General information for g7124.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Protein 5NUC.
Parent gene
- The parent gene g7124 is located on chr_2: 21354237..21356774 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7124 | g7124.t1 | TTS | g7124.t1 | 21351864 | 21351864 |
| chr_2 | g7124 | g7124.t1 | isoform | g7124.t1 | 21352328 | 21355223 |
| chr_2 | g7124 | g7124.t1 | exon | g7124.t1.exon1 | 21352328 | 21352510 |
| chr_2 | g7124 | g7124.t1 | cds | g7124.t1.CDS1 | 21352328 | 21352510 |
| chr_2 | g7124 | g7124.t1 | exon | g7124.t1.exon2 | 21352575 | 21352813 |
| chr_2 | g7124 | g7124.t1 | cds | g7124.t1.CDS2 | 21352575 | 21352813 |
| chr_2 | g7124 | g7124.t1 | exon | g7124.t1.exon3 | 21352877 | 21352960 |
| chr_2 | g7124 | g7124.t1 | cds | g7124.t1.CDS3 | 21352877 | 21352960 |
| chr_2 | g7124 | g7124.t1 | exon | g7124.t1.exon4 | 21353022 | 21353264 |
| chr_2 | g7124 | g7124.t1 | cds | g7124.t1.CDS4 | 21353022 | 21353264 |
| chr_2 | g7124 | g7124.t1 | exon | g7124.t1.exon5 | 21353325 | 21353750 |
| chr_2 | g7124 | g7124.t1 | cds | g7124.t1.CDS5 | 21353325 | 21353750 |
| chr_2 | g7124 | g7124.t1 | exon | g7124.t1.exon6 | 21353829 | 21354254 |
| chr_2 | g7124 | g7124.t1 | cds | g7124.t1.CDS6 | 21353829 | 21354254 |
| chr_2 | g7124 | g7124.t1 | exon | g7124.t1.exon7 | 21354331 | 21354736 |
| chr_2 | g7124 | g7124.t1 | cds | g7124.t1.CDS7 | 21354331 | 21354736 |
| chr_2 | g7124 | g7124.t1 | exon | g7124.t1.exon8 | 21355110 | 21355223 |
| chr_2 | g7124 | g7124.t1 | cds | g7124.t1.CDS8 | 21355110 | 21355223 |
| chr_2 | g7124 | g7124.t1 | TSS | g7124.t1 | NA | NA |
Sequences
>g7124.t1 Gene=g7124 Length=2121
ATGGCAGGCTTTGCGAATCAAGCGGCGCTTCGAAGTCATTATGAAGACTTGATAGATTCA
ACAGAGTCGGATATTTCAGAAGGTCTAAAAAATGCAGTGTCATGGTTCAAACAGGCATCA
GCAGAAGTTCGTGAGGCTGGGAAGCGTGCAGTCGTACAAATTCAAACTAAAAATCCAAAT
TTAGTAGTTTCACCAGGCACTTCAAAAACTCAAATAATTAAGTCATTACTCAAGCCAATT
AGTCCCTCGAAATCTAATGCATCTAGTAGTAGCAGCATTAGCAGTGAAATGGGCGCCATG
AAAGATCAATTAACAACTAGAGGAGAAGAAGATAACGTAAAAAAGTTAACAATACTACAT
TACAACGATGTATATAACATTGATCAGCAAGTGAAATCAGAGCCAATTGGAGGCGCGGCG
CGTTTCACAACAGCAATTCATTCATTTAAACATTTGAATCCACTTGTGCTGTTTAGTGGT
GACGCTTTTAATCCCTCTATGCTTTCGACATTTACACAAGGTGAACAAATGATTCCAGTA
TTGAATAAAGTCGAAACGGCGTGTGCTGTTTTTGGGAATCATGATTTCGATCATGGCTTA
GATGTGCTTCAAAAATGGGTGGAACAGACGAACTTTCCTTGGCTCATGTCAAATGTCATT
GATAATGAAACAGGCCGACCACTGGGGAATGGCAAAATCAGTCACATTATATGTCATAAT
GATTTAAAAATTGGCTTAATGGGATTAGTTGAGAAGGAATGGCTTGATACCTTGCCAACT
ATTGATCCAAATGAAGTGACATACATCGATTATGTGAAAGCGGGTAATCAACTTGCTGAT
GATCTTCTTAATGAAGGATGTGATATAATAATTGCTCTCACACACATGCGTACACCAAAT
GACATAAATCTCGCACAAAACTGTCCCAAATTGTCATTAATTTTAGGTGGTCATGATCAT
GTGTATGAAATTGTTAAAGTGAATAATTTGAATGTAATCAAATCGGGCACTGATTTTAGA
CAATTCTCAAAAATTACAATAAAAAAGTCGTTGAAAAATAATGAGCCACATTTAGACGTT
GATATTGAAAAGATTGATGTTACTTCTGCATTCGAAGAGGATTTAGATTTGAAAGCCGAA
TTAGCTCACTATAGTGAGATGATAGAATCAAAAATGGGAATTGTACTGGGTAATTTCTCA
GTTGAGCTTGATGCTCGTTTTGCAAAAATAAGAACAAGTGAAACTAATTTGGGAAATTGG
ATTACGGACATTGCTTTAGCAGCAACATGTGCGGACGTAGTTATTATCAACAGCGGAACA
TTTAGAAGCGATCAAATTCATGAAGCTGGTCCGTTTACAATGAAAGATCTCGTTTCAATT
ATTCCGATGCAGGACCCCTTGATTGTGATCGAAGTGACAGGAAAAATTTTGCATGAAGCT
CTTGAGAATGCTGTGTCGGCTTATCCTAAATTAGAAGGTCGTTTTCCTCAAGTATCTGGT
ATATCATTCACTTTCAACCCCGATAGACCTCCAGGAAATCGTGTTGAACATCGACTTGTT
CGCATAGGTGATGAATGGTTGAAACTTGATGAAAAATACACATTGTGCATTAAAGCATAT
ATGCATGGAGGATGTGATGGATATACTATGTTTAAAAATTGTCCAATTCTAATGGATGAA
GATGCTTGTCCTGAATTAGGCCTTGCAATACAAAATCATTTTAAAGCAATAGATGTAAAG
ACCGGAAAAACAGGAAAGCATTCAAAACATCGTCAAAGTCTTGTTACAATTTCTCGAAGA
CACAGTATGATTCAAATGCTTGAAAACCTTGAACTTGATGGACCCTGCCCTGTTCGCCTG
CATCATCAACAACAATCAAGCAATTCACAACAACAGCAACCTCCTCCACCTCAACAGAAT
ATTTCTACAAGTGCAAAGCTGCTGAGACGAGCATCACTCGATGACTTAGAGCAGTCGTCT
TGTCAACTATGTCCCACAATTCAACATCGAATAGTGATGGTTCAAAATGAAGAACATTTA
AAGCAGATGCTGCTTAGACGCGAAGCAATGGAAATGACAACTGAAATCATAAACGAATTG
GATGAAAACAGTCCACAATAA
>g7124.t1 Gene=g7124 Length=706
MAGFANQAALRSHYEDLIDSTESDISEGLKNAVSWFKQASAEVREAGKRAVVQIQTKNPN
LVVSPGTSKTQIIKSLLKPISPSKSNASSSSSISSEMGAMKDQLTTRGEEDNVKKLTILH
YNDVYNIDQQVKSEPIGGAARFTTAIHSFKHLNPLVLFSGDAFNPSMLSTFTQGEQMIPV
LNKVETACAVFGNHDFDHGLDVLQKWVEQTNFPWLMSNVIDNETGRPLGNGKISHIICHN
DLKIGLMGLVEKEWLDTLPTIDPNEVTYIDYVKAGNQLADDLLNEGCDIIIALTHMRTPN
DINLAQNCPKLSLILGGHDHVYEIVKVNNLNVIKSGTDFRQFSKITIKKSLKNNEPHLDV
DIEKIDVTSAFEEDLDLKAELAHYSEMIESKMGIVLGNFSVELDARFAKIRTSETNLGNW
ITDIALAATCADVVIINSGTFRSDQIHEAGPFTMKDLVSIIPMQDPLIVIEVTGKILHEA
LENAVSAYPKLEGRFPQVSGISFTFNPDRPPGNRVEHRLVRIGDEWLKLDEKYTLCIKAY
MHGGCDGYTMFKNCPILMDEDACPELGLAIQNHFKAIDVKTGKTGKHSKHRQSLVTISRR
HSMIQMLENLELDGPCPVRLHHQQQSSNSQQQQPPPPQQNISTSAKLLRRASLDDLEQSS
CQLCPTIQHRIVMVQNEEHLKQMLLRREAMEMTTEIINELDENSPQ
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 14 | g7124.t1 | CDD | cd07406 | MPP_CG11883_N | 116 | 374 | 2.95213E-133 |
| 12 | g7124.t1 | Gene3D | G3DSA:3.60.21.10 | - | 106 | 392 | 3.3E-57 |
| 13 | g7124.t1 | Gene3D | G3DSA:3.90.780.10 | - | 395 | 576 | 3.5E-46 |
| 11 | g7124.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 620 | 642 | - |
| 3 | g7124.t1 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 114 | 686 | 3.2E-159 |
| 4 | g7124.t1 | PANTHER | PTHR11575:SF24 | RIKEN CDNA 4933425L06 | 114 | 686 | 3.2E-159 |
| 6 | g7124.t1 | PRINTS | PR01607 | Apyrase family signature | 114 | 132 | 1.4E-10 |
| 8 | g7124.t1 | PRINTS | PR01607 | Apyrase family signature | 285 | 302 | 1.4E-10 |
| 7 | g7124.t1 | PRINTS | PR01607 | Apyrase family signature | 304 | 327 | 1.4E-10 |
| 5 | g7124.t1 | PRINTS | PR01607 | Apyrase family signature | 451 | 474 | 1.4E-10 |
| 2 | g7124.t1 | Pfam | PF00149 | Calcineurin-like phosphoesterase | 117 | 322 | 3.5E-9 |
| 1 | g7124.t1 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 395 | 552 | 4.8E-38 |
| 10 | g7124.t1 | SUPERFAMILY | SSF56300 | Metallo-dependent phosphatases | 111 | 391 | 3.45E-63 |
| 9 | g7124.t1 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 393 | 557 | 7.32E-44 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0009166 | nucleotide catabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K01081 (5’-nucleotidase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
