General information for g6489.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Peroxidase.
Parent gene
- The parent gene g6489 is located on chr_2: 17243932..17249327 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6489 | g6489.t3 | TSS | g6489.t3 | 17239431 | 17239431 |
| chr_2 | g6489 | g6489.t3 | isoform | g6489.t3 | 17239481 | 17240948 |
| chr_2 | g6489 | g6489.t3 | exon | g6489.t3.exon1 | 17239481 | 17240556 |
| chr_2 | g6489 | g6489.t3 | cds | g6489.t3.CDS1 | 17239481 | 17240556 |
| chr_2 | g6489 | g6489.t3 | exon | g6489.t3.exon2 | 17240624 | 17240948 |
| chr_2 | g6489 | g6489.t3 | cds | g6489.t3.CDS2 | 17240624 | 17240948 |
| chr_2 | g6489 | g6489.t3 | TTS | g6489.t3 | 17241574 | 17241574 |
Sequences
>g6489.t3 Gene=g6489 Length=1401
ATGAAAATTTTAATTCTTATCGCGGCGGCAGTAATAACTGCAAGTGTGGATGCAGATGAT
TGTGTTAGTCACACTTATCGAACATTAGATGGTTCATGCAACAATTTAAAACATCCTAAT
TGGGGTAAAGCTGGAACTCCATATGCTCGACTTTTGCCAGCACGTTATGGTGATGGAATT
TTCAGTCCACCAAAGTCAAAAACGGGTGCTGATTTGCCAAGCTCAAGACTTGTATCGACT
ACGATTTTTGATACTATTGATTCACCTGATCCCAATCATACAATTGTCACTATGCAATTT
GGTCAATTTGTTGCACATGATATGAGTTTTGGTGGGGCACCTATTCATCCATCATGCTGC
CAAGATGGAAAAATTGTCTCTCATGATCCATTATGTTATCCAATTATTGTTCCAAATGAC
GACCCAGTTCGCTCAGCCGATGGCATCGAATGTATGAATTTTCAACGCACATTAACCGAC
AGAGATAACGAACTTGATGAAAACCGAGCAAATCAACCTGCACAGCAGATAACAGTTGTG
ACAGGCTTTCTTGACCTTTCTCTCGTGTATGGAAACTCCGAGAAAGAATTAGCGCCCGTA
AGAGAATTTAATGCAGGACGTTTAAAGATGGATATCCGTAATGGAAAAGAATGGCCACCA
CATAACCCTGATGGTGATAAAATTTGTTTTGTTGAGACATCTGGAGAGACTTGCTACTTC
GGAGGCGACCCACGTCTAAATCAAAGTCCTGATTTATCAATTCTTCATATTTATTATATT
CGTGAGCATAATCGACTTGCAGGAATCTTACATGAAATGAATCCAAGTTGGTCAGATGAG
AAACTTTTCCAAGAAGCTCGTCGCATTAATATTGCTCAATATCAATATGTTGTTTATTAT
GAATGGTTGCCATTGTTATTGGGAGAACAAAATATGTTTAAAGCTAAATTGATTTATTAT
AAAGATGGTGGTGAATATGTTGATGACTATGATGAAAATGTTGATCCATCAGCACTCAAT
GATCATGCTGCTTCTGCCTTCCGTTATTTCCATTCTGAAATTGAAGGAAATTTAGAATTA
ATTTCAGAATCACGTGAATGCAAGAAATCAATGAAAATCAGTGATGTGTTCTTACGACCA
AAAATTTTGGAGCAAAATGATAACTTCGATTCTTTTGCACGTGGTATGGCAACTCAAAGA
TTCCAAAAACCTGACAAATACTTTGACATTGAAGTTAGAGAATTTCTTCTTAAGCATTTG
CGCAAATATGGTGATGACATTAGAGCAATTGATATTCAAAGAGGCAGAGATCATGGCATT
GCTAGTTATAACAGTTTCAGAGAATTCTGTGATCTTCCAAAAGCTACAAAATGGGAAGAT
TATTTGGATTTGATTTCTCAA
>g6489.t3 Gene=g6489 Length=467
MKILILIAAAVITASVDADDCVSHTYRTLDGSCNNLKHPNWGKAGTPYARLLPARYGDGI
FSPPKSKTGADLPSSRLVSTTIFDTIDSPDPNHTIVTMQFGQFVAHDMSFGGAPIHPSCC
QDGKIVSHDPLCYPIIVPNDDPVRSADGIECMNFQRTLTDRDNELDENRANQPAQQITVV
TGFLDLSLVYGNSEKELAPVREFNAGRLKMDIRNGKEWPPHNPDGDKICFVETSGETCYF
GGDPRLNQSPDLSILHIYYIREHNRLAGILHEMNPSWSDEKLFQEARRINIAQYQYVVYY
EWLPLLLGEQNMFKAKLIYYKDGGEYVDDYDENVDPSALNDHAASAFRYFHSEIEGNLEL
ISESRECKKSMKISDVFLRPKILEQNDNFDSFARGMATQRFQKPDKYFDIEVREFLLKHL
RKYGDDIRAIDIQRGRDHGIASYNSFREFCDLPKATKWEDYLDLISQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 12 | g6489.t3 | Gene3D | G3DSA:1.10.640.10 | Myeloperoxidase | 9 | 467 | 1.1E-137 |
| 2 | g6489.t3 | PANTHER | PTHR11475 | OXIDASE/PEROXIDASE | 6 | 466 | 7.8E-199 |
| 3 | g6489.t3 | PANTHER | PTHR11475:SF86 | PEROXIDASE | 6 | 466 | 7.8E-199 |
| 5 | g6489.t3 | PRINTS | PR00457 | Animal haem peroxidase signature | 50 | 61 | 5.3E-24 |
| 6 | g6489.t3 | PRINTS | PR00457 | Animal haem peroxidase signature | 96 | 111 | 5.3E-24 |
| 7 | g6489.t3 | PRINTS | PR00457 | Animal haem peroxidase signature | 239 | 257 | 5.3E-24 |
| 8 | g6489.t3 | PRINTS | PR00457 | Animal haem peroxidase signature | 257 | 277 | 5.3E-24 |
| 9 | g6489.t3 | PRINTS | PR00457 | Animal haem peroxidase signature | 282 | 308 | 5.3E-24 |
| 4 | g6489.t3 | PRINTS | PR00457 | Animal haem peroxidase signature | 344 | 354 | 5.3E-24 |
| 1 | g6489.t3 | Pfam | PF03098 | Animal haem peroxidase | 26 | 465 | 7.5E-135 |
| 14 | g6489.t3 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 18 | - |
| 15 | g6489.t3 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 2 | - |
| 16 | g6489.t3 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 3 | 13 | - |
| 17 | g6489.t3 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 14 | 18 | - |
| 13 | g6489.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 19 | 467 | - |
| 19 | g6489.t3 | ProSiteProfiles | PS50292 | Animal heme peroxidase superfamily profile. | 18 | 467 | 103.696 |
| 10 | g6489.t3 | SUPERFAMILY | SSF48113 | Heme-dependent peroxidases | 21 | 465 | 3.65E-129 |
| 11 | g6489.t3 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
| 18 | g6489.t3 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 18 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006979 | response to oxidative stress | BP |
| GO:0020037 | heme binding | MF |
| GO:0004601 | peroxidase activity | MF |
| GO:0042600 | egg chorion | CC |
| GO:0055114 | NA | NA |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed