General information for g6496.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as 3-phosphoinositide-dependent protein kinase 1.
Parent gene
- The parent gene g6496 is located on chr_2: 17284080..17285883 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6496 | g6496.t1 | TSS | g6496.t1 | 17283980 | 17283980 |
| chr_2 | g6496 | g6496.t1 | isoform | g6496.t1 | 17284080 | 17285883 |
| chr_2 | g6496 | g6496.t1 | exon | g6496.t1.exon1 | 17284080 | 17284172 |
| chr_2 | g6496 | g6496.t1 | cds | g6496.t1.CDS1 | 17284080 | 17284172 |
| chr_2 | g6496 | g6496.t1 | exon | g6496.t1.exon2 | 17284224 | 17284281 |
| chr_2 | g6496 | g6496.t1 | cds | g6496.t1.CDS2 | 17284224 | 17284281 |
| chr_2 | g6496 | g6496.t1 | exon | g6496.t1.exon3 | 17284353 | 17284855 |
| chr_2 | g6496 | g6496.t1 | cds | g6496.t1.CDS3 | 17284353 | 17284855 |
| chr_2 | g6496 | g6496.t1 | exon | g6496.t1.exon4 | 17284915 | 17284990 |
| chr_2 | g6496 | g6496.t1 | cds | g6496.t1.CDS4 | 17284915 | 17284990 |
| chr_2 | g6496 | g6496.t1 | exon | g6496.t1.exon5 | 17285053 | 17285735 |
| chr_2 | g6496 | g6496.t1 | cds | g6496.t1.CDS5 | 17285053 | 17285735 |
| chr_2 | g6496 | g6496.t1 | exon | g6496.t1.exon6 | 17285791 | 17285883 |
| chr_2 | g6496 | g6496.t1 | cds | g6496.t1.CDS6 | 17285791 | 17285883 |
| chr_2 | g6496 | g6496.t1 | TTS | g6496.t1 | 17285909 | 17285909 |
Sequences
>g6496.t1 Gene=g6496 Length=1506
ATGACTTCAAGGAGCACAAAAAAGAGCGATAACGATTTTATATTTTTAAAAATTATTGGG
GAGGCAAGAATATTAACATCCAAAATTTTAACTGGCTCATTTTCAACTGTTTATCTGTGT
CGAGATGTAGAAACTGCAAAGGAATATGCAATAAAAGCTTGCGATAAAAAATTGATTCTT
CGTGAGAAAAAACAAGAATATATCAAAAGAGAAAAGGATGCCCTTCTGAAATTAAATAAC
ACTATAGGCGTAATACAACTACTTTCAACTTTTCAATCTGACAAGTATCTCTTTTTCGTT
CTAACATATGCAAAAAATTCAGAACTACATCACTATTTGAAGCTAAAAATATTTAATTTA
GAATGTGTAAAATACTATTCAGCTCAACTTTTAATAGCAATTGAAGGCATACATAGAAAA
AATATAATTCACAGGGACCTTAAGCCCCAAAATTTGCTATTAGATTCATCTATGAAGTTA
ATAGTTGGAGATTTTGGTTCAAGTAAAATATTATCAGATCAAACTGACTCTAAAGAAGAA
CAGCTCAATATTAATAACAATAATAAAAACCAAGAAGAGAGAAACATTAGTGCTATAGGT
GAAAGCAAAAGGCGTATAGATCGAAGAGCTAGTTTTGTTGGAACAGCACTTTATGTAAGT
CCAGAAGTTCTCAAAGGAAGCAAGTCTCATTTTTCAACTGACTTATTTGCATATGGTTGC
ATCGTTTATGAAATGTGTGCACATGGACGTCCAGTGTTTGGAAGTTCTGGTGAAAATGAA
TATGTGGTTTTTCAAAAAATCCAAAATATGGATTATAAATTTCCTGAAAATTTTGATTCA
GAAGTAAAAGATTTAGTTCAAAAACTTTTAAAAATTGATCCGGAAGAGAGATTAGGTGCA
AAAGATCCAAAAGAATCAATTTATAAGTCAATTCGAGATCATGAGTTTTTTTCAACCATT
AACTTTGATACTATTTATTCACAAACTTCACCTTTAATTTTTCTCAATAATTTTGCTGAA
ATGTTGCAGCAAAATAACGTAGTGCAACCTAATGTCATTTTAGAACCGGGATTAGGAGAA
AAACAATTAAAACGTATTTTACAATCAGAACTGAATTTGACTTCATCAAACTTAACTCCA
GAAGCACTAGAAAAATTATTTAAAGAGCAAACTAAAATACAGTGGTCAGAGTTCTTAGAT
GAAAATGAATATTTAATTAAATACGGTTTTATATACAAAAGAAAAGGTTTGATACCGAGG
AAAAGAATGTTACTTTTATCACTAGCGCTTTCAACAAACACTCCTCGTTTGAGTTACATT
GATGCTAATGCAAATATTAAGAAAGGAGAGATACCTTTAGACTCAACAACAGTATGTGAA
CCCAAAAATTTTAAAACTTTTTTCATTCATACACCCAATCGCATATATTTTCTTGAAAGT
CATGAAGGAGATGCACTTAAATGGTGTGAGGCAATTGAGAAAATAAGAGATAAATATTGC
AAATAA
>g6496.t1 Gene=g6496 Length=501
MTSRSTKKSDNDFIFLKIIGEARILTSKILTGSFSTVYLCRDVETAKEYAIKACDKKLIL
REKKQEYIKREKDALLKLNNTIGVIQLLSTFQSDKYLFFVLTYAKNSELHHYLKLKIFNL
ECVKYYSAQLLIAIEGIHRKNIIHRDLKPQNLLLDSSMKLIVGDFGSSKILSDQTDSKEE
QLNINNNNKNQEERNISAIGESKRRIDRRASFVGTALYVSPEVLKGSKSHFSTDLFAYGC
IVYEMCAHGRPVFGSSGENEYVVFQKIQNMDYKFPENFDSEVKDLVQKLLKIDPEERLGA
KDPKESIYKSIRDHEFFSTINFDTIYSQTSPLIFLNNFAEMLQQNNVVQPNVILEPGLGE
KQLKRILQSELNLTSSNLTPEALEKLFKEQTKIQWSEFLDENEYLIKYGFIYKRKGLIPR
KRMLLLSLALSTNTPRLSYIDANANIKKGEIPLDSTTVCEPKNFKTFFIHTPNRIYFLES
HEGDALKWCEAIEKIRDKYCK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 10 | g6496.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 29 | 107 | 4.2E-73 |
| 12 | g6496.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 108 | 329 | 4.2E-73 |
| 11 | g6496.t1 | Gene3D | G3DSA:2.30.29.30 | - | 359 | 501 | 5.3E-35 |
| 3 | g6496.t1 | PANTHER | PTHR24356 | SERINE/THREONINE-PROTEIN KINASE | 3 | 454 | 2.9E-88 |
| 4 | g6496.t1 | PANTHER | PTHR24356:SF163 | 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED | 3 | 454 | 2.9E-88 |
| 2 | g6496.t1 | Pfam | PF00069 | Protein kinase domain | 28 | 305 | 3.9E-51 |
| 1 | g6496.t1 | Pfam | PF14593 | PH domain | 394 | 500 | 2.9E-32 |
| 9 | g6496.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 142 | 154 | - |
| 13 | g6496.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 23 | 317 | 39.566 |
| 8 | g6496.t1 | SMART | SM00220 | serkin_6 | 23 | 317 | 1.4E-64 |
| 7 | g6496.t1 | SMART | SM00233 | PH_update | 405 | 499 | 9.4E-6 |
| 6 | g6496.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 26 | 345 | 3.14E-66 |
| 5 | g6496.t1 | SUPERFAMILY | SSF50729 | PH domain-like | 311 | 499 | 5.99E-29 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K06276 (3-phosphoinositide dependent protein kinase-1)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed