General information for g6582.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as STE20-like serine/threonine-protein kinase.
Parent gene
- The parent gene g6582 is located on chr_2: 17706613..17709844 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6582 | g6582.t1 | isoform | g6582.t1 | 17706613 | 17709844 |
| chr_2 | g6582 | g6582.t1 | exon | g6582.t1.exon1 | 17706613 | 17706936 |
| chr_2 | g6582 | g6582.t1 | cds | g6582.t1.CDS1 | 17706613 | 17706936 |
| chr_2 | g6582 | g6582.t1 | exon | g6582.t1.exon2 | 17707001 | 17707263 |
| chr_2 | g6582 | g6582.t1 | cds | g6582.t1.CDS2 | 17707001 | 17707263 |
| chr_2 | g6582 | g6582.t1 | exon | g6582.t1.exon3 | 17707321 | 17707711 |
| chr_2 | g6582 | g6582.t1 | cds | g6582.t1.CDS3 | 17707321 | 17707711 |
| chr_2 | g6582 | g6582.t1 | exon | g6582.t1.exon4 | 17708370 | 17708621 |
| chr_2 | g6582 | g6582.t1 | cds | g6582.t1.CDS4 | 17708370 | 17708621 |
| chr_2 | g6582 | g6582.t1 | exon | g6582.t1.exon5 | 17708683 | 17709095 |
| chr_2 | g6582 | g6582.t1 | cds | g6582.t1.CDS5 | 17708683 | 17709095 |
| chr_2 | g6582 | g6582.t1 | exon | g6582.t1.exon6 | 17709155 | 17709563 |
| chr_2 | g6582 | g6582.t1 | cds | g6582.t1.CDS6 | 17709155 | 17709563 |
| chr_2 | g6582 | g6582.t1 | exon | g6582.t1.exon7 | 17709623 | 17709844 |
| chr_2 | g6582 | g6582.t1 | cds | g6582.t1.CDS7 | 17709623 | 17709844 |
| chr_2 | g6582 | g6582.t1 | TTS | g6582.t1 | 17710445 | 17710445 |
| chr_2 | g6582 | g6582.t1 | TSS | g6582.t1 | NA | NA |
Sequences
>g6582.t1 Gene=g6582 Length=2274
ATGTCATTTTTAAATAATTTGAAAAAAGTTTTCCACTTGGGAAGTGGAGAAACAAAAAAG
AAACGCCTTTATAATAATATTCGAATGGACACAAGTCCCAATGATTTTTGGGACATGATT
GGAGAGCTTGGCGATGGCGCATTTGGTAAAGTTTATAAAGCTCAAAATAAAGAAACAGGA
CAGTATGCTGCTGCAAAAATGTGTACACTTGAGGATGAAGAAAATTTAAGTGATCACATG
GTAGAAATAGACATTTTATCAGAAATAAGACATTCGAATATTGTAGAGCTCTATGAAGCT
TTTTTTATTGATGATAAATTATGGATGTTGATTGAATATTGTGACGGCGGTGCCTTAGAT
AGCATAATGGTAGAATTGGAAAAGCCTTTAACTGAGCCACAAATTGCATATGTAACAAAG
CATATGATAGCTGGACTTAATCATTTGCATAAGAATAAAGTGATCCATAGAGATCTTAAA
GCTGGAAATGTCCTTCTTACAATGGATGGAGGTGTAAAATTAGCGGATTTTGGAGTGTCT
GCGAAAAATAAGCATACAATGCAAAAACATGATACATTTATTGGAACTCCATACTGGATG
GCTCCTGAACTGGTTCTATGTGAGACATTTAGAGATAATCCATATGATTTTAAAGTTGAT
ATTTGGTCGCTGGGCATCACATTAATTGAATTCGCGCAAATGGAACCACCAAATAGTGAA
ATGTCGCCTATGCGCGTTCTATTAAAAATACAAAAAAGTGATCCACCCAAACTTGATCAG
CCATCTAAGTGGTCGAAAGAATTCAACGACTTCCTTGCCAAAGTGCTTATTAAAGATCCA
AATCAAAGACCTGACACAGATGCTCTAATGCAATTGCCATTTATAAATGGGAATCTTGAT
CCTAAACCTGTACGCGATTTATTACTTGAATATAAAGCAGATGTTGTTGAAGAAGAACTT
GTTGATGAAGAGGCTGAGGAGCAGCGAACGCCGGCAATTCAAGACTTGGATGATGATTCT
GCTTCCGTGCAATCACAAGAAACTGACAAACAACCAATATCATCAGAAACAACATTGTCC
CTTTCCAAATCATCCAATACAAGTAAAGAATCAACGCCACAGCCTGATGAGTTTTCCAAA
AAATTAATTCAAGATAGTAGTGGTGGTAATAATAATAATAATGTCAACAATAATCATATC
ATAAATGAATTGCCCGAAAATGAAAAGAAGGTGATTAAACGAGAAAAAGGACCAGCGCCT
TTACCACCACCAATGGTTGCTGCTACTGCAACTACTCCTACTCCAGCAACACCAATATCT
CAAACAGAGGCATCTGATGAGACACCTTTCTCTACAGTCGTTGAGACTCCTGTTACAAAT
CTCAAGATTATTGAGCAAGGTGATGATGATGAACTAAAACACAAAGCATTGAATGAAAAA
GAAGAAATGATTGAATCACCAAGCAAAAACCAAATAAAAGAAATTTTACCTCCACCACCG
CCAGAATTTGCCAATGAAACCACGATTGCAGCCTTTGATGGTTATGAAAAACAACAACAA
CCATTATCGGTCAAAGGATTTGTAAAATCATTTGAAATCGAAAAAGAACAGCCTAAAATA
CGTGAACACGTTGAGCTTAAACGCAATAAATTGAATACATCTACTGTTACAGTAAACCAA
CTACAGGATTCATCAAATAGTCTTGCTAGTAGTAATGGTAATCAAGTTACAATAGTTACA
AGTCATCCTCCTGTTATAATAGATAACAACACAAATACGCTTAATGCAACTACCAGCGAA
TCAAAAACAAAGTTGAAAAAATCTTTGTCGCATGATACAAATACATCTTCGATGAGCAAC
GAAGTTGTAATTTTGTCTAACGAAACAAATAAAACTAAGCATTTAAATGATTCTTTAACA
GATGATGACTATCAATCATATGATAGTCTCGAAAATTCACCGAAATTTAATAATGCAAAT
ATTAACGATGATAATACTTTTATTCAAATCAATACAAGTGCGAATAAACCGAGGCCAAAA
AGTTGGATGAAAGATGAAAAAGACATAAACATTAAAAATTCAATGGAAAATCATTTAGAC
ACCAGTCATGTTTCTGTAGTAACAGTTGGTGATGAGCAAGTAAGAGTAAAAGATTCGAGT
CACTTACTGGTAGATTCAAACAGTGATTTATCGAATGCACATAGTGAGTTAAGTGATGGG
ACAGAATTTACTTCAACTCGTACAAAAACATACGAATGGACAGATTATTTTTAA
>g6582.t1 Gene=g6582 Length=757
MSFLNNLKKVFHLGSGETKKKRLYNNIRMDTSPNDFWDMIGELGDGAFGKVYKAQNKETG
QYAAAKMCTLEDEENLSDHMVEIDILSEIRHSNIVELYEAFFIDDKLWMLIEYCDGGALD
SIMVELEKPLTEPQIAYVTKHMIAGLNHLHKNKVIHRDLKAGNVLLTMDGGVKLADFGVS
AKNKHTMQKHDTFIGTPYWMAPELVLCETFRDNPYDFKVDIWSLGITLIEFAQMEPPNSE
MSPMRVLLKIQKSDPPKLDQPSKWSKEFNDFLAKVLIKDPNQRPDTDALMQLPFINGNLD
PKPVRDLLLEYKADVVEEELVDEEAEEQRTPAIQDLDDDSASVQSQETDKQPISSETTLS
LSKSSNTSKESTPQPDEFSKKLIQDSSGGNNNNNVNNNHIINELPENEKKVIKREKGPAP
LPPPMVAATATTPTPATPISQTEASDETPFSTVVETPVTNLKIIEQGDDDELKHKALNEK
EEMIESPSKNQIKEILPPPPPEFANETTIAAFDGYEKQQQPLSVKGFVKSFEIEKEQPKI
REHVELKRNKLNTSTVTVNQLQDSSNSLASSNGNQVTIVTSHPPVIIDNNTNTLNATTSE
SKTKLKKSLSHDTNTSSMSNEVVILSNETNKTKHLNDSLTDDDYQSYDSLENSPKFNNAN
INDDNTFIQINTSANKPRPKSWMKDEKDINIKNSMENHLDTSHVSVVTVGDEQVRVKDSS
HLLVDSNSDLSNAHSELSDGTEFTSTRTKTYEWTDYF
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g6582.t1 | CDD | cd06611 | STKc_SLK_like | 31 | 310 | 0.0 |
| 5 | g6582.t1 | Gene3D | G3DSA:3.30.200.20 | Phosphorylase Kinase; domain 1 | 17 | 113 | 3.8E-29 |
| 6 | g6582.t1 | Gene3D | G3DSA:1.10.510.10 | Transferase(Phosphotransferase) domain 1 | 114 | 324 | 1.3E-72 |
| 13 | g6582.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 320 | 377 | - |
| 12 | g6582.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 335 | 377 | - |
| 11 | g6582.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 598 | 617 | - |
| 2 | g6582.t1 | PANTHER | PTHR46538 | - | 5 | 699 | 1.9E-156 |
| 3 | g6582.t1 | PANTHER | PTHR46538:SF1 | STE20 LIKE KINASE | 5 | 699 | 1.9E-156 |
| 1 | g6582.t1 | Pfam | PF00069 | Protein kinase domain | 40 | 295 | 1.7E-62 |
| 9 | g6582.t1 | ProSitePatterns | PS00107 | Protein kinases ATP-binding region signature. | 43 | 66 | - |
| 8 | g6582.t1 | ProSitePatterns | PS00108 | Serine/Threonine protein kinases active-site signature. | 154 | 166 | - |
| 14 | g6582.t1 | ProSiteProfiles | PS50011 | Protein kinase domain profile. | 37 | 295 | 48.909 |
| 10 | g6582.t1 | SMART | SM00220 | serkin_6 | 37 | 295 | 2.1E-84 |
| 4 | g6582.t1 | SUPERFAMILY | SSF56112 | Protein kinase-like (PK-like) | 33 | 301 | 2.16E-87 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0005524 | ATP binding | MF |
| GO:0004672 | protein kinase activity | MF |
| GO:0006468 | protein phosphorylation | BP |
KEGG
Orthology
- This transcript has been annotated with K08836 (STE20-like kinase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
