General information for g635.t5
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial.
Parent gene
- The parent gene g635 is located on chr_3: 4682651..4683238 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g635 | g635.t5 | TSS | g635.t5 | 4679520 | 4679520 |
| chr_3 | g635 | g635.t5 | isoform | g635.t5 | 4679597 | 4680601 |
| chr_3 | g635 | g635.t5 | exon | g635.t5.exon1 | 4679597 | 4680068 |
| chr_3 | g635 | g635.t5 | exon | g635.t5.exon2 | 4680127 | 4680255 |
| chr_3 | g635 | g635.t5 | cds | g635.t5.CDS1 | 4680149 | 4680255 |
| chr_3 | g635 | g635.t5 | exon | g635.t5.exon3 | 4680321 | 4680601 |
| chr_3 | g635 | g635.t5 | cds | g635.t5.CDS2 | 4680321 | 4680600 |
| chr_3 | g635 | g635.t5 | TTS | g635.t5 | NA | NA |
Sequences
>g635.t5 Gene=g635 Length=882
ATGATTCGATCACTTTCCTCATTTCCACGAAGTGTTGTTCTTCGCATAAGCAATAGAAGT
AATCAGGTACAACATCAGTTTCAGTGTAAAAAGTGTTGAAATAGTTTTTAGATTGCGTAA
TTTTTCTTCCTTGAATTCTCGAGCCGAAAAAAAGTGTGAACAAAAGAAAAAATTTAAAAA
AGTATCTCAAATTACTTTCTATTTCATTTAAAAATGGCCTTCAATGTGCTTGTTTTGATG
ATTACAATTAAATCTGCACGTGGTCATGTGTTCAACATTTTGATTAGATCGTGATGACTC
ACTTTATTTTATTTTTATTTAGTCTGCGTGTTTTCATTCTCTCTTTCTCTAATTCTTTTT
TATATTTATAAAAAACGTGATTAAAGATCCAGCAAACGTTGAGGAATAATATTCATCAAC
AATGTGCCGTTCAAAAGTTGGTGCTTACATGCGACACAAATTCTCAAAATTCAACACTTA
AAAATAGATTAGTTATGCAGCAACAACATGTTAGATTAATTCAAACAACTACGCAACTTC
TTAATTCTGAAATCGTTAAGGTGCCACCATTTGCCGATTCTGTATCAGAAGGTGATGTAA
AATTTGATAAGAAAGTAGGTGATGCAGTTGCAGCTGATGAGGTTGTTATGGAAATTGAAA
CCGATAAGACAACAGTTGGTGTTCCAGCTCCATCGCATGGTATCATTGAGGAAATTTTTG
TTTCAGACGGTGATACAGTAAAGGCTGGACAACAGTTGTTCAAAATGAAGTTTACAGATG
CCGCACCAGAAAAAGAAGCATCAACTTCCACGCCAGAACCTCCAAAAGCAGCTGCTGCAC
CTTCACCACCACCACCACAACCTAAACAAGAAGCACCAAAAG
>g635.t5 Gene=g635 Length=129
MQQQHVRLIQTTTQLLNSEIVKVPPFADSVSEGDVKFDKKVGDAVAADEVVMEIETDKTT
VGVPAPSHGIIEEIFVSDGDTVKAGQQLFKMKFTDAAPEKEASTSTPEPPKAAAAPSPPP
PQPKQEAPK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g635.t5 | CDD | cd06849 | lipoyl_domain | 20 | 91 | 3.49813E-20 |
| 5 | g635.t5 | Gene3D | G3DSA:2.40.50.100 | - | 16 | 118 | 3.7E-19 |
| 9 | g635.t5 | MobiDBLite | mobidb-lite | consensus disorder prediction | 92 | 129 | - |
| 8 | g635.t5 | MobiDBLite | mobidb-lite | consensus disorder prediction | 111 | 129 | - |
| 2 | g635.t5 | PANTHER | PTHR43416 | DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL-RELATED | 6 | 126 | 1.7E-24 |
| 3 | g635.t5 | PANTHER | PTHR43416:SF18 | DIHYDROLIPOYLLYSINE-RESIDUE SUCCINYLTRANSFERASE COMPONENT OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX, MITOCHONDRIAL | 6 | 126 | 1.7E-24 |
| 1 | g635.t5 | Pfam | PF00364 | Biotin-requiring enzyme | 21 | 91 | 9.7E-19 |
| 7 | g635.t5 | ProSitePatterns | PS00189 | 2-oxo acid dehydrogenases acyltransferase component lipoyl binding site. | 42 | 71 | - |
| 10 | g635.t5 | ProSiteProfiles | PS50968 | Biotinyl/lipoyl domain profile. | 18 | 92 | 24.11 |
| 4 | g635.t5 | SUPERFAMILY | SSF51230 | Single hybrid motif | 19 | 110 | 8.25E-21 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
There are no GO annotations for this transcript.
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
