General information for g6936.t33
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Triosephosphate isomerase.
Parent gene
- The parent gene g6936 is located on chr_2: 20044463..20045782 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g6936 | g6936.t33 | isoform | g6936.t33 | 20044082 | 20045161 |
| chr_2 | g6936 | g6936.t33 | exon | g6936.t33.exon1 | 20044082 | 20044092 |
| chr_2 | g6936 | g6936.t33 | exon | g6936.t33.exon2 | 20044275 | 20044376 |
| chr_2 | g6936 | g6936.t33 | cds | g6936.t33.CDS1 | 20044302 | 20044376 |
| chr_2 | g6936 | g6936.t33 | exon | g6936.t33.exon3 | 20044463 | 20044540 |
| chr_2 | g6936 | g6936.t33 | cds | g6936.t33.CDS2 | 20044463 | 20044540 |
| chr_2 | g6936 | g6936.t33 | exon | g6936.t33.exon4 | 20044607 | 20045161 |
| chr_2 | g6936 | g6936.t33 | cds | g6936.t33.CDS3 | 20044607 | 20045161 |
| chr_2 | g6936 | g6936.t33 | TTS | g6936.t33 | 20045299 | 20045299 |
| chr_2 | g6936 | g6936.t33 | TSS | g6936.t33 | NA | NA |
Sequences
>g6936.t33 Gene=g6936 Length=746
ATTGAAAGGTCAAATTTTGTGTTGGTGGAAATTGGAAAATGAATGGATCTAAGGATTCTA
TCACAGAATTGGCCAAAATCCTTTCTGCTGGACCATTAGATCCAAATACTGAAGTCGTTG
TTGGATGCCCAAGCGTTTATATCTCATTCGCTCGAGGACTTCTTCCTTCCTCAATTGGTG
TTGCTGGCCAGAATTGCTATAAAGTCAAATCAGGAGCTTTCACAGGAGAAATCGCACCAT
CAATGTTAAAAGATGTTGGTGCTGATTGGGTTATTCTTGGACACTCAGAGAGACGTGCAA
TTTTCGGCGAAAGTGATGAATTAATCGCAGATAAAGTTGAATTTGCCCTTTCAGAAGGAT
TGAAAGTCATTGCTTGCATTGGTGAAACTCTTCAAGAACGTGAGGCTGGTCAAACTGAAG
CAGTATGCTTCCGTCAAACGAAGGCAATAGCTTCTGTTGTCAAAGACTGGTCAAATGTTG
TTATCGCTTATGAACCGGTTTGGGCAATTGGAACTGGCAAAACTGCAACTCCAGCTCAAG
CCCAAGAGGTTCATGCTGCTCTCCGTAAATGGTTCGCCGAGAACGTTTCTCAAGCCGTTT
CTGATTCAATTCGTATTCAATATGGTGGCTCAGTTACTGCTGCTAATGCTCGTGAATTAG
CTTCACAACCAGATATCGATGGATTTTTAGTTGGAGGTGCATCATTAAAACCCGAATTCG
TACAAATTGTCAATGCTAGGCAGTAA
>g6936.t33 Gene=g6936 Length=235
MNGSKDSITELAKILSAGPLDPNTEVVVGCPSVYISFARGLLPSSIGVAGQNCYKVKSGA
FTGEIAPSMLKDVGADWVILGHSERRAIFGESDELIADKVEFALSEGLKVIACIGETLQE
REAGQTEAVCFRQTKAIASVVKDWSNVVIAYEPVWAIGTGKTATPAQAQEVHAALRKWFA
ENVSQAVSDSIRIQYGGSVTAANARELASQPDIDGFLVGGASLKPEFVQIVNARQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 7 | g6936.t33 | CDD | cd00311 | TIM | 1 | 232 | 6.7331E-126 |
| 6 | g6936.t33 | Gene3D | G3DSA:3.20.20.70 | Aldolase class I | 1 | 235 | 2.2E-99 |
| 4 | g6936.t33 | Hamap | MF_00147_B | Triosephosphate isomerase [tpiA]. | 1 | 233 | 55.998657 |
| 2 | g6936.t33 | PANTHER | PTHR21139:SF17 | TRIOSEPHOSPHATE ISOMERASE A | 1 | 234 | 2.2E-108 |
| 3 | g6936.t33 | PANTHER | PTHR21139 | TRIOSEPHOSPHATE ISOMERASE | 1 | 234 | 2.2E-108 |
| 1 | g6936.t33 | Pfam | PF00121 | Triosephosphate isomerase | 1 | 231 | 6.4E-84 |
| 8 | g6936.t33 | ProSitePatterns | PS00171 | Triosephosphate isomerase active site. | 150 | 160 | - |
| 10 | g6936.t33 | ProSiteProfiles | PS51440 | Triosephosphate isomerase (TIM) family profile. | 1 | 233 | 79.575 |
| 5 | g6936.t33 | SUPERFAMILY | SSF51351 | Triosephosphate isomerase (TIM) | 1 | 233 | 1.31E-90 |
| 9 | g6936.t33 | TIGRFAM | TIGR00419 | tim: triose-phosphate isomerase | 20 | 226 | 7.9E-71 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006096 | glycolytic process | BP |
| GO:0004807 | triose-phosphate isomerase activity | MF |
| GO:0003824 | catalytic activity | MF |
KEGG
Orthology
- This transcript has been annotated with K01803 (triosephosphate isomerase (TIM))
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
