General information for g6320.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Pickpocket protein 28.
Parent gene
- The parent gene g6320 is located on chr_2: 16185027..16187234 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6320 | g6320.t1 | isoform | g6320.t1 | 16185027 | 16187234 |
| chr_2 | g6320 | g6320.t1 | exon | g6320.t1.exon1 | 16185027 | 16185141 |
| chr_2 | g6320 | g6320.t1 | cds | g6320.t1.CDS1 | 16185027 | 16185141 |
| chr_2 | g6320 | g6320.t1 | exon | g6320.t1.exon2 | 16185197 | 16185362 |
| chr_2 | g6320 | g6320.t1 | cds | g6320.t1.CDS2 | 16185197 | 16185362 |
| chr_2 | g6320 | g6320.t1 | exon | g6320.t1.exon3 | 16185422 | 16185507 |
| chr_2 | g6320 | g6320.t1 | cds | g6320.t1.CDS3 | 16185422 | 16185507 |
| chr_2 | g6320 | g6320.t1 | exon | g6320.t1.exon4 | 16185815 | 16186496 |
| chr_2 | g6320 | g6320.t1 | cds | g6320.t1.CDS4 | 16185815 | 16186496 |
| chr_2 | g6320 | g6320.t1 | exon | g6320.t1.exon5 | 16186553 | 16187234 |
| chr_2 | g6320 | g6320.t1 | cds | g6320.t1.CDS5 | 16186553 | 16187234 |
| chr_2 | g6320 | g6320.t1 | TSS | g6320.t1 | NA | NA |
| chr_2 | g6320 | g6320.t1 | TTS | g6320.t1 | NA | NA |
Sequences
>g6320.t1 Gene=g6320 Length=1731
ATGAAACGAAGCATCTCAAAACAGCTGTTAAATACTTATGCAAGTGAATCAACAATTCAC
GGTGTTCGTTATATAGTCGGTGGTGATAATCAAAATAGATCAATAAGAATCTTTTGGATT
TGTGCATTTTTCTTATCAATTATTGGTTTGGGTTATTATGCTTATGGTGTCTATATGAAA
TGGAGCATAAATCCAGACATTGGCCTTACAATTCGAATGAGACCAATGAGAGAAATTCCA
TTTCCAGCCATCACAATTTGTTCACCATTATTTGCTCGTGATAGTCTTGCAAATTATTCA
AATTATTATGCAGAATTTACATCAAAAAGTGGCAAAATTATTCCATTTTTAAAACCAGAA
GAACAAAATTATCTTGCAGCAAATATTCAAGCATGTTCACCAACATCATCATCAATGGTT
ATGAAAGGCTGTGCAAACCGTAGTGATAAGAATTTTCCAAAATTATTGCGTGAATCATCA
CTTGATGTTTCAGAATTATTTGCGGCATGTCGATTTAAAAAGAATTACATGAAATGTGAA
AAAATTTTAAATCGTGTTTTAACAAACAACGGATATTGTTATACATTAAACATGCAAGGT
TATCATACAATTTTTAATAATGAAATTAGCAAACATTTTGAGTCTTATCGAAGGAAGACA
ATTAGTAAATCATTTGATAAAGAACAAAAACTCTACAACGAAACTTTCAATGATGAAAAA
GACAAAGAAATTTGGACACTTGACAAAGGTTACACAACAAATGATGACGATGTTTTTCCA
ATTCGAGCCATTAAAATTAATCAACTTAGTGTCTACATGAAACTAACTGAAAAAGATGCA
ACAAATCTTTGTCTATCACAAGGCAAAGGTTATAAAATAATTTTTCATCTTCCAAATGAA
ATTCCAACACCTTTCCATGATGAATATTTTATTCCGTTCAATTTTGAACGAATGATGACT
TTAACAGCAAAATCAATTCGTCCTGATCCTGAGCTTAAAAAATATTCTCCATCTGCACGA
AGATGTTACTTTGAAGGAGAAAGAAAATTAAAATTTTTCAATTCTTATACGAAAGCTCAT
TGTGATTATGAATGCATGACAAACTACACTTTGAAAGTTTGCGGATGTGTTAAATTCTCA
ATGCCAAGAGATGATAAAACTCAAGTTTGTGATTTAAATAAAGCAAAATGCTACTTTAAT
GCTATGATGAGTTGGCCTGATAAAGAAGAAATTGAAAAAGATGGAATTACACCTTGCAAT
TGTTTACCAACTTGCAGTGATGTTAAATATAGCATTAAGTTGGATAAAGAAGCTGAATTG
GAAGCGAATATTCGATTGGCTCATGTTAAGAATTTAGATGAAACTAATATTTACTCTCGA
ATGGCAATAAGATTCAACGAGCATTCAATTGAAGAACAAGAAAATTTTGTCGCATATAAA
TTACAAAATTTCATAGCAGATTTTGGTGGGTTATTAGGTCTTTTTATGGGCTGCTCATTA
TTATCAATTGTAGAATTAATTTTTCATTGCATTCGAGCATGCATCAATAAACGAGGAAAA
AATGAAATGAAATTAGACCAAATAGAAGAAAATATTCAAACAGAAAACAGAATTCATAAT
CAATTGAACAATAAGGAATTTTATCATCCACATAATAACAATGATCCATGTTGTAATGTA
GTGCGTAATAATGATGCTGTGACAGTTGTTACACTGAACAAACTAAATTAA
>g6320.t1 Gene=g6320 Length=576
MKRSISKQLLNTYASESTIHGVRYIVGGDNQNRSIRIFWICAFFLSIIGLGYYAYGVYMK
WSINPDIGLTIRMRPMREIPFPAITICSPLFARDSLANYSNYYAEFTSKSGKIIPFLKPE
EQNYLAANIQACSPTSSSMVMKGCANRSDKNFPKLLRESSLDVSELFAACRFKKNYMKCE
KILNRVLTNNGYCYTLNMQGYHTIFNNEISKHFESYRRKTISKSFDKEQKLYNETFNDEK
DKEIWTLDKGYTTNDDDVFPIRAIKINQLSVYMKLTEKDATNLCLSQGKGYKIIFHLPNE
IPTPFHDEYFIPFNFERMMTLTAKSIRPDPELKKYSPSARRCYFEGERKLKFFNSYTKAH
CDYECMTNYTLKVCGCVKFSMPRDDKTQVCDLNKAKCYFNAMMSWPDKEEIEKDGITPCN
CLPTCSDVKYSIKLDKEAELEANIRLAHVKNLDETNIYSRMAIRFNEHSIEEQENFVAYK
LQNFIADFGGLLGLFMGCSLLSIVELIFHCIRACINKRGKNEMKLDQIEENIQTENRIHN
QLNNKEFYHPHNNNDPCCNVVRNNDAVTVVTLNKLN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 11 | g6320.t1 | Coils | Coil | Coil | 525 | 545 | - |
| 2 | g6320.t1 | PANTHER | PTHR11690 | AMILORIDE-SENSITIVE SODIUM CHANNEL-RELATED | 8 | 542 | 6.6E-55 |
| 3 | g6320.t1 | PANTHER | PTHR11690:SF243 | PICKPOCKET 12-RELATED | 8 | 542 | 6.6E-55 |
| 4 | g6320.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 77 | 93 | 7.4E-9 |
| 8 | g6320.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 290 | 308 | 7.4E-9 |
| 6 | g6320.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 367 | 384 | 7.4E-9 |
| 7 | g6320.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 475 | 489 | 7.4E-9 |
| 5 | g6320.t1 | PRINTS | PR01078 | Amiloride-sensitive sodium channel alpha-subunit signature | 489 | 505 | 7.4E-9 |
| 1 | g6320.t1 | Pfam | PF00858 | Amiloride-sensitive sodium channel | 13 | 508 | 2.4E-76 |
| 13 | g6320.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 36 | - |
| 16 | g6320.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 37 | 55 | - |
| 14 | g6320.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 56 | 487 | - |
| 15 | g6320.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 488 | 515 | - |
| 12 | g6320.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 516 | 576 | - |
| 9 | g6320.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 37 | 59 | - |
| 10 | g6320.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 489 | 511 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016020 | membrane | CC |
| GO:0005272 | sodium channel activity | MF |
| GO:0006814 | sodium ion transport | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed