General information for g6430.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Chitotriosidase-1.
Parent gene
- The parent gene g6430 is located on chr_2: 17045379..17045789 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6430 | g6430.t1 | TSS | g6430.t1 | 17043834 | 17043834 |
| chr_2 | g6430 | g6430.t1 | isoform | g6430.t1 | 17043854 | 17045789 |
| chr_2 | g6430 | g6430.t1 | exon | g6430.t1.exon1 | 17043854 | 17043911 |
| chr_2 | g6430 | g6430.t1 | cds | g6430.t1.CDS1 | 17043854 | 17043911 |
| chr_2 | g6430 | g6430.t1 | exon | g6430.t1.exon2 | 17043968 | 17043989 |
| chr_2 | g6430 | g6430.t1 | cds | g6430.t1.CDS2 | 17043968 | 17043989 |
| chr_2 | g6430 | g6430.t1 | exon | g6430.t1.exon3 | 17044046 | 17044155 |
| chr_2 | g6430 | g6430.t1 | cds | g6430.t1.CDS3 | 17044046 | 17044155 |
| chr_2 | g6430 | g6430.t1 | exon | g6430.t1.exon4 | 17044214 | 17044399 |
| chr_2 | g6430 | g6430.t1 | cds | g6430.t1.CDS4 | 17044214 | 17044399 |
| chr_2 | g6430 | g6430.t1 | exon | g6430.t1.exon5 | 17044461 | 17045087 |
| chr_2 | g6430 | g6430.t1 | cds | g6430.t1.CDS5 | 17044461 | 17045087 |
| chr_2 | g6430 | g6430.t1 | exon | g6430.t1.exon6 | 17045149 | 17045789 |
| chr_2 | g6430 | g6430.t1 | cds | g6430.t1.CDS6 | 17045149 | 17045789 |
| chr_2 | g6430 | g6430.t1 | TTS | g6430.t1 | 17046018 | 17046018 |
Sequences
>g6430.t1 Gene=g6430 Length=1644
ATGAAATATTTGGCTATTGCAATAATTTATGCCTTTGTATCCATCACAATGGCTCAAAAA
CGGGTTGTCTGCTATTTTCCCTCATGGACAAGCATTAATCCAAATCCATCAATTTGTACA
CATTTGATTTATTCATTCCTTAATATTGGTGCTGATGGTTCTATTGTGCCATTTGATGCA
TCAGGATTTAGTCTAACTGCATTTACTGGATTACGTTCACAAAATTCAGCTTTAAAAGTC
ATTTTGGGAATTGGTGGAGCAACAGCATCAAATTTTGGACCTGTTGCAGCTAATAATGCA
TTGATAAATATATTTGCTAATAATGTTGCTCAAATCATCAATACTTATGGCTTTGATGGC
GTTGATATTGATTGGGAATATCCAAGTGTTAATGAAAATTTCATTGGACTTTTAACTGCT
GTTCGTAATGCATTGCCCAATGGAAAAATTCTTTCAATAGCTGTTGCTCCTGACTCAACA
AGAGCTAGTGCTTCTTATAATGCCCAAAGTGTTTCAGCTATAGTTGATTTTATAAATTTA
ATGACTTATGATTTCCATGGTGGTTGGGAAACTCAAACATATTCTCAAGCTCCTCTTTAT
GGTGGCACTTTAGATACTTCACCTTATTGGAAATCATTGAATGTTGATGCATGTGTTGGA
TATTGGATTTCACAAGGAGTTCCTAAAGATAAACTTGTTGTTGGTGTTCCGCTTTATGGT
AGATCATATACTCTAGTAAATTCAAATAACAATGGCGTTGCGGCACCTGCTACTGGTCAA
GCTGGAAATCCATCAACGCCAGTCTACAATCAAATTTGTTCAAATATTAAAAATAATGGA
TGGAAATCAGGCTATTATAATCCACAAGCAATTTCTTATGCATATTCAGGCAATCAATGG
GTTAGTTATGATGATTTAGTTTCGCTTACAACTAAAATCAGTTATATAAAAAGTAATAAT
TTGGGTGGAATTATGTTTTGGTCTATGGATCAAGATGATTATTTGGGAAATTGTGGAAAT
GGAAAATATCCAATGATTTCCACAGCTTATAATCTTTTGATTCAAAACAGCGTCATCGCT
CCAAATGTAACTGTAGTTACAACTACAACAACAACTACACTGCCGCCGACTACACAAGCT
GCAACACCACCATCTGGTTCATTCTCATGTCCTCTATCTTCAGGAATTTTCACTAATCCA
AGCAGTTGTACTTCATATATAAGTTGTGCAAATGGTGTCGCTTTTATTCAAGAATGTCCA
AAAGGTTTGAATTTCAATGGTTTAACAAGAGTTTGTGATTATCCATCTAATGCACCATGC
TCAAATATTCCTCCAATTATTCAAGTTACTACTACAAAACCAATTGTTAATACTAATGCA
CCTACTTTGGCACCGACAACTACCACTACTACTAAGCCGATAATAGTCACTAATCCAGCC
ACAGTAGCACCCACGTCAGCTCCATCATCTTCTAATCCTTGTGCCAATAAACTTGGAATT
ATCGCTAATCCTGCAGACTGCAATACTTTTTATAATTGTGGACCCGGTGGAGCAGGAACA
CTTATGAATTGTGGACCTGGTCTTGGCTTTAATGCAGCTATTCAAGCATGCGACTATAAG
TCAAACATTCCTGGATGTATTTAA
>g6430.t1 Gene=g6430 Length=547
MKYLAIAIIYAFVSITMAQKRVVCYFPSWTSINPNPSICTHLIYSFLNIGADGSIVPFDA
SGFSLTAFTGLRSQNSALKVILGIGGATASNFGPVAANNALINIFANNVAQIINTYGFDG
VDIDWEYPSVNENFIGLLTAVRNALPNGKILSIAVAPDSTRASASYNAQSVSAIVDFINL
MTYDFHGGWETQTYSQAPLYGGTLDTSPYWKSLNVDACVGYWISQGVPKDKLVVGVPLYG
RSYTLVNSNNNGVAAPATGQAGNPSTPVYNQICSNIKNNGWKSGYYNPQAISYAYSGNQW
VSYDDLVSLTTKISYIKSNNLGGIMFWSMDQDDYLGNCGNGKYPMISTAYNLLIQNSVIA
PNVTVVTTTTTTTLPPTTQAATPPSGSFSCPLSSGIFTNPSSCTSYISCANGVAFIQECP
KGLNFNGLTRVCDYPSNAPCSNIPPIIQVTTTKPIVNTNAPTLAPTTTTTTKPIIVTNPA
TVAPTSAPSSSNPCANKLGIIANPADCNTFYNCGPGGAGTLMNCGPGLGFNAAIQACDYK
SNIPGCI
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g6430.t1 | Coils | Coil | Coil | 547 | 547 | - |
| 12 | g6430.t1 | Gene3D | G3DSA:3.10.50.10 | - | 242 | 303 | 9.2E-18 |
| 10 | g6430.t1 | Gene3D | G3DSA:2.170.140.10 | Antimicrobial Protein | 384 | 449 | 1.9E-16 |
| 11 | g6430.t1 | Gene3D | G3DSA:2.170.140.10 | Antimicrobial Protein | 485 | 547 | 5.0E-11 |
| 4 | g6430.t1 | PANTHER | PTHR11177 | CHITINASE | 19 | 355 | 7.6E-90 |
| 3 | g6430.t1 | Pfam | PF00704 | Glycosyl hydrolases family 18 | 21 | 332 | 4.7E-78 |
| 1 | g6430.t1 | Pfam | PF01607 | Chitin binding Peritrophin-A domain | 390 | 440 | 2.6E-12 |
| 2 | g6430.t1 | Pfam | PF01607 | Chitin binding Peritrophin-A domain | 494 | 546 | 5.9E-10 |
| 15 | g6430.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 16 | g6430.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 17 | g6430.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 13 | - |
| 18 | g6430.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 14 | g6430.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 547 | - |
| 19 | g6430.t1 | ProSitePatterns | PS01095 | Chitinases family 18 active site. | 118 | 126 | - |
| 24 | g6430.t1 | ProSiteProfiles | PS50940 | Chitin-binding type-2 domain profile. | 387 | 442 | 13.888 |
| 23 | g6430.t1 | ProSiteProfiles | PS50940 | Chitin-binding type-2 domain profile. | 491 | 547 | 12.657 |
| 20 | g6430.t1 | SMART | SM00636 | 2g34 | 20 | 332 | 7.1E-106 |
| 21 | g6430.t1 | SMART | SM00494 | chi_10 | 388 | 442 | 5.4E-14 |
| 22 | g6430.t1 | SMART | SM00494 | chi_10 | 492 | 547 | 1.0E-7 |
| 8 | g6430.t1 | SUPERFAMILY | SSF51445 | (Trans)glycosidases | 20 | 353 | 2.85E-81 |
| 5 | g6430.t1 | SUPERFAMILY | SSF54556 | Chitinase insertion domain | 239 | 303 | 4.93E-13 |
| 7 | g6430.t1 | SUPERFAMILY | SSF57625 | Invertebrate chitin-binding proteins | 382 | 445 | 6.02E-16 |
| 6 | g6430.t1 | SUPERFAMILY | SSF57625 | Invertebrate chitin-binding proteins | 488 | 546 | 1.26E-11 |
| 9 | g6430.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | MF |
| GO:0008061 | chitin binding | MF |
| GO:0005975 | carbohydrate metabolic process | BP |
| GO:0005576 | extracellular region | CC |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed