General information for g7051.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as High affinity cAMP-specific 3’,5’-cyclic phosphodiesterase 7A .
Parent gene
- The parent gene g7051 is located on chr_2: 20852130..20856477 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7051 | g7051.t1 | isoform | g7051.t1 | 20849252 | 20855584 |
| chr_2 | g7051 | g7051.t1 | exon | g7051.t1.exon1 | 20849252 | 20849336 |
| chr_2 | g7051 | g7051.t1 | cds | g7051.t1.CDS1 | 20849252 | 20849336 |
| chr_2 | g7051 | g7051.t1 | exon | g7051.t1.exon2 | 20849863 | 20850147 |
| chr_2 | g7051 | g7051.t1 | cds | g7051.t1.CDS2 | 20849863 | 20850147 |
| chr_2 | g7051 | g7051.t1 | exon | g7051.t1.exon3 | 20850232 | 20852064 |
| chr_2 | g7051 | g7051.t1 | cds | g7051.t1.CDS3 | 20850232 | 20852064 |
| chr_2 | g7051 | g7051.t1 | exon | g7051.t1.exon4 | 20852130 | 20852549 |
| chr_2 | g7051 | g7051.t1 | cds | g7051.t1.CDS4 | 20852130 | 20852549 |
| chr_2 | g7051 | g7051.t1 | exon | g7051.t1.exon5 | 20855568 | 20855584 |
| chr_2 | g7051 | g7051.t1 | cds | g7051.t1.CDS5 | 20855568 | 20855584 |
| chr_2 | g7051 | g7051.t1 | TTS | g7051.t1 | 20856534 | 20856534 |
| chr_2 | g7051 | g7051.t1 | TSS | g7051.t1 | NA | NA |
Sequences
>g7051.t1 Gene=g7051 Length=2640
ATGTTGCGATTTGTGCTGTTGTTGTGCGTTTGTCTTGCAAATATTTTCGTTATAAACACA
ACGCAAACTTGTCTGAAGCAAGCAACCGAAATACCACCTAATAACAGACGATTGTCAATA
TCAGATAAAGATCGTCCATTGATTGATGCGCTAATAGTTGAAGAAAATACATTAAGTCCA
AAAAAGCGTCACGTTTGTAACGGTCGCTTCCTTACAATGCACAAACGACGAAGGAAGAAG
CTGATGACACGCAGTTTAGCCTTTGAACCAGCAATTATCGATGATGTCTTGCATGGGCAA
GTGAAGTGTATATTGAGACAAGTGGGCAATTGGCGTTTTAATGCATTCACATTGGAGACA
GTTACTGGAGGACGATCTTTGCCTGTATTATGTGTTCACTTGTTTCAGTTGTACGGCCTT
TTGGACCATTTTAATCTAGATGTAGTTAGATTATGGAAATTATTTAGTTTAATTGAAGAG
GGTTATCACAGTACGAACCCATATCATAATTCGATTCACGCGACCGATGTCACACAAGCT
ATGCACTGTTTCCTACAGGAGACAAAAATTTATGAGCACTTGACACCACTCGAAATAATG
TCATCACTGTTGGGCGCCGTTGCTCACGATCTCGATCATCCGGGTGTTAATCAACCGTTT
CTTATAGCGACATCCAATCATTTAGCAGCACTTTACGAAAATACATCTGTGCTCGAAAAT
CACCATTGGCGTTCAGCAGTTGGCTGTCTGCTCGAAAGTGGCGTCGCTGAACAAATCGAA
GACTTTCGTCATGAAATGGAAAAGCAAATCAGCTCTCTTATCCTAGCGACCGACATTACT
CGTCAACAAGAATTTATAGGAAAATTTAAAGACTTTTTAAACAATAATACATTAAATATG
CGCAAGGCAGATCATAGACATTTTATACTACAAATAGCATTAAAGTGTGCCGATATATCT
AATCCATGCCGGCCATGGGATATATCGAAAAAATGGAGTCTGAAAGTATGCGAAGAATTT
TATCGTCAGGGTGACTATGAACGACAGCTCAATTTGCCAGTTACGTCACTCTGCGATCGT
CAGACAACCACCGTTCCAAAAATACAAACGGGCTTTTTTAAGTTCGTTGTAACGCCGCTC
ATGGCTGAGTGGCATCGCTTCCTTAAAACTGAACTATCATATCGCATGATGCGTCATCTC
AAATATAATGAAAAGCAATGGGATAGCAGACTGCAAGCCGAGTTGGCAGAAGAGACCCGC
ACTGAAATCTCTGATGCTGAAGTACTCGAAGAAGACGTCGATGAGGGTGATGATGATTCA
CGAATTGAATTTGCCGGCAGTCCAGAAATGATTCTACCCGAAATAAAATCATCGCGTCGA
AGTTCACTACAAATTCCCACTTATTCTCTTAAAGATCGCGAACGACGTCTTTCTGTGCCG
ATTCATCCTGTGCCGAAAATCATTTTACCACCACGTGACCATATGATGCGACCAGCTGAC
GCTTTATTAGAAAGCGATGAAGTTGAACATAAGTTTGATCAAGATTCACTGTCACTCTTA
TCATCAGACAGCGAGAGTGGTCAACGTGCACGCTCTTCCATCGATAGCAACGACAATGAA
CGACCATTGAGTGCGGAAAATCTTCTTCCTGACCTCAACATTGCTTCCATGACAGCAAGT
GCGTGTGCAGACAAGCTCAATATTGTCATGCACGGTTCAACCAACTTTCCTCCGCATATG
CTGGGCGTTTCAACATCCAAACATTTGGTGCGTCAACAAACTTTCCCTCCACTACAGCCA
TACGTGCGTGCACGTTATATGTCCTCAACAGCTGACTTAGGCACTTGTCCTGAAGCACTT
TTCGAAAGTCATTCTGTCAGCAGTAGTTCCGATTCAGCAGGTTGTGAAAATGATACAAAC
AGCTCAAATAGTGCGTCAAAGAAAGACAATTTAAAACGTGAACCTGAACAGGTTCCTGAT
AAGGCATCGAAATTGCCTAAACTATCTATTGGAATGGGCCAGAAAGAAAATCTTGATCCA
ACAATGCATAGGAAAAGCTCAACTAGTCGTCGACGTGGATCTGCCCCTGTGACTGTTGCA
CTTCCAACAAAATCAGGCGATCAAGATGTTTCATTGTCATATGTGATCAAGAACTCTGAG
AAGGGACGTCGATGCTCAGTTCCTGCTGAAACATTAACATTGAGTGCCAAAGATTTTGTT
GGTTCACCGAGTACTCATCCTGTGATATCATCTACTAATACATCTACGAATGCAACACAA
GCACAACAGCAGCAACAACAACAGCCAACACAGCAACAGCAGCAACAAAGACGCGGTTCG
TGTGAGATGCCAAACCTTATGATGCGCGGCAGTGTCAACGGTAAGCGACGTAACCCCAAA
AAGATCCTTAGACGTCGTTCGTCCAGTGGAGCAATTGAAATTCTCACGCCAATTGTAAGC
GAGTCAATGGAATCAACTTCAAGTGCCGGTAGTGTCACGGGCTCTGCAAATTTATGGTAT
CGTTTCAAAAAAGAATGGACATTATCGCGTCGCAATGATTCTGATACATTGCTTACAAAA
CGACGCGGTTCTCTTCCCATCGAGGTGCTCGCAATCGGCTTAGGCACATTACGTCATTAG
>g7051.t1 Gene=g7051 Length=879
MLRFVLLLCVCLANIFVINTTQTCLKQATEIPPNNRRLSISDKDRPLIDALIVEENTLSP
KKRHVCNGRFLTMHKRRRKKLMTRSLAFEPAIIDDVLHGQVKCILRQVGNWRFNAFTLET
VTGGRSLPVLCVHLFQLYGLLDHFNLDVVRLWKLFSLIEEGYHSTNPYHNSIHATDVTQA
MHCFLQETKIYEHLTPLEIMSSLLGAVAHDLDHPGVNQPFLIATSNHLAALYENTSVLEN
HHWRSAVGCLLESGVAEQIEDFRHEMEKQISSLILATDITRQQEFIGKFKDFLNNNTLNM
RKADHRHFILQIALKCADISNPCRPWDISKKWSLKVCEEFYRQGDYERQLNLPVTSLCDR
QTTTVPKIQTGFFKFVVTPLMAEWHRFLKTELSYRMMRHLKYNEKQWDSRLQAELAEETR
TEISDAEVLEEDVDEGDDDSRIEFAGSPEMILPEIKSSRRSSLQIPTYSLKDRERRLSVP
IHPVPKIILPPRDHMMRPADALLESDEVEHKFDQDSLSLLSSDSESGQRARSSIDSNDNE
RPLSAENLLPDLNIASMTASACADKLNIVMHGSTNFPPHMLGVSTSKHLVRQQTFPPLQP
YVRARYMSSTADLGTCPEALFESHSVSSSSDSAGCENDTNSSNSASKKDNLKREPEQVPD
KASKLPKLSIGMGQKENLDPTMHRKSSTSRRRGSAPVTVALPTKSGDQDVSLSYVIKNSE
KGRRCSVPAETLTLSAKDFVGSPSTHPVISSTNTSTNATQAQQQQQQQPTQQQQQQRRGS
CEMPNLMMRGSVNGKRRNPKKILRRRSSSGAIEILTPIVSESMESTSSAGSVTGSANLWY
RFKKEWTLSRRNDSDTLLTKRRGSLPIEVLAIGLGTLRH
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 19 | g7051.t1 | CDD | cd00077 | HDc | 168 | 341 | 8.09461E-8 |
| 13 | g7051.t1 | Gene3D | G3DSA:1.10.1300.10 | Catalytic domain of cyclic nucleotide phosphodiesterase 4b2b | 89 | 410 | 4.1E-117 |
| 26 | g7051.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 517 | 543 | - |
| 23 | g7051.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 626 | 646 | - |
| 25 | g7051.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 626 | 704 | - |
| 28 | g7051.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 647 | 661 | - |
| 24 | g7051.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 742 | 781 | - |
| 27 | g7051.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 742 | 799 | - |
| 2 | g7051.t1 | PANTHER | PTHR11347 | CYCLIC NUCLEOTIDE PHOSPHODIESTERASE | 67 | 421 | 1.0E-150 |
| 3 | g7051.t1 | PANTHER | PTHR11347:SF205 | 5’-CYCLIC NUCLEOTIDE PHOSPHODIESTERASE, PUTATIVE-RELATED | 67 | 421 | 1.0E-150 |
| 9 | g7051.t1 | PRINTS | PR00387 | 3’5’-cyclic nucleotide phosphodiesterase signature | 164 | 177 | 5.5E-23 |
| 8 | g7051.t1 | PRINTS | PR00387 | 3’5’-cyclic nucleotide phosphodiesterase signature | 195 | 208 | 5.5E-23 |
| 6 | g7051.t1 | PRINTS | PR00387 | 3’5’-cyclic nucleotide phosphodiesterase signature | 209 | 224 | 5.5E-23 |
| 7 | g7051.t1 | PRINTS | PR00387 | 3’5’-cyclic nucleotide phosphodiesterase signature | 236 | 252 | 5.5E-23 |
| 5 | g7051.t1 | PRINTS | PR00387 | 3’5’-cyclic nucleotide phosphodiesterase signature | 314 | 327 | 5.5E-23 |
| 4 | g7051.t1 | PRINTS | PR00387 | 3’5’-cyclic nucleotide phosphodiesterase signature | 331 | 347 | 5.5E-23 |
| 1 | g7051.t1 | Pfam | PF00233 | 3’5’-cyclic nucleotide phosphodiesterase | 168 | 392 | 2.5E-76 |
| 15 | g7051.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 20 | - |
| 16 | g7051.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 17 | g7051.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 12 | - |
| 18 | g7051.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 13 | 20 | - |
| 14 | g7051.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 21 | 879 | - |
| 22 | g7051.t1 | ProSitePatterns | PS00126 | 3’5’-cyclic nucleotide phosphodiesterase domain signature. | 209 | 220 | - |
| 29 | g7051.t1 | ProSiteProfiles | PS51845 | 3’5’-cyclic nucleotide phosphodiesterase domain profile. | 85 | 414 | 102.12 |
| 21 | g7051.t1 | SMART | SM00471 | hd_13 | 166 | 332 | 8.0E-6 |
| 10 | g7051.t1 | SUPERFAMILY | SSF109604 | HD-domain/PDEase-like | 94 | 424 | 6.28E-104 |
| 12 | g7051.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 20 | - |
| 20 | g7051.t1 | SignalP_GRAM_NEGATIVE | SignalP-noTM | SignalP-noTM | 1 | 23 | - |
| 11 | g7051.t1 | SignalP_GRAM_POSITIVE | SignalP-TM | SignalP-TM | 1 | 27 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008081 | phosphoric diester hydrolase activity | MF |
| GO:0007165 | signal transduction | BP |
| GO:0004114 | 3’,5’-cyclic-nucleotide phosphodiesterase activity | MF |
KEGG
Orthology
- This transcript has been annotated with K18436 (high affinity cAMP-specific 3’,5’-cyclic phosphodiesterase 7)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
