Gene loci information

Transcript annotation

  • This transcript has been annotated as Putative Mitochondrial fission 1 protein.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_3 g642 g642.t2 TTS g642.t2 4863833 4863833
chr_3 g642 g642.t2 isoform g642.t2 4864620 4866302
chr_3 g642 g642.t2 exon g642.t2.exon1 4864620 4864674
chr_3 g642 g642.t2 cds g642.t2.CDS1 4864620 4864674
chr_3 g642 g642.t2 exon g642.t2.exon2 4865592 4865704
chr_3 g642 g642.t2 cds g642.t2.CDS2 4865592 4865704
chr_3 g642 g642.t2 exon g642.t2.exon3 4865770 4865827
chr_3 g642 g642.t2 cds g642.t2.CDS3 4865770 4865827
chr_3 g642 g642.t2 exon g642.t2.exon4 4865884 4866113
chr_3 g642 g642.t2 cds g642.t2.CDS4 4865884 4866113
chr_3 g642 g642.t2 exon g642.t2.exon5 4866222 4866302
chr_3 g642 g642.t2 cds g642.t2.CDS5 4866222 4866302
chr_3 g642 g642.t2 TSS g642.t2 4866370 4866370

Sequences

>g642.t2 Gene=g642 Length=537
ATGTTTACTTCATTTAACACTGATAAAAATCGAGATATCGAAGAATTGTTGAGTGACACA
GTGTCAGCACAGGATTTAGAAAAAATCGAGAAAGTTTATATGAAAGAGTTGACAGAAAAG
AAATTTGTATCAGCAAAAACACAATTCGAGTATGCATGGTGTTTAGTAAGAAGCAAATAT
GGTGCAGATATAAATAATGGAATCAAAAATTTTGAATCACTTTGTAAAGATGATCCAGAA
AATAAGAGAGACTACATTTATTACATTGCAATTGGTTATATTCGACTTAAAGACTTACCA
ACAGCACAAAAATATGTGAAAGCTTTCCTTGAAATTGAACCAAATAATCATCAAGTGATT
TTATTAGATGAACATATTGAGACAGAAATGAGAAAAGAATTCAAAAGGGATGCAGCAGTT
GCTGGTGGTGCTTTGTTAGTTTTTGGTGGTTTACTTGGTGTTGGATTTGCTCTTGCTAAA
AAACACTATGACAAGAAGAAACAAGAAAAAGAAGAAATGAAGCCGTCAAGTGATTGA

>g642.t2 Gene=g642 Length=178
MFTSFNTDKNRDIEELLSDTVSAQDLEKIEKVYMKELTEKKFVSAKTQFEYAWCLVRSKY
GADINNGIKNFESLCKDDPENKRDYIYYIAIGYIRLKDLPTAQKYVKAFLEIEPNNHQVI
LLDEHIETEMRKEFKRDAAVAGGALLVFGGLLGVGFALAKKHYDKKKQEKEEMKPSSD

Protein features from InterProScan

Transcript Database ID Name Start End E.value
11 g642.t2 CDD cd12212 Fis1 20 133 6.7964E-32
6 g642.t2 Gene3D G3DSA:1.25.40.10 - 13 170 1.3E-41
3 g642.t2 PANTHER PTHR13247 TETRATRICOPEPTIDE REPEAT PROTEIN 11 TPR REPEAT PROTEIN 11 7 159 2.8E-31
4 g642.t2 PANTHER PTHR13247:SF0 MITOCHONDRIAL FISSION 1 PROTEIN 7 159 2.8E-31
10 g642.t2 PIRSF PIRSF008835 TPR_repeat_11_Fis1 8 161 2.5E-41
2 g642.t2 Pfam PF14852 Fis1 N-terminal tetratricopeptide repeat 47 78 1.9E-11
1 g642.t2 Pfam PF14853 Fis1 C-terminal tetratricopeptide repeat 83 133 5.0E-18
8 g642.t2 Phobius NON_CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. 1 137 -
9 g642.t2 Phobius TRANSMEMBRANE Region of a membrane-bound protein predicted to be embedded in the membrane. 138 159 -
7 g642.t2 Phobius CYTOPLASMIC_DOMAIN Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. 160 178 -
5 g642.t2 SUPERFAMILY SSF48452 TPR-like 14 161 3.95E-35
12 g642.t2 TMHMM TMhelix Region of a membrane-bound protein predicted to be embedded in the membrane. 140 159 -

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0005515 protein binding MF
GO:0000266 mitochondrial fission BP

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values