATTAACTGAGTTGTTAAAATTGAAAGCCAACTGACAACAAAGTTTCTCTAATTTAAAAT
TATTTTAGGTAATGCACTTCGTGATCCCAATTGTGAAATGATGGTATTAAGTGATTTTCT
CAGTATTAAAGACATGAAACAAAGAGTTATGTATTTATTTTCAAACGCATTTCCTGAAGT
ATGTCGTAAATTAAATATTCAAATCACACCCAAAGATGTACAACATTATTTTCTTAAAAT
TATCAACGAAGCAATTGAATATCGTGAAAAGAACAAAATACGTAGAAATGATTATTTTGA
CATGCTTCTTCAGTTGAAAAATGAAGGAAAACTTGATGGTGAT
>g63.t16 Gene=g63 Length=201
MASSLVLISISLLFLYLFNLVKKQYNYFKDRGIPNPKPIFPLGNFWGVGLSVHFIHRVNE
IYRQFKGKAKICGFFVTTIPAYIALDIELIKNILVKDFDSFHDRGIYYNEKADPLSAHIF
SIAGEQWKTLRQKMTTTFSSGKIKAMMPTLNKISEEMTEVLGEYADEGKTLNYRDMASRL
MCEIVGEIAFGIKCKKTSKQN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 8 | g63.t16 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 16 | 201 | 1.8E-36 |
| 2 | g63.t16 | PANTHER | PTHR24292 | CYTOCHROME P450 | 9 | 199 | 3.1E-54 |
| 3 | g63.t16 | PANTHER | PTHR24292:SF95 | CYP6A16, ISOFORM B-RELATED | 9 | 199 | 3.1E-54 |
| 1 | g63.t16 | Pfam | PF00067 | Cytochrome P450 | 44 | 195 | 4.3E-19 |
| 10 | g63.t16 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 23 | - |
| 11 | g63.t16 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 4 | - |
| 12 | g63.t16 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 5 | 18 | - |
| 13 | g63.t16 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 19 | 23 | - |
| 9 | g63.t16 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 24 | 201 | - |
| 6 | g63.t16 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 33 | 195 | 3.41E-27 |
| 7 | g63.t16 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 23 | - |
| 5 | g63.t16 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 4 | 21 | - |
| 4 | g63.t16 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 71 | 90 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed