General information for g6355.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Guanine nucleotide-binding protein subunit beta-2.
Parent gene
- The parent gene g6355 is located on chr_2: 16449350..16451102 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6355 | g6355.t1 | isoform | g6355.t1 | 16449350 | 16451102 |
| chr_2 | g6355 | g6355.t1 | exon | g6355.t1.exon1 | 16449350 | 16449543 |
| chr_2 | g6355 | g6355.t1 | cds | g6355.t1.CDS1 | 16449350 | 16449543 |
| chr_2 | g6355 | g6355.t1 | exon | g6355.t1.exon2 | 16449620 | 16449671 |
| chr_2 | g6355 | g6355.t1 | cds | g6355.t1.CDS2 | 16449620 | 16449671 |
| chr_2 | g6355 | g6355.t1 | exon | g6355.t1.exon3 | 16449733 | 16449817 |
| chr_2 | g6355 | g6355.t1 | cds | g6355.t1.CDS3 | 16449733 | 16449817 |
| chr_2 | g6355 | g6355.t1 | exon | g6355.t1.exon4 | 16450267 | 16450764 |
| chr_2 | g6355 | g6355.t1 | cds | g6355.t1.CDS4 | 16450267 | 16450764 |
| chr_2 | g6355 | g6355.t1 | exon | g6355.t1.exon5 | 16450888 | 16451102 |
| chr_2 | g6355 | g6355.t1 | cds | g6355.t1.CDS5 | 16450888 | 16451102 |
| chr_2 | g6355 | g6355.t1 | TSS | g6355.t1 | NA | NA |
| chr_2 | g6355 | g6355.t1 | TTS | g6355.t1 | NA | NA |
Sequences
>g6355.t1 Gene=g6355 Length=1044
ATGCCAAAAGATGACGCAGAAACGGCCGCTTTGAAGAATGAGCTTAATGAGCTTATCGCA
AAATGCAAAGAGGAAGCAAAGAAACAAGCTGACTGCAAATTGGAAGACAAATGTGGAGAT
TTAGGCGCGGTTCCAAAATGTCAACTTTCAACCAAGAAAATGCTTAAAGGACATATCAAT
AAAGTCAATTCTGTTCATTATTCTGGTGATTCTAGATATTGCGTATCCGGTTCACTCGAT
GGAAAATTAATTGTTTGGGATACTTGGACGGGTAACAAAGTGCAAGTTATTCCACTTAGA
TCATCATGGGTAATGTCAGTCGCATATTCAACATCAGGAAATTTTGTAGCATGTGGTGGA
ATGGACAATATGTGCACTATCTATGATGTCAATAATCGTGATGCATCGGGAAATGCAAAA
ATTGTACGTGAATTGGCAGGTTATGAAGGATTCTTGAGTTCATGTCGTTTTCTCGATGAT
CGACACATTCTCACTGGCTCTGGTGATATGAAACTTTGTGTTTGGGACCTTGAATCGGGA
AAAAAGACAACCGAAATTGAAGCTGCACACGCAGGCGATGTTGTATCGCTTTCACTTTCC
CCTGATGGCAATCAATTTGTCACGGGTTCAGTCGATCGAACTTGCAAGCTTTGGGATGTA
CGTGATTGCAAACAGAAGCAAACATTCTTTGGTCATTCATCAGACGTTAACTGTGTAAGT
TTTCATCCAAGTGGCTACGCATTTGCTACAGGATCTGAAGATAAAACTGCAAGACTCTTT
GATATTCGTTCAGATCAGCAAATTGGTCATTATGAGCCACCAAACAAAACATCTGGATTT
ACTTCATGCGCTCTTTCGCTAAGTGGTCGACTGCTCTTGTGCGGATCTGACGATAATAAC
ATTCACGTTTGGGATACACTCAAAATACAACATAATGGCACGCTCGGTGGTCATGAGAAT
CGCGTAACTTCCATCTGCATGGCACCAAACGGTATGGCACTCGTTTCATCGAGTTGGGAT
CAATTTGTTCGTGTTTGGGTTTAA
>g6355.t1 Gene=g6355 Length=347
MPKDDAETAALKNELNELIAKCKEEAKKQADCKLEDKCGDLGAVPKCQLSTKKMLKGHIN
KVNSVHYSGDSRYCVSGSLDGKLIVWDTWTGNKVQVIPLRSSWVMSVAYSTSGNFVACGG
MDNMCTIYDVNNRDASGNAKIVRELAGYEGFLSSCRFLDDRHILTGSGDMKLCVWDLESG
KKTTEIEAAHAGDVVSLSLSPDGNQFVTGSVDRTCKLWDVRDCKQKQTFFGHSSDVNCVS
FHPSGYAFATGSEDKTARLFDIRSDQQIGHYEPPNKTSGFTSCALSLSGRLLLCGSDDNN
IHVWDTLKIQHNGTLGGHENRVTSICMAPNGMALVSSSWDQFVRVWV
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 20 | g6355.t1 | CDD | cd00200 | WD40 | 52 | 346 | 3.44162E-84 |
| 18 | g6355.t1 | Coils | Coil | Coil | 8 | 28 | - |
| 17 | g6355.t1 | Gene3D | G3DSA:2.130.10.10 | - | 6 | 346 | 1.1E-112 |
| 7 | g6355.t1 | PANTHER | PTHR19850:SF30 | GUANINE NUCLEOTIDE-BINDING PROTEIN SUBUNIT BETA-2 | 2 | 347 | 4.9E-199 |
| 8 | g6355.t1 | PANTHER | PTHR19850 | GUANINE NUCLEOTIDE-BINDING PROTEIN BETA G PROTEIN BETA | 2 | 347 | 4.9E-199 |
| 19 | g6355.t1 | PIRSF | PIRSF002394 | GNBP_B | 1 | 347 | 1.2E-137 |
| 14 | g6355.t1 | PRINTS | PR00319 | Beta G protein (transducin) signature | 55 | 71 | 2.7E-21 |
| 12 | g6355.t1 | PRINTS | PR00319 | Beta G protein (transducin) signature | 74 | 88 | 2.7E-21 |
| 13 | g6355.t1 | PRINTS | PR00319 | Beta G protein (transducin) signature | 93 | 108 | 2.7E-21 |
| 15 | g6355.t1 | PRINTS | PR00319 | Beta G protein (transducin) signature | 111 | 128 | 2.7E-21 |
| 11 | g6355.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 163 | 177 | 6.2E-7 |
| 10 | g6355.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 206 | 220 | 6.2E-7 |
| 9 | g6355.t1 | PRINTS | PR00320 | G protein beta WD-40 repeat signature | 292 | 306 | 6.2E-7 |
| 2 | g6355.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 53 | 87 | 7.4E-6 |
| 1 | g6355.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 102 | 129 | 0.022 |
| 6 | g6355.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 189 | 219 | 1.3E-6 |
| 4 | g6355.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 226 | 261 | 9.1E-7 |
| 3 | g6355.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 280 | 305 | 0.0022 |
| 5 | g6355.t1 | Pfam | PF00400 | WD domain, G-beta repeat | 311 | 346 | 1.4E-5 |
| 28 | g6355.t1 | ProSitePatterns | PS00678 | Trp-Asp (WD) repeats signature. | 74 | 88 | - |
| 29 | g6355.t1 | ProSitePatterns | PS00678 | Trp-Asp (WD) repeats signature. | 163 | 177 | - |
| 30 | g6355.t1 | ProSiteProfiles | PS50294 | Trp-Asp (WD) repeats circular profile. | 55 | 347 | 57.792 |
| 31 | g6355.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 55 | 96 | 13.984 |
| 33 | g6355.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 145 | 185 | 9.74 |
| 36 | g6355.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 187 | 228 | 14.786 |
| 34 | g6355.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 229 | 270 | 13.85 |
| 35 | g6355.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 273 | 305 | 8.537 |
| 32 | g6355.t1 | ProSiteProfiles | PS50082 | Trp-Asp (WD) repeats profile. | 315 | 347 | 13.984 |
| 23 | g6355.t1 | SMART | SM00320 | WD40_4 | 48 | 87 | 2.2E-7 |
| 24 | g6355.t1 | SMART | SM00320 | WD40_4 | 90 | 129 | 1.6E-4 |
| 22 | g6355.t1 | SMART | SM00320 | WD40_4 | 138 | 176 | 8.3E-5 |
| 25 | g6355.t1 | SMART | SM00320 | WD40_4 | 179 | 219 | 1.5E-8 |
| 21 | g6355.t1 | SMART | SM00320 | WD40_4 | 222 | 261 | 5.5E-10 |
| 26 | g6355.t1 | SMART | SM00320 | WD40_4 | 264 | 305 | 0.0011 |
| 27 | g6355.t1 | SMART | SM00320 | WD40_4 | 308 | 347 | 6.1E-5 |
| 16 | g6355.t1 | SUPERFAMILY | SSF50978 | WD40 repeat-like | 21 | 346 | 2.12E-81 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0007165 | signal transduction | BP |
| GO:0005515 | protein binding | MF |
KEGG
Orthology
- This transcript has been annotated with K07972 (guanine nucleotide-binding protein subunit beta, other)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed