General information for g652.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Mitogen-activated protein kinase kinase kinase 4.
Parent gene
- The parent gene g652 is located on chr_3: 5010815..5011457 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g652 | g652.t1 | TSS | g652.t1 | 5004502 | 5004502 |
| chr_3 | g652 | g652.t1 | isoform | g652.t1 | 5004586 | 5010815 |
| chr_3 | g652 | g652.t1 | exon | g652.t1.exon1 | 5004586 | 5004653 |
| chr_3 | g652 | g652.t1 | cds | g652.t1.CDS1 | 5004586 | 5004653 |
| chr_3 | g652 | g652.t1 | exon | g652.t1.exon2 | 5005245 | 5005394 |
| chr_3 | g652 | g652.t1 | cds | g652.t1.CDS2 | 5005245 | 5005394 |
| chr_3 | g652 | g652.t1 | exon | g652.t1.exon3 | 5005610 | 5006394 |
| chr_3 | g652 | g652.t1 | cds | g652.t1.CDS3 | 5005610 | 5006394 |
| chr_3 | g652 | g652.t1 | exon | g652.t1.exon4 | 5006706 | 5008737 |
| chr_3 | g652 | g652.t1 | cds | g652.t1.CDS4 | 5006706 | 5008737 |
| chr_3 | g652 | g652.t1 | exon | g652.t1.exon5 | 5009108 | 5009882 |
| chr_3 | g652 | g652.t1 | cds | g652.t1.CDS5 | 5009108 | 5009882 |
| chr_3 | g652 | g652.t1 | exon | g652.t1.exon6 | 5009952 | 5010129 |
| chr_3 | g652 | g652.t1 | cds | g652.t1.CDS6 | 5009952 | 5010129 |
| chr_3 | g652 | g652.t1 | exon | g652.t1.exon7 | 5010201 | 5010418 |
| chr_3 | g652 | g652.t1 | cds | g652.t1.CDS7 | 5010201 | 5010418 |
| chr_3 | g652 | g652.t1 | exon | g652.t1.exon8 | 5010483 | 5010665 |
| chr_3 | g652 | g652.t1 | cds | g652.t1.CDS8 | 5010483 | 5010665 |
| chr_3 | g652 | g652.t1 | exon | g652.t1.exon9 | 5010759 | 5010815 |
| chr_3 | g652 | g652.t1 | cds | g652.t1.CDS9 | 5010759 | 5010815 |
| chr_3 | g652 | g652.t1 | TTS | g652.t1 | 5010931 | 5010931 |
Sequences
>g652.t1 Gene=g652 Length=4446
ATGGCGAGTGGAGCTACTATAGATGACAAGAAAGTCTCATTTGTCATAGATTCGGATGAA
GATAAAAGGAATTCAACGATACCTGAAGAGGACGAAGAGGAAGAATTTTTCGATGAAGAT
TTTGGACAATTGCCTGAATTAATTGACCTTTATAGTTCTACACCACCACGAACGCGTATT
AAGCTTAAAAATCGCATTCGTGAAAACAGACTCAAAATGGAAACAGTTGCGAATGTCAAG
ATGAGATCGCGTGCGATAAGATCCAAAGCTGATCGACGAAACACACTTGATTGCGCACTT
CTAAATCAAATGATAATTGAGCCAAGTATTGATGAAAAAGCACGAAGTGATAAACGATCA
ATTCAACAACAGAGAGAATATGAAAGAGATCAAAAAAATGCAATGACTGGAAATAATATG
ACATTTCGTAATAGCAATCGACAGTCGTTAAATTTTGATGATCATCATACAAACAATGTC
ACACATAGTAATGTTAACAATGAGCCAAATAATGAACCTACAGAATCAAAATGTATTGAA
TCATCAAATCGATTTATGAGTGTTCGATCGCGACCTGTTGGTTGTCGAATTTCAGCACCA
GCTTCTGCTTTCCTCAATCTTTCATTCAAAATACCATCAGGACCCACCGATGCAGGTCCA
CAAACGAGAAGAGAATTTCACGAGACTTTTTCTAATTTAATTAAATTGGGGTCAAATGAT
AAATCTGATAGATGTGCCAAGAGCATGATTATTTCTCGTGAAGAACAATTATGGCAGACT
GAAATTAAAGATATGATTTGGCTTGAACTACAAGCAAATATGGCTGATAGAACACTCGAG
CAACAAGATAAATATCTTTTTACAGCACGACAAAATGTCGGCGAATTATTACGTGAAATT
ATGACTTATAGATTTGTGAGAAATTTCAATCGACAAGATAGTGGTGGAAAATCATCTGAT
TCTGGTATATCATCGATGGCTAGTACAAGTGATAGCACTTCAAATGTTTCAACAGGTTTT
TGTTTTGGATGTCTATCAATGTATTGTAAAGACTGTTTAGATGCTCAAAATTCTGCATTG
AGACAAGTCGAAAGTTTGCTCACTCGTCTCGAAGCTGCTGAAAATTTATTTCCATCAAAC
AAAGCATTTGGTGCGCATTTTCAACTCTACAAATCAGAGGAATTTGTAAATCGTGTCAAA
TGTATGTGTTTGTGGTATAACATGACACGACATCATCGTTTGAAATTGACAATATTGGGA
AAATTCCTAGCAAAACTACAAGGTAAAGAATACAAATGGCCATTAGTTGATTCAGATAGC
AGCAGTGGTGCATCATCAATACAAGAAGAAACAGACTCAGCTCGTGGTACAGCAAGTGCT
GAATGTAAAACAATAAAAGATTGTCCAAAAGTTCAATTTTTAGATGTCGATCAAGTTGAT
CAAGCTAGTGCAACTGATAGTGCAAATAGTGAAGAGTCAAATAGAAATGATAATAGTACC
AATAGCATATTTCATCCGATGTATGAATATGGAAAAATTCTAAATTCCTTAACTGATTTA
AGTTGGAACAAACTTGGCTCAAGTGAAGATGTACATAAAAAATTCCCAACTTCACCATAT
CGTAAATTCATTGAAAATGTACTTAAAAGTCGTGGACTTGGTAAATCACTCACGTTTCTT
CACAGACTGCATAATGTTGTCCTTCGAAAGGCTCATTTGACACTTGAAAAACCTGGAACT
GAAGATGAAAATGAATTTGAGCAATTTGATGAAGATGAAATTCCAAATATTGAGCCACCA
ATGGATGCTGAAGAGGCTAATGAACTCCGTCGTTATGGCGTATGGAGCGATGAATATAAA
CAATTATCACTGCCAACATACATCTCAGCTTTTATTTTTCTATCACTCATACCATTAGAA
GTTGTTCAAGAATTCCTCAAAATGAAATTAGAAACTAAACCAGTTCAACCAAATCCATTG
AGTCTTGAACAGTTGATTAAAGAACTCAAAGAAGGTCTTACACTTGCACTTATTCATCGT
GACCGTTTCCATAAGCATATCACATCAGCTTTAATCGACCGTGATCAGGATCTTGAAACA
TATATGCAACATATTGACGAATTTGATGGAACATTAAAGAAAATTTTTGAGCTTTATCTT
GAATACGTAACACAATGGATTCTTACAGGAATTCCAGAACCACAACGCAAGAATGCACTA
GAAACAGAATGGAAATTTACCAAACTTGTGTGTCCTATGATTCATGATGAGCATGCAGTT
GCAGCGCAGAAATTTTGTACAATAATTGAGTCACTATTAAATGAGATAGGAAGTCGATTG
ACGACAAAAGCTGATGAATTAGAACATATGAATTTTTCACACGATGTTTCAGCTGAAAAA
AGTATTAAAAAAGATGAAATTGTTGAAAATGGAAATGCAAGTGATGGAAATAATAATAAT
GATGATGATGAAGATGAAGATCCGTCCTCAATATTTAAGAACAAATTATTAACAATGTGT
AGAGAAATTCAGCAGTTGTTTACAGATGAAAGAGAAAAATGTATGAAATTAATGTTATTT
ACAAAATGTTTCCTCAAAGACATAGAAAAAGATGATTTTCATCGTGAACATAATTGTCCG
AATGACAGTACAGTTTGTTTAAGACATAGTACAAGCATGTGTACAGAAGTATTAGAAGCT
ATTAATGGACTTAAATCTAAAACTCTTGAATTACGTGAACAATTAACAACAACCTTACAA
CGAGTACAAGAAAGAAGTGATGTGAAACATTTAATGGACATGGATGAAATGGATCGTCAA
ACAGTGCTTACAAGATGTAAAGAGATTTTACACGTTGGGTATAAATTTGGATTCGAATAC
CACAAAGACTTATCAAAACTTTTTGAGACTGTTTCATCGCCATCAAGAAATTCAAAATGC
GAACTAGGTCTTGCAAAAGCAATTGTTGGTTTTGCAAAATGTTGGATGAATTTTGTTATG
GAAAGATGTGAAAGAGGAAGAGGACTAAGACCAAGATGGGCAGCAACTGGTTTAGATTTC
TTAATTATTGCCTGCGATCCTCACAATACAGAACAAATTGATGATAAGCAATTCGAAGAA
CTCAAAAGTTCAATGGATAGATGCATTTCTCATGTTATTGGTTCAATTCATGAGCCAGAA
AGAGTAAGAAAATCTCCTCGATCAAGAAAATCTTCACCTGCACCACGTCGTAGAACGCCA
ACACGATCTTCTATTTCATATACGCCGACAGCACAAAAAGTGCTTCAACAACAGTTGAGT
CTACAATCTGAAAAAAATCGTCTCACACCCTCGTCTCCTGATCCACGGGATTTTCCAAGC
GAATTCATTCGCAAACAAATATCTTATGATAATGTTAATGACGTTAGCATTAGAGTTCCA
CTTAGTCCATTAAGTCCACATGCAACACCTGAATTACGACAAGTTAGAATTCGTGACGCT
GTCAATCGACTGGATCTTCAATTAGAGAACAATTTACGAGACAAAAATCTTATAGGAACT
GTAAAAGAATTAAATTCATGTGATAAAGTTGTTATACGTGCACGAAGTGTTAATTTTAGT
TGGCATCGTGGTATAAAAATAGGTCAAGGACGATTTGGAAAAGTTTACACAGCTGTAAAT
AATTCAAATGGTGAATTAATGGCGATGAAAGAACTTCCAATTCAAGCAGGCGAAACGGGA
ACAATTCGACGAGTTGCTGAAGAATTGAAAATATTTGAAGGAATTCAACATAAACATTTA
GTCAGATATTACGGCGTTGAAATTCACAGGGAAGAGCTTTTGATTTTCATGGAGTTGTGT
GCTGAAGGAACACTTGAGTCATTATGTGAGATTTCAGGAGGTTTACATGAAGCACTTATT
CGAAGATACACTACACAATTACTTCTTGCTGTTGAAGAGCTTCATAAGAATGGTATTGTA
CATCGTGATATAAAATGTGCCAACATATTTCTTACAAATGAAGGAAATTGTCTAAAACTC
GGAGATTTTGGTTCAGCGATAAAAATTCAATTCCGTAGTACTATGCCAGGTGAATTGAAA
GGCTATGTTGGCACACAAGCTTATATGGCGCCTGAAGTATTTACAAAGAATAACACTGAA
GGTCATGGAAGAGCGGCAGACATATGGTCAGTTGGTTGCTGCGTCATTGAGATGGCATCT
GGAAAACGGCCCTGGAGTCAATTTGAAAGCAATTTCCAAATAATGTTTAAAGTCGGAATG
GGTGAAACTCCTGATATACCATCAAATTTATCAGAAGAAGGCATTGATTTTGTAGAATGC
TGTCTTATTCATGATCCTAAAGAACGATGGACTGCTAGTGAACTTCTACAACACTCAAAC
TTTTGTAAAGTTTCATTAGACAATGATTGTAGCTGTGATGATAACAAGAATAAGTCAAAG
AAGTAA
>g652.t1 Gene=g652 Length=1481
MASGATIDDKKVSFVIDSDEDKRNSTIPEEDEEEEFFDEDFGQLPELIDLYSSTPPRTRI
KLKNRIRENRLKMETVANVKMRSRAIRSKADRRNTLDCALLNQMIIEPSIDEKARSDKRS
IQQQREYERDQKNAMTGNNMTFRNSNRQSLNFDDHHTNNVTHSNVNNEPNNEPTESKCIE
SSNRFMSVRSRPVGCRISAPASAFLNLSFKIPSGPTDAGPQTRREFHETFSNLIKLGSND
KSDRCAKSMIISREEQLWQTEIKDMIWLELQANMADRTLEQQDKYLFTARQNVGELLREI
MTYRFVRNFNRQDSGGKSSDSGISSMASTSDSTSNVSTGFCFGCLSMYCKDCLDAQNSAL
RQVESLLTRLEAAENLFPSNKAFGAHFQLYKSEEFVNRVKCMCLWYNMTRHHRLKLTILG
KFLAKLQGKEYKWPLVDSDSSSGASSIQEETDSARGTASAECKTIKDCPKVQFLDVDQVD
QASATDSANSEESNRNDNSTNSIFHPMYEYGKILNSLTDLSWNKLGSSEDVHKKFPTSPY
RKFIENVLKSRGLGKSLTFLHRLHNVVLRKAHLTLEKPGTEDENEFEQFDEDEIPNIEPP
MDAEEANELRRYGVWSDEYKQLSLPTYISAFIFLSLIPLEVVQEFLKMKLETKPVQPNPL
SLEQLIKELKEGLTLALIHRDRFHKHITSALIDRDQDLETYMQHIDEFDGTLKKIFELYL
EYVTQWILTGIPEPQRKNALETEWKFTKLVCPMIHDEHAVAAQKFCTIIESLLNEIGSRL
TTKADELEHMNFSHDVSAEKSIKKDEIVENGNASDGNNNNDDDEDEDPSSIFKNKLLTMC
REIQQLFTDEREKCMKLMLFTKCFLKDIEKDDFHREHNCPNDSTVCLRHSTSMCTEVLEA
INGLKSKTLELREQLTTTLQRVQERSDVKHLMDMDEMDRQTVLTRCKEILHVGYKFGFEY
HKDLSKLFETVSSPSRNSKCELGLAKAIVGFAKCWMNFVMERCERGRGLRPRWAATGLDF
LIIACDPHNTEQIDDKQFEELKSSMDRCISHVIGSIHEPERVRKSPRSRKSSPAPRRRTP
TRSSISYTPTAQKVLQQQLSLQSEKNRLTPSSPDPRDFPSEFIRKQISYDNVNDVSIRVP
LSPLSPHATPELRQVRIRDAVNRLDLQLENNLRDKNLIGTVKELNSCDKVVIRARSVNFS
WHRGIKIGQGRFGKVYTAVNNSNGELMAMKELPIQAGETGTIRRVAEELKIFEGIQHKHL
VRYYGVEIHREELLIFMELCAEGTLESLCEISGGLHEALIRRYTTQLLLAVEELHKNGIV
HRDIKCANIFLTNEGNCLKLGDFGSAIKIQFRSTMPGELKGYVGTQAYMAPEVFTKNNTE
GHGRAADIWSVGCCVIEMASGKRPWSQFESNFQIMFKVGMGETPDIPSNLSEEGIDFVEC
CLIHDPKERWTASELLQHSNFCKVSLDNDCSCDDNKNKSKK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g652.t1 | CDD | cd06626 | STKc_MEKK4 | 1201 | 1458 | 2.16411E-147 |
| 8 | g652.t1 | Coils | Coil | Coil | 353 | 376 | - |
| 7 | g652.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 1200 | 1481 | 9.2E-75 |
| 14 | g652.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 111 | 132 | - |
| 16 | g652.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 111 | 139 | - |
| 15 | g652.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1059 | 1088 | - |
| 13 | g652.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1100 | 1119 | - |
| 3 | g652.t1 | PANTHER | PTHR48016:SF32 | MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 4 | 113 | 803 | 4.7E-118 |
| 5 | g652.t1 | PANTHER | PTHR48016 | MAP KINASE KINASE KINASE SSK2-RELATED-RELATED | 113 | 803 | 4.7E-118 |
| 2 | g652.t1 | PANTHER | PTHR48016:SF32 | MITOGEN-ACTIVATED PROTEIN KINASE KINASE KINASE 4 | 1188 | 1467 | 4.7E-118 |
| 4 | g652.t1 | PANTHER | PTHR48016 | MAP KINASE KINASE KINASE SSK2-RELATED-RELATED | 1188 | 1467 | 4.7E-118 |
| 1 | g652.t1 | Pfam | PF00069 | Protein kinase domain | 1203 | 1460 | 4.3E-63 |
| 12 | g652.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 1207 | 1230 | - |
| 11 | g652.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 1319 | 1331 | - |
| 17 | g652.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 1201 | 1461 | 45.248 |
| 10 | g652.t1 | SMART | SM00220 | serkin_6 | 1201 | 1461 | 5.9E-88 |
| 6 | g652.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 1206 | 1466 | 4.59E-74 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K04428 (mitogen-activated protein kinase kinase kinase 4)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
