General information for g6912.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein 5NUC.
Parent gene
- The parent gene g6912 is located on chr_2: 19897594..19902814 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6912 | g6912.t1 | isoform | g6912.t1 | 19894197 | 19897594 |
| chr_2 | g6912 | g6912.t1 | exon | g6912.t1.exon1 | 19894197 | 19894492 |
| chr_2 | g6912 | g6912.t1 | cds | g6912.t1.CDS1 | 19894197 | 19894492 |
| chr_2 | g6912 | g6912.t1 | exon | g6912.t1.exon2 | 19894554 | 19894635 |
| chr_2 | g6912 | g6912.t1 | cds | g6912.t1.CDS2 | 19894554 | 19894635 |
| chr_2 | g6912 | g6912.t1 | exon | g6912.t1.exon3 | 19894736 | 19894989 |
| chr_2 | g6912 | g6912.t1 | cds | g6912.t1.CDS3 | 19894736 | 19894989 |
| chr_2 | g6912 | g6912.t1 | exon | g6912.t1.exon4 | 19895052 | 19895179 |
| chr_2 | g6912 | g6912.t1 | cds | g6912.t1.CDS4 | 19895052 | 19895179 |
| chr_2 | g6912 | g6912.t1 | exon | g6912.t1.exon5 | 19895238 | 19897594 |
| chr_2 | g6912 | g6912.t1 | cds | g6912.t1.CDS5 | 19895238 | 19897594 |
| chr_2 | g6912 | g6912.t1 | TTS | g6912.t1 | 19897725 | 19897725 |
| chr_2 | g6912 | g6912.t1 | TSS | g6912.t1 | NA | NA |
Sequences
>g6912.t1 Gene=g6912 Length=3117
ATGTTTTTTTCACATTTGACGACAATTGTTCTGCTACTGCTAGCTGTACACACCTTCTCA
GCACCGCAAAAGAAATCAACAGATGAGAACTTTAAATTAATTATTTTACACAACAATGAT
ATGCATGCACGTTTTGAGCAGACAAACGCAGCGAGCGCAAGATGTACAGACGAAGATCGA
GAGAAAAACAATTGCTATGGAGGTTTTGCGAGAGTTGCGCACGTTGTGAGAGACTATCGA
AAGAGAGCAAATACTGGCGAGATTCCAAGAGTTCTCTATTTGAATGCTGGTGATACGTAT
ACTGGAACACCTTGGTTTGCATTATTCAAAGATAATATTACGTCTGCTTTTCTTGAATTA
CTGAAACCTGATGCAATTTCACTCGGCAATCACGAGTTTGATGAAGGGACTGAGAATCTT
GCAAAATTTCTTAAAGAAATTGATTTTCCAGTATTAGCAGCAAACTTAGATTTAGAAAAG
GAGCCGTCATTGAAAGTTGACATTCTGAAACCATCACATATTTTCGAGATCGATGGCATC
AAAATTGGTGTAGTAGGTTACTTGACACCTGAAACTAAAGAAGTTGCAAAGGGCAATCAA
GTAGAATTTATTGACGAAATTGAAGCTATTAATAAGGAGGCCGCACGTCTGAAAGAAGAA
GGTATTAAAATTATAATTGCACTTGGTCATTCTGGAATTGAGCGTGATAAGCAAATTGCT
ATGGAATGTCCGGACATTGATTTGATTATTGGTGGACATTCTCATACATTTTTATACAAT
GGAACCAAGCCCGATATTGATGAACCTTATGATACTTATCCAATAATGATAGTTCAACGT
AATGGAAAGAAAGTGCCTGTTGTTCAAAGTTATGCTTATACAAAATATCTTGGTTACATT
CATCTTGAATTTGATGGTGAAGGAAATATGGTTGAGATTGATGGAAATCCAATTCTTCTT
AATCATGACATTCCACGTGAACAAGACGTTTTAGATTTACTTCAAAAGTATCGACCAGCA
GTATTAGCATTACAAACAGAAGTAGTTGGTCAAACTCGAGTCAATTTAGAAGGTTCATGT
CGTCGTGTTGAATGCAATTTGGGAAATTTCATTGCTGATTCAATGGTAGATTGGAATGCA
TTGAGATATGATGGTATAGGTTGGACAGATGCAGCAATTGGTATCATACAAGGTGGTGGA
GTAAGAGCTTCAATCACAAGAACAGTCAGTGGAAATATCACAATGGAAGATATTGCTACA
GTTTTACCATTTGACAGTAAAGTTGTAGTTATTGAAATCACTGGTGAGACATTGTTAAAG
GCTTTTGAACATTCAGTTGCACGTTATACAGAAGGTGAAGGGCGTGGAGAATTTTTGCAA
TATTCTGGAGTGCAAATTGAATACGATATGAATCGAGAATCAGGCAACCGAGTTGTTAAT
GTAAAAGTTCTTTGCGCTTATTGTGAAGTTCCACAACTTGAAGATCTTGTTTTGCATAAA
ACATATCGCATAGTTATGCAAGATTTTCTAGCTAACGGTGGCGATGGGTATGAAATGTTT
AAAAATTCACAAGTTGGAATAGAAACTGAAACAGTTGATGTTGATGTTTTAAAGAGCTAT
ATGCTTAAAAAATCACCAATTTATCCAGCTGTTGAATGGAGAATCACAATTAAAGATTTA
GTTGATCCAAATGATGAGATTGTTGGAAGCAGCCGAGTATTTTTAGATGGAAATTGTTAC
ACAACTGAATGCAACTTGGGTAACTTTATCACTGATGCATTTGTCGATTGGTATGCAATG
AATTCTAATGTTCAAACGGGTTGGACTGATGCATCGATTGCTCTCATTCCTGCAAGAAAT
ATTCAATTTTCAATTGATCCAAAAGAAACTAAAGGTGATATTACAAAAGCATTAGCAAAC
AAAATTCTTCCATTAAACAATAAACTTGTTGTTGTTGAAATCTCTGGTGCAGTTTTTATG
CAAGCACTTGAGCATGCTGTCAGCAGTTATTACGATGGTGCTCAAACCGAGTTCTTGCAA
ATGTCTGGAGTACAAGTTGAATATGATATCAATAATGAAGTAGGAAGTCGAGTTGTTTCT
GCTAAAATCCTTTGCTCTTCATGTACACTTCCAGAACTTGAAACAATTAATACTTCTGCA
AATTATAAAATTTTAATGCCACAAAATCTTGCAAACGGTGATGAAGGTTACACGATGTTC
TCAAACCATATCAGCAGTATTTTAGAAGTTACCGAACTTGATGCTTTCTTGAATTATTTG
AAGAAAAAGTCACCGATTTATCCAGCGATTGAATGGCGTATTACAATTAAAAACTTTGTT
GATCCATCTGTGGAAGTTGTTGGTAGCACACGCGTTTTCTTAAATAATGATTGTAGTCTT
GGTGAATGTAATTTGGGTAACTTCATTACTGATGCAATGGTTGATTGGTATTCAAATAAG
TACAACAAAGACGAATATTGGACTGATGCATCAATTGCTGTTATTCAAGGCAGTAGAATC
AAAGCGTCAATTGATGCAAAAGCAAATAATGGAAGTATTTTCAAGAGTGATGTTGAGAAA
ATTTTCAGTCCAATTCCATTTACACTTCAGTTAGTAACACTCAAAGGAAGTGAATTATTA
GACATGTTAGAATATTCAATTTCATCATATACTGATGAAGGTATTATTGACACACAATTC
TTACAATTATCAGGCATTCAAGTTGTTTATGACACAAGAAAAGCATCTGGAAGTCGTATT
ACAGACATTAAAGTTCTTTGCGCTCAATGTAGTGTTCCTGAACTTGTGAATGTTGATAGA
AATCTTGATTATAAAATAATTATGCAATCCGCAATGTTAACTGAAGACTATTATAATGTC
ATATCAAGTAATAAAATAGAGGAACTTGATGAGAGTGATATGAATGCCTTCTTAAATTAT
TTAAAGAAAAAGTCTCCAATTTATCCAGCAGTTGAGTGGAGAATAACGATTTTAGGTGAT
GAAGTTACAACTACAACCGAAGCTACAAAAACAACGACAGGAGGCAGTTCAAATTTGCAG
CTTTCACTTGCATTATTGCTTATATCAATATTGATGAGTTTCCTTAACAAGAGCTAG
>g6912.t1 Gene=g6912 Length=1038
MFFSHLTTIVLLLLAVHTFSAPQKKSTDENFKLIILHNNDMHARFEQTNAASARCTDEDR
EKNNCYGGFARVAHVVRDYRKRANTGEIPRVLYLNAGDTYTGTPWFALFKDNITSAFLEL
LKPDAISLGNHEFDEGTENLAKFLKEIDFPVLAANLDLEKEPSLKVDILKPSHIFEIDGI
KIGVVGYLTPETKEVAKGNQVEFIDEIEAINKEAARLKEEGIKIIIALGHSGIERDKQIA
MECPDIDLIIGGHSHTFLYNGTKPDIDEPYDTYPIMIVQRNGKKVPVVQSYAYTKYLGYI
HLEFDGEGNMVEIDGNPILLNHDIPREQDVLDLLQKYRPAVLALQTEVVGQTRVNLEGSC
RRVECNLGNFIADSMVDWNALRYDGIGWTDAAIGIIQGGGVRASITRTVSGNITMEDIAT
VLPFDSKVVVIEITGETLLKAFEHSVARYTEGEGRGEFLQYSGVQIEYDMNRESGNRVVN
VKVLCAYCEVPQLEDLVLHKTYRIVMQDFLANGGDGYEMFKNSQVGIETETVDVDVLKSY
MLKKSPIYPAVEWRITIKDLVDPNDEIVGSSRVFLDGNCYTTECNLGNFITDAFVDWYAM
NSNVQTGWTDASIALIPARNIQFSIDPKETKGDITKALANKILPLNNKLVVVEISGAVFM
QALEHAVSSYYDGAQTEFLQMSGVQVEYDINNEVGSRVVSAKILCSSCTLPELETINTSA
NYKILMPQNLANGDEGYTMFSNHISSILEVTELDAFLNYLKKKSPIYPAIEWRITIKNFV
DPSVEVVGSTRVFLNNDCSLGECNLGNFITDAMVDWYSNKYNKDEYWTDASIAVIQGSRI
KASIDAKANNGSIFKSDVEKIFSPIPFTLQLVTLKGSELLDMLEYSISSYTDEGIIDTQF
LQLSGIQVVYDTRKASGSRITDIKVLCAQCSVPELVNVDRNLDYKIIMQSAMLTEDYYNV
ISSNKIEELDESDMNAFLNYLKKKSPIYPAVEWRITILGDEVTTTTEATKTTTGGSSNLQ
LSLALLLISILMSFLNKS
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 35 | g6912.t1 | CDD | cd07409 | MPP_CD73_N | 33 | 320 | 5.06791E-143 |
| 27 | g6912.t1 | Coils | Coil | Coil | 200 | 220 | - |
| 23 | g6912.t1 | Gene3D | G3DSA:3.60.21.10 | - | 22 | 351 | 1.8E-98 |
| 26 | g6912.t1 | Gene3D | G3DSA:3.90.780.10 | - | 352 | 558 | 3.1E-70 |
| 25 | g6912.t1 | Gene3D | G3DSA:3.90.780.10 | - | 566 | 777 | 6.2E-52 |
| 24 | g6912.t1 | Gene3D | G3DSA:3.90.780.10 | - | 785 | 998 | 7.7E-44 |
| 6 | g6912.t1 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 22 | 559 | 4.4E-293 |
| 9 | g6912.t1 | PANTHER | PTHR11575:SF25 | 5’-NUCLEOTIDASE | 22 | 559 | 4.4E-293 |
| 7 | g6912.t1 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 564 | 777 | 4.4E-293 |
| 10 | g6912.t1 | PANTHER | PTHR11575:SF25 | 5’-NUCLEOTIDASE | 564 | 777 | 4.4E-293 |
| 5 | g6912.t1 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 783 | 1014 | 4.4E-293 |
| 8 | g6912.t1 | PANTHER | PTHR11575:SF25 | 5’-NUCLEOTIDASE | 783 | 1014 | 4.4E-293 |
| 15 | g6912.t1 | PRINTS | PR01607 | Apyrase family signature | 31 | 49 | 2.4E-37 |
| 16 | g6912.t1 | PRINTS | PR01607 | Apyrase family signature | 220 | 237 | 2.4E-37 |
| 12 | g6912.t1 | PRINTS | PR01607 | Apyrase family signature | 239 | 262 | 2.4E-37 |
| 14 | g6912.t1 | PRINTS | PR01607 | Apyrase family signature | 285 | 305 | 2.4E-37 |
| 13 | g6912.t1 | PRINTS | PR01607 | Apyrase family signature | 412 | 435 | 2.4E-37 |
| 11 | g6912.t1 | PRINTS | PR01607 | Apyrase family signature | 495 | 514 | 2.4E-37 |
| 4 | g6912.t1 | Pfam | PF00149 | Calcineurin-like phosphoesterase | 35 | 256 | 6.6E-12 |
| 3 | g6912.t1 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 348 | 521 | 6.3E-41 |
| 1 | g6912.t1 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 568 | 741 | 1.7E-29 |
| 2 | g6912.t1 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 786 | 950 | 6.7E-22 |
| 30 | g6912.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 31 | g6912.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 5 | - |
| 32 | g6912.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 6 | 16 | - |
| 34 | g6912.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 17 | 20 | - |
| 29 | g6912.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 1018 | - |
| 33 | g6912.t1 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 1019 | 1036 | - |
| 28 | g6912.t1 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 1037 | 1038 | - |
| 37 | g6912.t1 | ProSitePatterns | PS00786 | 5’-nucleotidase signature 2. | 123 | 134 | - |
| 20 | g6912.t1 | SUPERFAMILY | SSF56300 | Metallo-dependent phosphatases | 31 | 341 | 6.75E-87 |
| 17 | g6912.t1 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 347 | 559 | 1.96E-55 |
| 19 | g6912.t1 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 565 | 778 | 1.96E-41 |
| 18 | g6912.t1 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 785 | 999 | 3.4E-35 |
| 22 | g6912.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 36 | g6912.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 21 | g6912.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 20 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0046872 | metal ion binding | MF |
| GO:0016787 | hydrolase activity | MF |
| GO:0000166 | nucleotide binding | MF |
| GO:0016788 | hydrolase activity, acting on ester bonds | MF |
| GO:0009166 | nucleotide catabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K19970 (5’-nucleotidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
