Gene loci information

Transcript annotation

  • This transcript has been annotated as Molybdenum cofactor biosynthesis protein 1.

Parent gene

Gene structure

  • The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.

Chromosome Gene Transcript Category ID Start End
chr_2 g6934 g6934.t2 isoform g6934.t2 20040689 20041967
chr_2 g6934 g6934.t2 exon g6934.t2.exon1 20040689 20040850
chr_2 g6934 g6934.t2 cds g6934.t2.CDS1 20040689 20040850
chr_2 g6934 g6934.t2 exon g6934.t2.exon2 20040908 20041844
chr_2 g6934 g6934.t2 cds g6934.t2.CDS2 20040908 20041844
chr_2 g6934 g6934.t2 exon g6934.t2.exon3 20041912 20041967
chr_2 g6934 g6934.t2 cds g6934.t2.CDS3 20041912 20041967
chr_2 g6934 g6934.t2 TSS g6934.t2 NA NA
chr_2 g6934 g6934.t2 TTS g6934.t2 NA NA

Sequences

>g6934.t2 Gene=g6934 Length=1155
ATGTATCTAAGAAAGATTTATTCAAAGAATGTCATTGAATGCTTAAATTCATCTTCATAT
ATCAATCATCTGCGAAATTTGTCATCAAAATCATTAAAACCAAATGTTGAATTTATCACT
GATAATGAAAAAATTACAACGCAAATCGAACAACGTAGAAATAAGCAACAAGTTTCACCA
TTAATCGATTCTTTTGGAAGATTTCATTCATATTTGAGAATATCATTAACTGAAAAGTGC
AATCTGAGATGTCAATATTGCATGCCTGAAGAAGGTGTGCCATTGTCTCCCAAATCACAT
CTTTTGACAACTGATGAAATTATTTACATATCAAAACTATTTATTGATCAAGGTGTAAGA
AAAATTCGTTTAACAGGAGGTGAGCCCACCATAAGAAAGGACTTGGTTGAAATCATAGCA
AGACTTAAAGAAATTCCACTACTTGAGAATATTAGCATTACTACAAATGGACTAGTGTTA
ACAAGAAATCTTGTCAGTTATCAACGTGCTGGACTTGATTCACTTAATATTTCTCTTGAT
ACTCTTCAGCCTAAAAAATATGAAACAATAACAAGGAGAAAAGGTTTCGAGAGAGTTGTA
GCTGGAATAGATTTAGCAATTCAATTGGGTTACAAACCAAAAATAAATTGTGTAGCTATG
AAGAATTTCAACGAAGATGAAATTTGTGACTTTGTTGAATGGACTAAAGATCGAAATGTT
GATATTAGATTTATAGAGTACATGCCTTTTTCTGGAAATAGGTGGGAGACTGAAAAACTT
TTATCATTCCGTGACATGTTGAATTTAATTCAACAAAAATATCCAACATTTGAAGCCCTT
GAGAACAAACCAAATGATACTTCAAAAGCTTTTAGAGTTCCTGGCTATGAAGGTCAAGTT
GGTTTCATTACTTCAATGACAGAACATTTCTGTGGTTCATGTAACCGCTTGAGAATTACT
GCAGATGGAAATTTAAAAGTTTGTTTATTCGGAAACAAAGAGGTATCATTAAGAGATGCA
ATTCGTAATGGATGCTCTGAAGATGATTTGATTGCATTGATTGGTGCATCAGTTCTAAGA
AAAAAGAAACAACATGCAGGCATGTTAAATCTAAAATCTATGGAAAATCGTCCAATGATT
TTAATTGGAGGATAA

>g6934.t2 Gene=g6934 Length=384
MYLRKIYSKNVIECLNSSSYINHLRNLSSKSLKPNVEFITDNEKITTQIEQRRNKQQVSP
LIDSFGRFHSYLRISLTEKCNLRCQYCMPEEGVPLSPKSHLLTTDEIIYISKLFIDQGVR
KIRLTGGEPTIRKDLVEIIARLKEIPLLENISITTNGLVLTRNLVSYQRAGLDSLNISLD
TLQPKKYETITRRKGFERVVAGIDLAIQLGYKPKINCVAMKNFNEDEICDFVEWTKDRNV
DIRFIEYMPFSGNRWETEKLLSFRDMLNLIQQKYPTFEALENKPNDTSKAFRVPGYEGQV
GFITSMTEHFCGSCNRLRITADGNLKVCLFGNKEVSLRDAIRNGCSEDDLIALIGASVLR
KKKQHAGMLNLKSMENRPMILIGG

Protein features from InterProScan

Transcript Database ID Name Start End E.value
9 g6934.t2 CDD cd01335 Radical_SAM 74 271 5.10307E-21
8 g6934.t2 Gene3D G3DSA:3.20.20.70 Aldolase class I 58 384 7.6E-110
6 g6934.t2 Hamap MF_01225_B GTP 3’,8-cyclase [moaA]. 61 384 37.410011
4 g6934.t2 PANTHER PTHR22960:SF26 MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1 61 373 4.9E-150
5 g6934.t2 PANTHER PTHR22960 MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A 61 373 4.9E-150
1 g6934.t2 Pfam PF04055 Radical SAM superfamily 74 234 5.2E-28
2 g6934.t2 Pfam PF13353 4Fe-4S single cluster domain 76 181 1.4E-7
3 g6934.t2 Pfam PF06463 Molybdenum Cofactor Synthesis C 240 366 6.0E-39
10 g6934.t2 ProSitePatterns PS01305 moaA / nifB / pqqE family signature. 76 87 -
13 g6934.t2 SFLD SFLDG01383 cyclic pyranopterin phosphate synthase (MoaA-like) 59 384 0.0
14 g6934.t2 SFLD SFLDG01216 thioether bond formation requiring one auxiliary iron-sulfur cluster 59 384 0.0
11 g6934.t2 SMART SM00729 MiaB 70 272 2.1E-11
7 g6934.t2 SUPERFAMILY SSF102114 Radical SAM enzymes 60 335 1.44E-76
12 g6934.t2 TIGRFAM TIGR02666 moaA: molybdenum cofactor biosynthesis protein A 61 384 2.3E-110

Transmembrane regions from TMHMM

Disordered region

IUPRED3 score over 0.5 is predictive of a disordered region.

GO terms from InterProScan

GOID TERM ONTOLOGY
GO:0006777 Mo-molybdopterin cofactor biosynthetic process BP
GO:0046872 metal ion binding MF
GO:0051539 4 iron, 4 sulfur cluster binding MF
GO:0003824 catalytic activity MF
GO:0051536 iron-sulfur cluster binding MF
GO:0019008 molybdopterin synthase complex CC

KEGG

Orthology

Pathway

  • This transcript belongs to the following pathways

Expression

Transcript expression in Pv11 cells

TPM values are indicated as average +/- STDEV.

Differential expression

Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.

Raw TPM values