General information for g6496.t6
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative 3-phosphoinositide-dependent protein kinase 1.
Parent gene
- The parent gene g6496 is located on chr_2: 17284080..17285883 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6496 | g6496.t6 | TSS | g6496.t6 | 17283980 | 17283980 |
| chr_2 | g6496 | g6496.t6 | isoform | g6496.t6 | 17284080 | 17285735 |
| chr_2 | g6496 | g6496.t6 | exon | g6496.t6.exon1 | 17284080 | 17284141 |
| chr_2 | g6496 | g6496.t6 | exon | g6496.t6.exon2 | 17284224 | 17284281 |
| chr_2 | g6496 | g6496.t6 | exon | g6496.t6.exon3 | 17284353 | 17284859 |
| chr_2 | g6496 | g6496.t6 | exon | g6496.t6.exon4 | 17284920 | 17284990 |
| chr_2 | g6496 | g6496.t6 | exon | g6496.t6.exon5 | 17285053 | 17285735 |
| chr_2 | g6496 | g6496.t6 | cds | g6496.t6.CDS1 | 17285055 | 17285735 |
| chr_2 | g6496 | g6496.t6 | TTS | g6496.t6 | 17285909 | 17285909 |
Sequences
>g6496.t6 Gene=g6496 Length=1381
ATGACTTCAAGGAGCACAAAAAAGAGCGATAACGATTTTATATTTTTAAAAATTATTGGG
GAGGCTCATTTTCAACTGTTTATCTGTGTCGAGATGTAGAAACTGCAAAGGAATATGCAA
TAAAAGCTTGCGATAAAAAATTGATTCTTCGTGAGAAAAAACAAGAATATATCAAAAGAG
AAAAGGATGCCCTTCTGAAATTAAATAACACTATAGGCGTAATACAACTACTTTCAACTT
TTCAATCTGACAAGTATCTCTTTTTCGTTCTAACATATGCAAAAAATTCAGAACTACATC
ACTATTTGAAGCTAAAAATATTTAATTTAGAATGTGTAAAATACTATTCAGCTCAACTTT
TAATAGCAATTGAAGGCATACATAGAAAAAATATAATTCACAGGGACCTTAAGCCCCAAA
ATTTGCTATTAGATTCATCTATGAAGTTAATAGTTGGAGATTTTGGTTCAAGTAAAATAT
TATCAGATCAAACTGACTCTAAAGAAGAACAGCTCAATATTAATAACAATAATAAAAACC
AAGAAGAGAGAAACATTAGTGCTATAGGTGAAAGCAAAAGGCGTATAGATCGAAGAGCTA
GTTTTGTTGGAACAGCACTTTATGTAATCCAGAAGTTCTCAAAGGAAGCAAGTCTCATTT
TTCAACTGACTTATTTGCATATGGTTGCATCGTTTATGAAATGTGTGCACATGGACGTCC
AGTGTTTGGAAGTTCTGGTGAAAATGAATATGTGGTTTTTCAAAAAATCCAAAATATGGA
TTATAAATTTCCTGAAAATTTTGATTCAGAAGTAAAAGATTTAGTTCAAAAACTTTTAAA
AATTGATCCGGAAGAGAGATTAGGTGCAAAAGATCCAAAAGAATCAATTTATAAGTCAAT
TCGAGATCATGAGTTTTTTTCAACCATTAACTTTGATACTATTTATTCACAAACTTCACC
TTTAATTTTTCTCAATAATTTTGCTGAAATGTTGCAGCAAAATAACGTAGTGCAACCTAA
TGTCATTTTAGAACCGGGATTAGGAGAAAAACAATTAAAACGTATTTTACAATCAGAACT
GAATTTGACTTCATCAAACTTAACTCCAGAAGCACTAGAAAAATTATTTAAAGAGCAAAC
TAAAATACAGTGGTCAGAGTTCTTAGATGAAAATGAATATTTAATTAAATACGGTTTTAT
ATACAAAAGAAAAGGTTTGATACCGAGGAAAAGAATGTTACTTTTATCACTAGCGCTTTC
AACAAACACTCCTCGTTTGAGTTACATTGATGCTAATGCAAATATTAAGAAAGGAGAGAT
ACCTTTAGACTCAACAACAGTATGTGAACCCAAAAATTTTAAAACTTTTTTCATTCATAC
A
>g6496.t6 Gene=g6496 Length=227
MCAHGRPVFGSSGENEYVVFQKIQNMDYKFPENFDSEVKDLVQKLLKIDPEERLGAKDPK
ESIYKSIRDHEFFSTINFDTIYSQTSPLIFLNNFAEMLQQNNVVQPNVILEPGLGEKQLK
RILQSELNLTSSNLTPEALEKLFKEQTKIQWSEFLDENEYLIKYGFIYKRKGLIPRKRML
LLSLALSTNTPRLSYIDANANIKKGEIPLDSTTVCEPKNFKTFFIHT
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g6496.t6 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 4 | 86 | 0.000 |
| 6 | g6496.t6 | Gene3D | G3DSA:2.30.29.30 | - | 99 | 227 | 0.000 |
| 2 | g6496.t6 | PANTHER | PTHR24356 | SERINE/THREONINE-PROTEIN KINASE | 5 | 204 | 0.000 |
| 3 | g6496.t6 | PANTHER | PTHR24356:SF163 | 3-PHOSPHOINOSITIDE-DEPENDENT PROTEIN KINASE 1-RELATED | 5 | 204 | 0.000 |
| 1 | g6496.t6 | Pfam | PF14593 | PH domain | 150 | 227 | 0.000 |
| 8 | g6496.t6 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1 | 73 | 8.641 |
| 5 | g6496.t6 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 3 | 101 | 0.000 |
| 4 | g6496.t6 | SUPERFAMILY | SSF50729 | PH domain-like | 67 | 227 | 0.000 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
