General information for g6720.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Palmitoleoyl-protein carboxylesterase NOTUM.
Parent gene
- The parent gene g6720 is located on chr_2: 18300472..18302504 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6720 | g6720.t1 | TSS | g6720.t1 | 18294000 | 18294000 |
| chr_2 | g6720 | g6720.t1 | isoform | g6720.t1 | 18294427 | 18302504 |
| chr_2 | g6720 | g6720.t1 | exon | g6720.t1.exon1 | 18294427 | 18294450 |
| chr_2 | g6720 | g6720.t1 | cds | g6720.t1.CDS1 | 18294427 | 18294450 |
| chr_2 | g6720 | g6720.t1 | exon | g6720.t1.exon2 | 18300294 | 18300408 |
| chr_2 | g6720 | g6720.t1 | cds | g6720.t1.CDS2 | 18300294 | 18300408 |
| chr_2 | g6720 | g6720.t1 | exon | g6720.t1.exon3 | 18300472 | 18300727 |
| chr_2 | g6720 | g6720.t1 | cds | g6720.t1.CDS3 | 18300472 | 18300727 |
| chr_2 | g6720 | g6720.t1 | exon | g6720.t1.exon4 | 18300793 | 18301130 |
| chr_2 | g6720 | g6720.t1 | cds | g6720.t1.CDS4 | 18300793 | 18301130 |
| chr_2 | g6720 | g6720.t1 | exon | g6720.t1.exon5 | 18301188 | 18301391 |
| chr_2 | g6720 | g6720.t1 | cds | g6720.t1.CDS5 | 18301188 | 18301391 |
| chr_2 | g6720 | g6720.t1 | exon | g6720.t1.exon6 | 18301462 | 18302504 |
| chr_2 | g6720 | g6720.t1 | cds | g6720.t1.CDS6 | 18301462 | 18302504 |
| chr_2 | g6720 | g6720.t1 | TTS | g6720.t1 | NA | NA |
Sequences
>g6720.t1 Gene=g6720 Length=1980
ATGAAGAGTTTTAAAATAATTTTGATTATATTTGCATTAACACTTCACTACATTCCATGT
GATTGTCGATCATTTTCAACAAATTTCACTAGCGTCAATAATGTTAACAATACATCAAGA
ACGGCACGACAAAATAAAGGAGGCGATTTTGAAAGTAACTCCATCCAGTGGATAGTAGGA
AAAACTGAAATGAACTCTTCAAATGGATACTCAAACAAGTCTACAAAACTCAAGAGAGTG
TTTCTCTCCAATCGAGACATCACCTGCAATGATGGATCGCAAGCCGGATTCTATTTGCGC
AAATCACCAGGATCACGCCGTTGGGTAGTATTCTTTGAAGGCGGATGGCATTGCTACGAT
CATAAATCATGTAAAATGCGTTGGACAAAAATGAGGCATTTGATGACATCAACTACATGG
CCTGAAACGAGAGATGTCGGTGGTATTTTATCACCATTACAATCTGAAAATCCATATTGG
TTCAATGCAAATCATGTTTTTGTACCTTACTGTAGTAGTGATTCATGGAGTGGAAATCGA
TCAAAACCTGAAACACGTGACAGTTTTAGATTTATGGGTTCTTTGATAGTTCGACAAGTC
ATTGCCGATTTGATTCCCCTTGGTTTGGGCAATGCTCAGGGTACAGATCTTTTAATGGCT
GGTTCAAGCGCTGGTGGTTTGGGTGTTATGTTAAATATGGATAAAATCAGAGCATTTCTT
CAAGATGAAAGAGGTCTCAATGTACATGTTCGAGGTGTCAGTGATTCAGGATGGTTTTTA
GACAGAGAACCATTTTTACCTGGTACAATCGCTGGAGCAGAAGCTGTACGTCAAGGTTGG
AAATTGTGGAACGGTGTTTTACCAGAAGATTGTGTAGCTGAACAAAAAGAACCATGGAGA
TGTTATTTCGGTCATCGACTTTACAATACTTTGAAATCGCCTCTTTTTATTTTTCAATGG
CTTTTCGATGAGGCACAAATGAGAGCTGATAATGTTGGTGCTCCAGTTACACCACAACAA
TGGGATTATATTCATGAAATGGGTGGTGCATTAAGAAAATCACTTGAAAATGTCACTGCT
GTTTTTGCACCTTCTTGCATCGGACATTCAGTGCTCACAAAACGTGATTGGCAAAATATT
AAAATTGACGATGTCTCATTAACTGATTCATTAAGATGTTGGGAAAATTCACTTTTGAAA
GATAGAAAAATAACATTGAAATTAAGTGAGAATAAGAGACTTAATAAACATAAAAAACAC
AAGGGTAGAAATCATCAAAGTGATCAACCACATCTTACAAATGAAGAAAGAGAGCAAAGA
AGAAGAGAACGACGACGCGAGCAACGAATGAAACTTACACCAGAGGAGCGTGAACAAAGA
CGATTAGAACGACGTGAAAGACGAAGACAAGAAAGACTTCTTCAAAAAATGGAAGCCAAA
AATCAACCACCACGCGCCCAGCGCTCACCTCAGGAAAATGAAATGAATGACAAAAATTCA
TTACCAAAGATAATTGAAAATGAGCCAGTTGCAAAGCAGCAACAAAAAAAACGTCGTCAA
CAATTTAATCAAAGACGACGTATGCAACAAATTGAATCACAAAATGCAACACGTTTGCCA
AAACATCGTAGACAGAATCATAAGCAACATAATTTAAATACAAAAGGACCAAACAAGAGA
CGTCATGGCGAACATAATCGCCAGAAACTTCTCAAAGCACAAAATCAATTAATTGAAATT
AAAGAACCGAAGAAATGTGGAATGAAGTTATTAGAACGATGTAGTTGGCCTCAATGTAAT
CATTCTTGTCCATCACTAACTAATCCACTTACTGGGGAGGAAATGAAATTTCTTGAATTA
TTAACATCATTTGGACTAGATATTGACGCTGTTGCACAAGCACTCGGTGTTGATTTGCAT
ACACTTAACAATATGGACCATAAAGAGTTAATTAAATTGTTTACTGAATCCACAAGTTAG
>g6720.t1 Gene=g6720 Length=659
MKSFKIILIIFALTLHYIPCDCRSFSTNFTSVNNVNNTSRTARQNKGGDFESNSIQWIVG
KTEMNSSNGYSNKSTKLKRVFLSNRDITCNDGSQAGFYLRKSPGSRRWVVFFEGGWHCYD
HKSCKMRWTKMRHLMTSTTWPETRDVGGILSPLQSENPYWFNANHVFVPYCSSDSWSGNR
SKPETRDSFRFMGSLIVRQVIADLIPLGLGNAQGTDLLMAGSSAGGLGVMLNMDKIRAFL
QDERGLNVHVRGVSDSGWFLDREPFLPGTIAGAEAVRQGWKLWNGVLPEDCVAEQKEPWR
CYFGHRLYNTLKSPLFIFQWLFDEAQMRADNVGAPVTPQQWDYIHEMGGALRKSLENVTA
VFAPSCIGHSVLTKRDWQNIKIDDVSLTDSLRCWENSLLKDRKITLKLSENKRLNKHKKH
KGRNHQSDQPHLTNEEREQRRRERRREQRMKLTPEEREQRRLERRERRRQERLLQKMEAK
NQPPRAQRSPQENEMNDKNSLPKIIENEPVAKQQQKKRRQQFNQRRRMQQIESQNATRLP
KHRRQNHKQHNLNTKGPNKRRHGEHNRQKLLKAQNQLIEIKEPKKCGMKLLERCSWPQCN
HSCPSLTNPLTGEEMKFLELLTSFGLDIDAVAQALGVDLHTLNNMDHKELIKLFTESTS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 9 | g6720.t1 | Coils | Coil | Coil | 460 | 480 | - |
| 7 | g6720.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 410 | 566 | - |
| 4 | g6720.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 426 | 478 | - |
| 6 | g6720.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 482 | 500 | - |
| 5 | g6720.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 540 | 566 | - |
| 2 | g6720.t1 | PANTHER | PTHR21562:SF45 | PALMITOLEOYL-PROTEIN CARBOXYLESTERASE NOTUM | 36 | 624 | 5.7E-182 |
| 3 | g6720.t1 | PANTHER | PTHR21562 | NOTUM-RELATED | 36 | 624 | 5.7E-182 |
| 1 | g6720.t1 | Pfam | PF03283 | Pectinacetylesterase | 78 | 394 | 5.1E-69 |
| 11 | g6720.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 12 | g6720.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 13 | g6720.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 15 | - |
| 14 | g6720.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 16 | 20 | - |
| 10 | g6720.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 659 | - |
| 8 | g6720.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 22 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K19882 (O-palmitoleoyl-L-serine hydrolase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
