General information for g6301.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Venom carboxylesterase-6.
Parent gene
- The parent gene g6301 is located on chr_2: 16039120..16041101 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6301 | g6301.t1 | TSS | g6301.t1 | 16038768 | 16038768 |
| chr_2 | g6301 | g6301.t1 | isoform | g6301.t1 | 16039120 | 16041101 |
| chr_2 | g6301 | g6301.t1 | exon | g6301.t1.exon1 | 16039120 | 16039734 |
| chr_2 | g6301 | g6301.t1 | cds | g6301.t1.CDS1 | 16039120 | 16039734 |
| chr_2 | g6301 | g6301.t1 | exon | g6301.t1.exon2 | 16039787 | 16040035 |
| chr_2 | g6301 | g6301.t1 | cds | g6301.t1.CDS2 | 16039787 | 16040035 |
| chr_2 | g6301 | g6301.t1 | exon | g6301.t1.exon3 | 16040089 | 16040430 |
| chr_2 | g6301 | g6301.t1 | cds | g6301.t1.CDS3 | 16040089 | 16040430 |
| chr_2 | g6301 | g6301.t1 | exon | g6301.t1.exon4 | 16040489 | 16040778 |
| chr_2 | g6301 | g6301.t1 | cds | g6301.t1.CDS4 | 16040489 | 16040778 |
| chr_2 | g6301 | g6301.t1 | exon | g6301.t1.exon5 | 16040891 | 16041101 |
| chr_2 | g6301 | g6301.t1 | cds | g6301.t1.CDS5 | 16040891 | 16041101 |
| chr_2 | g6301 | g6301.t1 | TTS | g6301.t1 | NA | NA |
Sequences
>g6301.t1 Gene=g6301 Length=1707
ATGAAAGAAATATTAATTTGTGTATTTTATTTTACATCTTTAACATTTGCAAGAACTTCA
CCAGAATTAGAAGTGAATTTAACAGAAGGGAAAATCCTTGGTCGTTTTAAGACTTCAAAT
TCAGGCAGAACAATTCGAGCATTTATGGGAATTCCATTTGCTGAGCCTCCAATCAATGAT
TTACGATTTCGAGCGCCATTCAAGAAAAAGCCTTGGCGACCAAATACTTTAGTGACTCAA
AAAGAACCAAATAAATGTCCACAAGTAAATCAACATGTTTTTGAAGGAAATGAAGATTGT
CTTTATTTAAATGTTTATGCACCAGAAGTTAAATTTAAAGAAAAATATCCAGTAATTGTA
TTTTTCCATGGTGGAAGTTTTTTAACAGGATCTGGAGGAATTTCTGAGCATGGACCTGAT
TACTTTTTAGACACCGATGTGATTTTAGTTGTTGGAAATTATCGACTCGGTGCACTTGGA
TTTTTATCAACTGAAGATGAATATTCACCTGGAAATTATGGTTTAAAAGATCAAGTTGCA
CTATTGAGATGGGTTAATGAAAATATTGAAAGTTTTGGAGGAGATATAAATTTAATTACA
ATTTTTGGTGAATCTGTTGGAGGTTCATCTGTAAATTTTCATATGATCTCACCGCTGTCG
CAAAATCTTTTTCATCGAGGAATTTCACAATCTGGAACTTTACAAAGCTTTTGGGCTGAT
CCATTACAAAATGGAATTGCTAAAGAAAGAGCTGAAAAAGTAGCAAAAAGTTTAAATTGC
TCAGATATTGAAAATTCTGAAGTTATCATGAATTGTCTAAGAAATCAATCAACTGAAAGT
TTAACAAATAGTATTTTTACATTTGGAGATATGTTAGAATATACTTCACCATTTTTACCA
GTCATGGAAAATCTCACGCAAACAATTGAAGAACCATTCATTACAGTAGAGACCTTCAAG
TACAATAACATTGCAAAGAAAATTCCATGGTTAGTTGGAATTAATAGCGAAGAAGGTGCT
TTAGTTTCATCATTTTTATATAGCGATGATGGAGTGAAATTATTTGAATGGACAGATAAA
ATACCAAAATATTTAGGATACGATCATTTAGATGAATTTGATCAAGAAGAAATAACAAAG
GAAATTCGAGAATTTTATTTTAATAATGAAGCAATAACGTATGCAAAATTTGAGAATATT
ACAAATCTTTTAACTGATGGTGGTGGATATTTCGGAATGTTTGACGTCCTTGAAGCTCGA
TTAAAAAACAATCATTTAGACAATACTTTTGTTTATTTTTATTCACATAAAGGTGGTGCA
AGTTTCACAGAAGTAACTGGTCGACAAAAATTTCTTGGAACTTCGCATATGGATGAACTT
TTGAATCTATTTCCACTTTATGAATCTCAACAATTTTATTCATCTGCTCCAACTGATTTT
GACAGAGCGTTGCAAAAAATTATGCCAAATTTATGGGTTAATTTCGCGAGAACTGGAAAT
CCAAATTTAGGTCATGTAAATCCATTTACACATTCAGATCAATTTTGGAAATCAGCTAAA
AATTTTCCACTTGATTATATGAGAATAGGCAATGAAAATGGAAAATCTAGAAGATTGTTG
GAAATGAAGAATGGCTTACTCACTGAAAGAGTTGAATTTTGGAGGAGATTGAAATCAAAT
TATCCAATAATTCAATGGAAACTTTAA
>g6301.t1 Gene=g6301 Length=568
MKEILICVFYFTSLTFARTSPELEVNLTEGKILGRFKTSNSGRTIRAFMGIPFAEPPIND
LRFRAPFKKKPWRPNTLVTQKEPNKCPQVNQHVFEGNEDCLYLNVYAPEVKFKEKYPVIV
FFHGGSFLTGSGGISEHGPDYFLDTDVILVVGNYRLGALGFLSTEDEYSPGNYGLKDQVA
LLRWVNENIESFGGDINLITIFGESVGGSSVNFHMISPLSQNLFHRGISQSGTLQSFWAD
PLQNGIAKERAEKVAKSLNCSDIENSEVIMNCLRNQSTESLTNSIFTFGDMLEYTSPFLP
VMENLTQTIEEPFITVETFKYNNIAKKIPWLVGINSEEGALVSSFLYSDDGVKLFEWTDK
IPKYLGYDHLDEFDQEEITKEIREFYFNNEAITYAKFENITNLLTDGGGYFGMFDVLEAR
LKNNHLDNTFVYFYSHKGGASFTEVTGRQKFLGTSHMDELLNLFPLYESQQFYSSAPTDF
DRALQKIMPNLWVNFARTGNPNLGHVNPFTHSDQFWKSAKNFPLDYMRIGNENGKSRRLL
EMKNGLLTERVEFWRRLKSNYPIIQWKL
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g6301.t1 | Gene3D | G3DSA:3.40.50.1820 | - | 2 | 562 | 5.6E-138 |
| 2 | g6301.t1 | PANTHER | PTHR11559:SF395 | CARBOXYLIC ESTER HYDROLASE | 3 | 560 | 6.3E-140 |
| 3 | g6301.t1 | PANTHER | PTHR11559 | CARBOXYLESTERASE | 3 | 560 | 6.3E-140 |
| 1 | g6301.t1 | Pfam | PF00135 | Carboxylesterase family | 23 | 554 | 4.6E-120 |
| 9 | g6301.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 17 | - |
| 10 | g6301.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 11 | g6301.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 12 | - |
| 12 | g6301.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 17 | - |
| 8 | g6301.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 18 | 568 | - |
| 5 | g6301.t1 | ProSitePatterns | PS00941 | Carboxylesterases type-B signature 2. | 98 | 108 | - |
| 4 | g6301.t1 | SUPERFAMILY | SSF53474 | alpha/beta-Hydrolases | 23 | 556 | 1.68E-111 |
| 6 | g6301.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 17 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
