General information for g7096.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Diacylglycerol kinase epsilon.
Parent gene
- The parent gene g7096 is located on chr_2: 21176692..21178499 (+ strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7096 | g7096.t3 | TSS | g7096.t3 | 21176627 | 21176627 |
| chr_2 | g7096 | g7096.t3 | isoform | g7096.t3 | 21176692 | 21178499 |
| chr_2 | g7096 | g7096.t3 | exon | g7096.t3.exon1 | 21176692 | 21177454 |
| chr_2 | g7096 | g7096.t3 | cds | g7096.t3.CDS1 | 21176692 | 21177454 |
| chr_2 | g7096 | g7096.t3 | exon | g7096.t3.exon2 | 21177544 | 21177632 |
| chr_2 | g7096 | g7096.t3 | cds | g7096.t3.CDS2 | 21177544 | 21177632 |
| chr_2 | g7096 | g7096.t3 | exon | g7096.t3.exon3 | 21177704 | 21178317 |
| chr_2 | g7096 | g7096.t3 | cds | g7096.t3.CDS3 | 21177704 | 21177727 |
| chr_2 | g7096 | g7096.t3 | exon | g7096.t3.exon4 | 21178380 | 21178499 |
| chr_2 | g7096 | g7096.t3 | TTS | g7096.t3 | 21178664 | 21178664 |
Sequences
>g7096.t3 Gene=g7096 Length=1586
ATGGATTTTTATTTAATTGTGCTAATCTTTCTTATCTTACTTGTTCTTTGGATTATATTA
CACTATTATGCTAAGCCAATCATTGTCATAAATGATAGGAAGAAGCGACATTTGTGGAAG
AATAAAAATGAAAGTGATCATTTTATTTTATTTTGCAACATCTGCGAACATCTTTGTTCT
GCTTCTTCTTTCATCTGTGAATATTGCTCTATTGCGTGTGATAGAATTGAGTGTACGAAG
ATTGCGGATAAATCTTTAAAATGTAAGCAGGAAAAAGAAAGACGTGAATTGAAATCAACT
GAAGTGAAGCATCATTATATTAAAGGAAATCTTATTGAAACAGTGTGTAGCTCTTGTAAT
TTAGAAATTGAAGGTGTTCATGATGTAGGCATACATGGTACTAAGTGTTGCTGGTGTCAT
AATAGCTTTCATAATTCATGTGCAAAGAGTGAATTAGTTTGTAATTTTGGAAAATTACGA
GATTTTGTTATACCACCATTCTCTGTAAGAGCAGCGAGAACAAGGAGTGCTCCAAAATTG
CATCTAAATGAAATAACACCAATTCCTGAATGGCCTAATTGGCAACCACTAATTGTAATA
GCTAATAAATCTTCAGGCAGCAATGAAGCTGATAATATAGCAACTTTATTCAAAAGAGTT
CTTAATCCCATTCAAGTTGTTATATTAAATAATCATGGACCTGCTGAGGCGCTAGAAATT
GTAAAACTATCGCCTGTTAAATGTCGTATTTTAGTATGTGGAGGTGATGGCTCAGTAGCG
TGGACACTTAATACAATTAGCGAGATGAAATTGGATGACAAAGTATCGATTGCAATTTGT
CCACAAGGTACTGCAATGATTTATCACGTGTATTGAACTGGGGTGCAGAAATTGAAGATA
ACGATCTACAATCTCCTTTTGAATTAATTGAAAAAATAAGGAATGCAGAAGAAATTTTTT
TAGATAGATGGCTCATTGAAACAAAATATGATCACAGAACAATGATCACAAGACGCTTAC
ATCACGACAAAAAAACATTTATGTACAACTATTTTAGTGTCGGCGTTGATGCATTAGTAA
CTTTAAATTTCCATAAAGCTCGTGCGAGTGCTCTCTATGTGATTAAAAGCAAAATCATTA
ACAAGTTTCTGTATTTTATTTATGGCACTCAACAAGTTATAATGCAAGACTGTGACGGTC
TTCATGAGTATTTGGAATTATACATAAATGATATAGAGCAAGAACTCCCTGATTTACAAA
GTGTTATCTGCTTGAACATAAATTCTTGGGGTGGTGGAGTAAAATTAGTGGATATAATAA
AGGAGCATGATAAAGTATTTGCCGAAGGTCATTCGATAAATGATGAGGCGTTAGAAATTT
TTGGAATATCATCTTCTTTCCATATTGCGCAATTGCAAGTTGGTTTAACTAAACCGATTA
AATTGGGAAAGGCTAGAGAACTCAAGGTGAAACTAAAAAAAGTTCTTCCTATTCAAATTG
ATGGTGAACCTCACACTGAGATACCTTGTGAAATTAAAATCTCAGCACATAGTCAAGCGA
GGATGTTAAGGAGAAGAATAAATTAA
>g7096.t3 Gene=g7096 Length=291
MDFYLIVLIFLILLVLWIILHYYAKPIIVINDRKKRHLWKNKNESDHFILFCNICEHLCS
ASSFICEYCSIACDRIECTKIADKSLKCKQEKERRELKSTEVKHHYIKGNLIETVCSSCN
LEIEGVHDVGIHGTKCCWCHNSFHNSCAKSELVCNFGKLRDFVIPPFSVRAARTRSAPKL
HLNEITPIPEWPNWQPLIVIANKSSGSNEADNIATLFKRVLNPIQVVILNNHGPAEALEI
VKLSPVKCRILVCGGDGSVAWTLNTISEMKLDDKVSIAICPQGTAMIYHVY
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 6 | g7096.t3 | Gene3D | G3DSA:3.30.60.20 | - | 101 | 155 | 1.2E-6 |
| 5 | g7096.t3 | Gene3D | G3DSA:3.40.50.10330 | - | 194 | 288 | 9.1E-8 |
| 2 | g7096.t3 | PANTHER | PTHR11255 | DIACYLGLYCEROL KINASE | 7 | 284 | 1.7E-49 |
| 3 | g7096.t3 | PANTHER | PTHR11255:SF45 | DIACYLGLYCEROL KINASE EPSILON | 7 | 284 | 1.7E-49 |
| 1 | g7096.t3 | Pfam | PF00781 | Diacylglycerol kinase catalytic domain | 196 | 285 | 3.3E-14 |
| 8 | g7096.t3 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 1 | 5 | - |
| 9 | g7096.t3 | Phobius | TRANSMEMBRANE | Region of a membrane-bound protein predicted to be embedded in the membrane. | 6 | 24 | - |
| 7 | g7096.t3 | Phobius | CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the cytoplasm. | 25 | 291 | - |
| 12 | g7096.t3 | ProSiteProfiles | PS50081 | Zinc finger phorbol-ester/DAG-type profile. | 103 | 154 | 9.019 |
| 13 | g7096.t3 | ProSiteProfiles | PS50146 | DAG-kinase catalytic (DAGKc) domain profile. | 192 | 285 | 16.618 |
| 11 | g7096.t3 | SMART | SM00046 | dagk_c4a_7 | 196 | 289 | 1.3E-4 |
| 4 | g7096.t3 | SUPERFAMILY | SSF111331 | NAD kinase/diacylglycerol kinase-like | 192 | 285 | 1.02E-14 |
| 10 | g7096.t3 | TMHMM | TMhelix | Region of a membrane-bound protein predicted to be embedded in the membrane. | 5 | 24 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0004143 | diacylglycerol kinase activity | MF |
| GO:0003951 | NAD+ kinase activity | MF |
| GO:0007165 | signal transduction | BP |
| GO:0016301 | kinase activity | MF |
| GO:0035556 | intracellular signal transduction | BP |
KEGG
Orthology
This gene did not have any KEGG ortholog annotations (KAAS, GHOSTZ).
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
