General information for g644.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Cytochrome P450 9e2.
Parent gene
- The parent gene g644 is located on chr_3: 4933527..4936140 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g644 | g644.t1 | TTS | g644.t1 | 4932821 | 4932821 |
| chr_3 | g644 | g644.t1 | isoform | g644.t1 | 4932876 | 4934709 |
| chr_3 | g644 | g644.t1 | exon | g644.t1.exon1 | 4932876 | 4933061 |
| chr_3 | g644 | g644.t1 | cds | g644.t1.CDS1 | 4932876 | 4933061 |
| chr_3 | g644 | g644.t1 | exon | g644.t1.exon2 | 4933113 | 4933541 |
| chr_3 | g644 | g644.t1 | cds | g644.t1.CDS2 | 4933113 | 4933541 |
| chr_3 | g644 | g644.t1 | exon | g644.t1.exon3 | 4933665 | 4934442 |
| chr_3 | g644 | g644.t1 | cds | g644.t1.CDS3 | 4933665 | 4934442 |
| chr_3 | g644 | g644.t1 | exon | g644.t1.exon4 | 4934498 | 4934709 |
| chr_3 | g644 | g644.t1 | cds | g644.t1.CDS4 | 4934498 | 4934709 |
| chr_3 | g644 | g644.t1 | TSS | g644.t1 | 4934775 | 4934775 |
Sequences
>g644.t1 Gene=g644 Length=1605
ATGGATATTTATTTAATAATAATTTGGGTTGTCATTTTTCTAGGATATTTCATTTACAAA
AAAGCAACTAAAAATGCAAAATTCTTTGAAAAACAAGGAGTTCCATTTAAAAAACCACTG
CCGTTTGTCGGTAATTCATTACGACTTTTAATGAAGAAGGAAAATATGATAGAATCTTTA
ATGGATTATTATGAAAAATTTAAACATAGTTCTGTTTATGGAGTGTTCAGTATGAACGTA
CCTTTTTTTGTTATCACTGATCCAGAATTGATAAAACAAATTACAATTAAAGATTTTGAT
AGCTTTGTCAATCATGATAAGAGCTTTAATGAAGATGTCGATAAGTTTTCAAGCAAAATG
TTATTTACATTGATAGATGAAAAGTGGAAACATATGAGATCAGTTCTTAGTCCCATTTTC
ACAAGTTCTAAAATGAAAATGATGTTTGGAATTTTATCAGAATGTGCAAATGAATTTGTT
GAACATCATGAAGAAAAAGCAAAGCATGGAAAAGTCATTGTTAATTGTGTTGAAACTTAT
CCACGTTATACTGTTGATGGTATTTCTACTGCTGTTCTTGGGTTCAAGGGAGACTGTATT
AAGAATGAAGATAGCAAGCTCTATAAATTAGCAATGCGTATGAGAAAACCAACATTTTTG
ACAAATCTGAAACTTATGCTTTTCATCGTCTTTCGTTGGCTTTACATTAAACTTGGCTTA
CAATTGACACTCAAAGAAGTTTATGATTTCTTTTATAATGCAATCATTAAAGTAATGAAT
GAACGTGAAGAAAAAGGAATTTTCCGGCCTGATGTCGTGCAATTGCTACTTCAAGCTCGT
AAAGGACAATTGCAAAAGGAAAGTGATGTCAATGAAAAAGAATTATCAAACTTTTCTGCC
AATATTGAATATGATACTGGAGCTCAAAATAGGAAAATAACTAATTGGACTGATGAGCAT
TTTATGGCTCAAGGATTCCTGTTCTTTGCGGCTGGCTTTGACACCACTAACATTCTACTT
CAAATTACCAGTTATTGTTTAGCAAAAAATAAGGAAGTTCAAGAAGAATTAATCAGAGAA
ATTGATGATGTTTTTGAAGAACTCGATGGAAAACCAATCACATATGAAGCATTGCATAAA
ATGAAATATTTGGATATGGTTATTTCTGAAGCATTAAGATTTTGGCCTCCAGCATTTGCA
ACAACACGTCAATGTAATAAAGATTATGATATGAAATTGAAAGATGGAAAAGTTATCAAA
ATTAAAGATGGTGATCTTATTCTTCTACCAATCTATGCAATTCATCATGATGAAAGATTT
TTCCCAAATCCAGAGAAATTCGATCCACTTCGATTTAGTGATGAAAATAAAGAATCAATT
GCATTAGGATCTTACATTCCATTCGGCAGTGGACCAAGAGTTTGCATTGGAAGTCGTTTT
GCATTAATGGAAGCTAAATTGCTCATATTTAATGTTTTGAGTAAATTCACAATGGAAGTT
TGTGATCAGACACCAGAAAAACTTGAATTGATTCCATCAATGACAGAAATTACTTTCAAA
AAGGCAATTTGTGTTGAATATCGTCCACGAAAGAAGAACAATTGA
>g644.t1 Gene=g644 Length=534
MDIYLIIIWVVIFLGYFIYKKATKNAKFFEKQGVPFKKPLPFVGNSLRLLMKKENMIESL
MDYYEKFKHSSVYGVFSMNVPFFVITDPELIKQITIKDFDSFVNHDKSFNEDVDKFSSKM
LFTLIDEKWKHMRSVLSPIFTSSKMKMMFGILSECANEFVEHHEEKAKHGKVIVNCVETY
PRYTVDGISTAVLGFKGDCIKNEDSKLYKLAMRMRKPTFLTNLKLMLFIVFRWLYIKLGL
QLTLKEVYDFFYNAIIKVMNEREEKGIFRPDVVQLLLQARKGQLQKESDVNEKELSNFSA
NIEYDTGAQNRKITNWTDEHFMAQGFLFFAAGFDTTNILLQITSYCLAKNKEVQEELIRE
IDDVFEELDGKPITYEALHKMKYLDMVISEALRFWPPAFATTRQCNKDYDMKLKDGKVIK
IKDGDLILLPIYAIHHDERFFPNPEKFDPLRFSDENKESIALGSYIPFGSGPRVCIGSRF
ALMEAKLLIFNVLSKFTMEVCDQTPEKLELIPSMTEITFKKAICVEYRPRKKNN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g644.t1 | Gene3D | G3DSA:1.10.630.10 | Cytochrome p450 | 17 | 534 | 2.4E-123 |
| 2 | g644.t1 | PANTHER | PTHR24292 | CYTOCHROME P450 | 7 | 523 | 8.5E-150 |
| 7 | g644.t1 | PRINTS | PR00463 | E-class P450 group I signature | 320 | 337 | 3.3E-17 |
| 9 | g644.t1 | PRINTS | PR00385 | P450 superfamily signature | 331 | 348 | 3.6E-10 |
| 4 | g644.t1 | PRINTS | PR00463 | E-class P450 group I signature | 340 | 366 | 3.3E-17 |
| 11 | g644.t1 | PRINTS | PR00385 | P450 superfamily signature | 386 | 397 | 3.6E-10 |
| 6 | g644.t1 | PRINTS | PR00463 | E-class P450 group I signature | 430 | 454 | 3.3E-17 |
| 5 | g644.t1 | PRINTS | PR00463 | E-class P450 group I signature | 465 | 475 | 3.3E-17 |
| 8 | g644.t1 | PRINTS | PR00385 | P450 superfamily signature | 466 | 475 | 3.6E-10 |
| 3 | g644.t1 | PRINTS | PR00463 | E-class P450 group I signature | 475 | 498 | 3.3E-17 |
| 10 | g644.t1 | PRINTS | PR00385 | P450 superfamily signature | 475 | 486 | 3.6E-10 |
| 1 | g644.t1 | Pfam | PF00067 | Cytochrome P450 | 35 | 512 | 5.1E-82 |
| 16 | g644.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 18 | g644.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 22 | - |
| 14 | g644.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 23 | 218 | - |
| 17 | g644.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 219 | 236 | - |
| 15 | g644.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 237 | 534 | - |
| 21 | g644.t1 | ProSitePatterns | PS00086 | Cytochrome P450 cysteine heme-iron ligand signature. | 468 | 477 | - |
| 12 | g644.t1 | SUPERFAMILY | SSF48264 | Cytochrome P450 | 35 | 530 | 1.83E-105 |
| 20 | g644.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 19 | - |
| 19 | g644.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 219 | 236 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0020037 | heme binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0005506 | iron ion binding | MF |
| GO:0055114 | NA | NA |
KEGG
Orthology
- This transcript has been annotated with K17861 (cytochrome P450 family 13)
Pathway
This gene does not belong to any pathways.
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed