General information for g674.t40
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Glucose-6-phosphatase.
Parent gene
- The parent gene g674 is located on chr_3: 5210514..5211200 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g674 | g674.t40 | TTS | g674.t40 | 5209165 | 5209165 |
| chr_3 | g674 | g674.t40 | isoform | g674.t40 | 5209193 | 5211200 |
| chr_3 | g674 | g674.t40 | exon | g674.t40.exon1 | 5209193 | 5209258 |
| chr_3 | g674 | g674.t40 | cds | g674.t40.CDS1 | 5209256 | 5209258 |
| chr_3 | g674 | g674.t40 | exon | g674.t40.exon2 | 5210032 | 5210239 |
| chr_3 | g674 | g674.t40 | cds | g674.t40.CDS2 | 5210032 | 5210239 |
| chr_3 | g674 | g674.t40 | exon | g674.t40.exon3 | 5210295 | 5211200 |
| chr_3 | g674 | g674.t40 | cds | g674.t40.CDS3 | 5210295 | 5211109 |
| chr_3 | g674 | g674.t40 | TSS | g674.t40 | 5211298 | 5211298 |
Sequences
>g674.t40 Gene=g674 Length=1180
ATGGAGATAATTGACAGCTTATGGAAAATAGAAATCGATTTAATTATTAAAATTCAAAAC
AAGTAAGTTTTCCAAAGAATTTCATAAATTTATGAAAAATTTCCATGAAAAAAATTTCCC
TTTCAGGTTTTTCAAATATGAAGAATTTCTCGGATATTTTTCAACATTTTTTGGTTCGGG
ATATCTCCACTTGTACATTATTCCATTGTTTGCAATGTACAACACAAAAGTTTATCATCG
TTTATTGATTTCATGCATTGCGACTGATTTTATTAATCTCATTATAAAATGGATTTTAGA
TGAAGATCGACCTTACTGGTGGGTCAATGAAACAAAAGCTTACACATCACTCACCCGACC
AATATTATATCAAACTGAAAGAACTTGTGAGACATCGGGTGGATCACCATCAGGTCATTC
GATGATTGCTGCATGTTTTCTATACATCATCTATGACGAAATCAACCGACAAATCGACAA
TCGTCCTTCAATGTCGCACAAAGTCATACTGAAAATTCTAAACAAAATTGGACTGACTTT
TATACTTACAATTACTGCCATATCGAGAATGTACTTTGCTGCACATTTTTTGCATCAATG
TATTTTAGGTTTCATGCTTGGACTTTTAGTAGCAAATTTAATGGCAAATCATGGATTGGC
TGAAAAATTTTCGAGTTTTGAAAAACGAACTTGGATGACAATAATTGCTTGGATGATAAT
TTTGGTAGTGTCAATTTATTGGGCCCATAAATTAATTAGTGGAAATCCAATGAAAACAGT
AGAATTGGCATTCAAACATTGCAAAGATCCACTCTATCCTAAGCCTGAAACTACTGTCGT
TTTTTCTGCTCTCCGATGCATTGCGATGTCTTGTGGAATTTTGTTAAATGCACCAATTTA
CAAAAGAGAGTCAAAATTGAAATTCTTTGAATCAGCAGTAATGACAATATGTTTAATTAT
TCTTCAATTCTGTGCAATTTATGAAACACCAAAGAATTACAAAATTATCATTTTCTATAG
TTATTCATTTATTATTTATGCATTTTTCCAATTTATGTTCATCTATTTTGTTCCAAAAAT
TGCAACAATAACTAATGAAAATTCTCAAGAAAAATAAAAATAATTAAATGTTTAACGTAT
TTATAGAAAAATTCAAATAAAACCATATAAATGTTAAAAA
>g674.t40 Gene=g674 Length=341
MKNFHEKNFPFRFFKYEEFLGYFSTFFGSGYLHLYIIPLFAMYNTKVYHRLLISCIATDF
INLIIKWILDEDRPYWWVNETKAYTSLTRPILYQTERTCETSGGSPSGHSMIAACFLYII
YDEINRQIDNRPSMSHKVILKILNKIGLTFILTITAISRMYFAAHFLHQCILGFMLGLLV
ANLMANHGLAEKFSSFEKRTWMTIIAWMIILVVSIYWAHKLISGNPMKTVELAFKHCKDP
LYPKPETTVVFSALRCIAMSCGILLNAPIYKRESKLKFFESAVMTICLIILQFCAIYETP
KNYKIIIFYSYSFIIYAFFQFMFIYFVPKIATITNENSQEK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 5 | g674.t40 | Gene3D | G3DSA:1.20.144.10 | - | 13 | 200 | 8.8E-14 |
| 2 | g674.t40 | PANTHER | PTHR12591 | GLUCOSE-6-PHOSPHATASE | 12 | 338 | 1.7E-63 |
| 3 | g674.t40 | PANTHER | PTHR12591:SF0 | FI19814P1 | 12 | 338 | 1.7E-63 |
| 25 | g674.t40 | PIRSF | PIRSF000905 | G6Pase | 6 | 341 | 1.1E-48 |
| 1 | g674.t40 | Pfam | PF01569 | PAP2 superfamily | 51 | 185 | 1.5E-20 |
| 13 | g674.t40 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 19 | - |
| 20 | g674.t40 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 20 | 43 | - |
| 10 | g674.t40 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 44 | 49 | - |
| 18 | g674.t40 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 50 | 69 | - |
| 14 | g674.t40 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 70 | 102 | - |
| 21 | g674.t40 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 103 | 121 | - |
| 6 | g674.t40 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 122 | 141 | - |
| 16 | g674.t40 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 142 | 160 | - |
| 12 | g674.t40 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 161 | 165 | - |
| 17 | g674.t40 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 166 | 189 | - |
| 9 | g674.t40 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 190 | 200 | - |
| 19 | g674.t40 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 201 | 219 | - |
| 11 | g674.t40 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 220 | 248 | - |
| 24 | g674.t40 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 249 | 270 | - |
| 8 | g674.t40 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 271 | 281 | - |
| 23 | g674.t40 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 282 | 300 | - |
| 15 | g674.t40 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 301 | 305 | - |
| 22 | g674.t40 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 306 | 327 | - |
| 7 | g674.t40 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 328 | 341 | - |
| 32 | g674.t40 | SMART | SM00014 | acid_phosph_2 | 46 | 185 | 2.6E-15 |
| 4 | g674.t40 | SUPERFAMILY | SSF48317 | Acid phosphatase/Vanadium-dependent haloperoxidase | 22 | 184 | 2.09E-14 |
| 26 | g674.t40 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 20 | 42 | - |
| 29 | g674.t40 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 47 | 69 | - |
| 31 | g674.t40 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 163 | 185 | - |
| 28 | g674.t40 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 200 | 219 | - |
| 27 | g674.t40 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 278 | 300 | - |
| 30 | g674.t40 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 305 | 327 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004346 | glucose-6-phosphatase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01084 (glucose-6-phosphatase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed