General information for g655.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Prolyl 3-hydroxylase sudestada1.
Parent gene
- The parent gene g655 is located on chr_3: 5027087..5030818 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g655 | g655.t1 | TSS | g655.t1 | 5024045 | 5024045 |
| chr_3 | g655 | g655.t1 | isoform | g655.t1 | 5024116 | 5027706 |
| chr_3 | g655 | g655.t1 | exon | g655.t1.exon1 | 5024116 | 5024131 |
| chr_3 | g655 | g655.t1 | cds | g655.t1.CDS1 | 5024116 | 5024131 |
| chr_3 | g655 | g655.t1 | exon | g655.t1.exon2 | 5024256 | 5025842 |
| chr_3 | g655 | g655.t1 | cds | g655.t1.CDS2 | 5024256 | 5025842 |
| chr_3 | g655 | g655.t1 | exon | g655.t1.exon3 | 5025973 | 5026048 |
| chr_3 | g655 | g655.t1 | cds | g655.t1.CDS3 | 5025973 | 5026048 |
| chr_3 | g655 | g655.t1 | exon | g655.t1.exon4 | 5026112 | 5026470 |
| chr_3 | g655 | g655.t1 | cds | g655.t1.CDS4 | 5026112 | 5026470 |
| chr_3 | g655 | g655.t1 | exon | g655.t1.exon5 | 5026529 | 5027706 |
| chr_3 | g655 | g655.t1 | cds | g655.t1.CDS5 | 5026529 | 5027706 |
| chr_3 | g655 | g655.t1 | TTS | g655.t1 | 5027804 | 5027804 |
Sequences
>g655.t1 Gene=g655 Length=3216
ATGGTCAAATTAGATGGCAAAAGGAAAATTTTAAACGAGAATGGAAAACATGATGACCAC
AATGATGGCTGGAAAGTTACAAATTCATATGAAGGACCGTCAAGGAAAATGTCTAAATTG
GATAAATGGATCGAGAGTGATTTTGATGACCAAAAAGAAAAATCAACAATTCTTCAAAAT
TCAGATGAAGAAATTACATTAAATCAAACAATTGTAAGCGATGAGTTTATGAAAGAATTT
GCAAAATGCTTTGATGAAAAGAAATCATTCAAACTCAAAAACAAAATCAAATTATACAAT
GAACCTTTTCAAGCAGCAGTCATCAAAAATTTTCTATCTGATGAGTCAGTAATTGAAAAG
TTAGTAGAAGAAATTGAGCAACAAGAGTGGACAAGAAAACAAATGGATCTATATGAATTT
TATCAAACAACTGATTTAGCTAACATTAAAAGTCCATATCTATCAAAATTCTATAATTTC
ATAAACAACAATGTACGTGAGTGGATGGAAAAACTAACAGAAATGAAATTTCAACGTGCT
TCAGCCTCTTGCTCAATGTATAATTGTGGTGACTTTCTTCTTTCGCATGATGATTTATTG
AGTGACCGACTTGTGGCCTTTGTTTTTTATCTCTCACCTTGGGCGAACAAGAAAACATGG
AATGAGTCGATGGGAGGTGCTCTTGAATTATTTACATGTGACAATGATGGCCAACCGAAA
TTTCCACCAGTTGAAAAAATCTTTCCATCAAGCAATCAACTTGCATTCTTTAAAGTCGAA
AAGAGATCTCATCATCAAGTTGGTGAAGTGTTAACAAAAGATTATCCTCGATTGACAATT
AATGGATGGTTTCATGGTTTTAAGGATAACATAGATTATGACGCAGATGCTGTTAAAATT
AAAAGACCAAATGTGCCAATTTTTAAAACTCCAAATAATGATTCTTACGAAATTGAAAAC
ATTATTAATCGAAATTATCTAAAAGATAAAATTAAGAAAGAAATTCAAAAGCAAATTGAA
GAGAATTCTGAAGCTGGTTTGGGTGAGTTTTTGAAAAATCAATTTTTAATTGAAATTGAG
AATGAACTCAAAGGGCCGAAATTAAAGTGGATTCAAAAAGGACCAGCAAACCAACAAAAT
TATGAAATTTTAGATAAAAACTCTTTACCAAAATCTTCACATTTAGGCAAACTTATTGAT
CTTGTTGCCTCTAAGCAATTTTTTAAACTTTTATATGACTACACTGAACTCGATTTGCAT
GGGAAAAATGCAAAAAATCCAAAGTGTACAGTAGAATTTCAAAGATGGAAACGAGGTTGT
TACACATTAATAAATGATCCTTCAACATATAACTACGACTGTCTTGACTTACTATATTTT
ATTGGAACTAATGAAAGCATTGGTACAACAACTTATTTGACACCAGAAGGAAAGCAAGAA
GATGAGTCTGTCGTCTCTTCAGAATTTGAAAATGGACAAGATGATGATGATGATTCTGTA
CTTTTAACCATCTATCCACAAAATAATTTTGTTAATTTAGTATATCGATCAAGTGGAACT
ACAAGATTCATGAAATATTGCTATAAATCTGCAATCATAGAGAATGAGTATTATCGATTG
CTTTCAACAATTTCGTACATTTTAGTAATTTTTGTAATAGGTGTACCAATGTGGTGGAAA
ACCACAGAAATACATAGAGCAGATCTCCCATCAAATGATATTGAAGCACTTGATGACAAA
CCAATTACATTTAGTATTGAAATAGGTATTTTTATTAATAATGATGAGAGACAAAAAGAG
TTCATTGATGAGTTGGAAGTATACTTTAGAAACAATGATATATTTGGAGCAAAATTAATT
CCAATCAAACTTACCGATATGGAATTTGTAAATATCAAAACTCCTGCCAAATTAGAACAA
ATTTTAAATAAGCATTATGAAATTAAAGCCGGTAATTTGCTCGTTGCTGAATGGAAAAAC
TTGGAAGAAGAAATTTTAGTTACAAATGAAAGAACAATTTTTATTTCACCAAAAGCATCA
TCAGATAAAATTTATCAAGTAGTTCGTTCTTGGATTGTTCGTGACTATAAAATTCGATCA
ATTTTGGGACAAATAAAATCATTTGATGGTAAGCAAATTCGTCATAAATATCCACCGCCA
TCACCTGAATATGAAATAATGATTTCTGTCCTCAATCCAAATCCTGATTTACAAACGCTT
CATTGGAATGTGAGAAAAGCAACTGAAATGTATTTACAACCATTTCTCAATGAGCTCAGT
GATTTATCAAATTTTACTCTTAAATCTCAATGGAAGTATCAAGTTGTATTGAAATATGAT
AACAAGCAAATTAAAGATGAATCAAAAATGGGCAGACATTTTGCTATGGAAAAAGATTCT
CTACCTCAAATTATCAATTCCATTGAAAGAAAATTAGGAACAGACGTTGTTAACAAGCCA
TGCATTCATTTAGTTGTTTATGCACCTCCATGCAAATTTGCACCGGTCTATGTTTATAAA
GATGGTTCACGCATATCTGATCATTCATTTGATTCATTTATTTCTGCAAAATGGGGAGGA
ATTGTTATAGCAAATCCTTCAGATGCTGTCTGTTCATCACAATCGGATGAATCAAATAAT
CCACAAGAGATTTACTTACACTCTCATGAAGTTATGGATTTTGTCTTGTATCAATTGAGA
AAAATTTTTGAACTACAAGTTGAAATACCGTTGAATTATGCATCAATGGTTAGTTTAGAG
CAAATTACACCGAGATTATGGGAAAAAGATATGTATTTAAGAAATGGTGCTATACATTTG
ATCGCCTCAGCTTCATCAACCCTTAAATCATTGATTAAATTGCTAGATGATATTAAAAAT
ATTGTCATTGGTGACCATGTTGGAGCAGAAATTCATACAGCTTATAAGAATGTTGTTCTA
GCAAAAGAGTTTTTAGCTACAAACGATATTCATAATGCAGTGAAGCATGCACGCCTTTCT
TTTATTGCTGCCGAAAAGGCATTCAATGATCCTAGTTTGCTCGAGTTGTTATATTTTCCA
GAAGAACAAAAATATGCTATATACATTCCTTTATATTTGCCCATTATGATTCCTGTATTC
TTTAGCTTCAAAGCTATCAAAAAATATTTTACAAAATCTAAGGAGGAAGAAACTGTTGAA
AGTAATGATAATGTAGAGTCAAAAAAGGATGATTAA
>g655.t1 Gene=g655 Length=1071
MVKLDGKRKILNENGKHDDHNDGWKVTNSYEGPSRKMSKLDKWIESDFDDQKEKSTILQN
SDEEITLNQTIVSDEFMKEFAKCFDEKKSFKLKNKIKLYNEPFQAAVIKNFLSDESVIEK
LVEEIEQQEWTRKQMDLYEFYQTTDLANIKSPYLSKFYNFINNNVREWMEKLTEMKFQRA
SASCSMYNCGDFLLSHDDLLSDRLVAFVFYLSPWANKKTWNESMGGALELFTCDNDGQPK
FPPVEKIFPSSNQLAFFKVEKRSHHQVGEVLTKDYPRLTINGWFHGFKDNIDYDADAVKI
KRPNVPIFKTPNNDSYEIENIINRNYLKDKIKKEIQKQIEENSEAGLGEFLKNQFLIEIE
NELKGPKLKWIQKGPANQQNYEILDKNSLPKSSHLGKLIDLVASKQFFKLLYDYTELDLH
GKNAKNPKCTVEFQRWKRGCYTLINDPSTYNYDCLDLLYFIGTNESIGTTTYLTPEGKQE
DESVVSSEFENGQDDDDDSVLLTIYPQNNFVNLVYRSSGTTRFMKYCYKSAIIENEYYRL
LSTISYILVIFVIGVPMWWKTTEIHRADLPSNDIEALDDKPITFSIEIGIFINNDERQKE
FIDELEVYFRNNDIFGAKLIPIKLTDMEFVNIKTPAKLEQILNKHYEIKAGNLLVAEWKN
LEEEILVTNERTIFISPKASSDKIYQVVRSWIVRDYKIRSILGQIKSFDGKQIRHKYPPP
SPEYEIMISVLNPNPDLQTLHWNVRKATEMYLQPFLNELSDLSNFTLKSQWKYQVVLKYD
NKQIKDESKMGRHFAMEKDSLPQIINSIERKLGTDVVNKPCIHLVVYAPPCKFAPVYVYK
DGSRISDHSFDSFISAKWGGIVIANPSDAVCSSQSDESNNPQEIYLHSHEVMDFVLYQLR
KIFELQVEIPLNYASMVSLEQITPRLWEKDMYLRNGAIHLIASASSTLKSLIKLLDDIKN
IVIGDHVGAEIHTAYKNVVLAKEFLATNDIHNAVKHARLSFIAAEKAFNDPSLLELLYFP
EEQKYAIYIPLYLPIMIPVFFSFKAIKKYFTKSKEEETVESNDNVESKKDD
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g655.t1 | Gene3D | G3DSA:2.60.120.620 | q2cbj1_9rhob like domain | 66 | 288 | 6.8E-65 |
| 5 | g655.t1 | Gene3D | G3DSA:2.60.120.620 | q2cbj1_9rhob like domain | 299 | 546 | 1.5E-49 |
| 14 | g655.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 30 | - |
| 15 | g655.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 1 | 25 | - |
| 4 | g655.t1 | PANTHER | PTHR12117 | HISTONE ACETYLTRANSFERASE COMPLEX | 50 | 538 | 6.8E-105 |
| 1 | g655.t1 | Pfam | PF13661 | 2OG-Fe(II) oxygenase superfamily | 183 | 285 | 2.8E-24 |
| 2 | g655.t1 | Pfam | PF10637 | Oxoglutarate and iron-dependent oxygenase degradation C-term | 319 | 531 | 1.5E-28 |
| 3 | g655.t1 | Pfam | PF10510 | Phosphatidylinositol-glycan biosynthesis class S protein | 545 | 1050 | 1.7E-106 |
| 8 | g655.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1 | 536 | - |
| 11 | g655.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 537 | 559 | - |
| 9 | g655.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 560 | 1024 | - |
| 10 | g655.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1025 | 1046 | - |
| 7 | g655.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1047 | 1071 | - |
| 16 | g655.t1 | ProSiteProfiles | PS51471 | Fe(2+) 2-oxoglutarate dioxygenase domain profile. | 176 | 286 | 8.217 |
| 13 | g655.t1 | SMART | SM00702 | p4hc | 103 | 285 | 2.2E-22 |
| 12 | g655.t1 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1026 | 1043 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005506 | iron ion binding | MF |
| GO:0016255 | attachment of GPI anchor to protein | BP |
| GO:0055114 | NA | NA |
| GO:0031418 | L-ascorbic acid binding | MF |
| GO:0016705 | oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen | MF |
| GO:0042765 | GPI-anchor transamidase complex | CC |
| GO:0016706 | 2-oxoglutarate-dependent dioxygenase activity | MF |
| GO:0016491 | oxidoreductase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K05291 (GPI-anchor transamidase subunit S)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
