General information for g7124.t3
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Putative Trifunctional nucleotide phosphoesterase protein YfkN.
Parent gene
- The parent gene g7124 is located on chr_2: 21354237..21356774 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7124 | g7124.t3 | isoform | g7124.t3 | 21352970 | 21356774 |
| chr_2 | g7124 | g7124.t3 | exon | g7124.t3.exon1 | 21352970 | 21353264 |
| chr_2 | g7124 | g7124.t3 | cds | g7124.t3.CDS1 | 21352996 | 21353264 |
| chr_2 | g7124 | g7124.t3 | exon | g7124.t3.exon2 | 21353325 | 21353750 |
| chr_2 | g7124 | g7124.t3 | cds | g7124.t3.CDS2 | 21353325 | 21353750 |
| chr_2 | g7124 | g7124.t3 | exon | g7124.t3.exon3 | 21353829 | 21354254 |
| chr_2 | g7124 | g7124.t3 | cds | g7124.t3.CDS3 | 21353829 | 21354254 |
| chr_2 | g7124 | g7124.t3 | exon | g7124.t3.exon4 | 21354331 | 21354736 |
| chr_2 | g7124 | g7124.t3 | cds | g7124.t3.CDS4 | 21354331 | 21354562 |
| chr_2 | g7124 | g7124.t3 | exon | g7124.t3.exon5 | 21356494 | 21356774 |
| chr_2 | g7124 | g7124.t3 | TSS | g7124.t3 | NA | NA |
| chr_2 | g7124 | g7124.t3 | TTS | g7124.t3 | NA | NA |
Sequences
>g7124.t3 Gene=g7124 Length=1834
TTAGAGTCGTTTAATAATAAATCCAAAAAAAAATTTGTAGAACAGTCGAAAGTATGTTGA
GATTTTAAAATAAACAGTAAGAATATAATCTGTGATTTGCTACAAGGAAAAAAAATCAAA
TTGTATCCAGGGCGCACTTTCTTTGAGAAAAAAAGTGTCTATACACTGTACAGTTTATTT
AATTTATTTATTTCTTAGTATTAAAGCAGTTGATTTAACATAATATCTTTATTAAAAAGC
GTCCTTATTGCTAGAGAGATTGTGATTGTTGATTAAAGAAGGCATCAGCAGAAGTTCGTG
AGGCTGGGAAGCGTGCAGTCGTACAAATTCAAACTAAAAATCCAAATTTAGTAGTTTCAC
CAGGCACTTCAAAAACTCAAATAATTAAGTCATTACTCAAGCCAATTAGTCCCTCGAAAT
CTAATGCATCTAGTAGTAGCAGCATTAGCAGTGAAATGGGCGCCATGAAAGATCAATTAA
CAACTAGAGGAGAAGAAGATAACGTAAAAAAGTTAACAATACTACATTACAACGATGTAT
ATAACATTGATCAGCAAGTGAAATCAGAGCCAATTGGAGGCGCGGCGCGTTTCACAACAG
CAATTCATTCATTTAAACATTTGAATCCACTTGTGCTGTTTAGTGGTGACGCTTTTAATC
CCTCTATGCTTTCGACATTTACACAAGGTGAACAAATGATTCCAGTATTGAATAAAGTCG
AAACGGCGTGTGCTGTTTTTGGGAATCATGATTTCGATCATGGCTTAGATGTGCTTCAAA
AATGGGTGGAACAGACGAACTTTCCTTGGCTCATGTCAAATGTCATTGATAATGAAACAG
GCCGACCACTGGGGAATGGCAAAATCAGTCACATTATATGTCATAATGATTTAAAAATTG
GCTTAATGGGATTAGTTGAGAAGGAATGGCTTGATACCTTGCCAACTATTGATCCAAATG
AAGTGACATACATCGATTATGTGAAAGCGGGTAATCAACTTGCTGATGATCTTCTTAATG
AAGGATGTGATATAATAATTGCTCTCACACACATGCGTACACCAAATGACATAAATCTCG
CACAAAACTGTCCCAAATTGTCATTAATTTTAGGTGGTCATGATCATGTGTATGAAATTG
TTAAAGTGAATAATTTGAATGTAATCAAATCGGGCACTGATTTTAGACAATTCTCAAAAA
TTACAATAAAAAAGTCGTTGAAAAATAATGAGCCACATTTAGACGTTGATATTGAAAAGA
TTGATGTTACTTCTGCATTCGAAGAGGATTTAGATTTGAAAGCCGAATTAGCTCACTATA
GTGAGATGATAGAATCAAAAATGGGAATTGTACTGGGTAATTTCTCAGTTGAGCTTGATG
CTCGTTTTGCAAAAATAAGAACAAGTGAAACTAATTTGGGAAATTGGATTACGGACATTG
CTTTAGCAGCAACATGTGCGGACGTAGTTATTATCAACAGCGGAACATTTAGAAGCGATC
AAATTCATGAAGCTGGTCCGTTTACAATGAAAGATCTCGTTTCAATTATTCCGATGCAGG
ACCCCTTGATTGTGATCGAAGTGACAGGAAAAATTTTGCATGAAGCTCTTGAGAATGCTG
TGTCGGCTTATCCTAAATTAGAAGGTCGTTTTCCTCAAGTATCTGGTATATCATTCACTT
TCAACCCCGATAGACCTCCAGGAAATCGTGTTGAACATCGACTTGTTCGCATAGGTGATG
AATGGTTGAAACTTGATGAAAAATACACATTGTGCATTAAAGGTTGTATATTTTATTTTG
AAAACTAAAAAAAAATTTACAAAAAATGTTTACA
>g7124.t3 Gene=g7124 Length=450
MGAMKDQLTTRGEEDNVKKLTILHYNDVYNIDQQVKSEPIGGAARFTTAIHSFKHLNPLV
LFSGDAFNPSMLSTFTQGEQMIPVLNKVETACAVFGNHDFDHGLDVLQKWVEQTNFPWLM
SNVIDNETGRPLGNGKISHIICHNDLKIGLMGLVEKEWLDTLPTIDPNEVTYIDYVKAGN
QLADDLLNEGCDIIIALTHMRTPNDINLAQNCPKLSLILGGHDHVYEIVKVNNLNVIKSG
TDFRQFSKITIKKSLKNNEPHLDVDIEKIDVTSAFEEDLDLKAELAHYSEMIESKMGIVL
GNFSVELDARFAKIRTSETNLGNWITDIALAATCADVVIINSGTFRSDQIHEAGPFTMKD
LVSIIPMQDPLIVIEVTGKILHEALENAVSAYPKLEGRFPQVSGISFTFNPDRPPGNRVE
HRLVRIGDEWLKLDEKYTLCIKGCIFYFEN
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g7124.t3 | CDD | cd07406 | MPP_CG11883_N | 20 | 278 | 0 |
| 11 | g7124.t3 | Gene3D | G3DSA:3.60.21.10 | - | 10 | 296 | 0 |
| 12 | g7124.t3 | Gene3D | G3DSA:3.90.780.10 | - | 299 | 443 | 0 |
| 3 | g7124.t3 | PANTHER | PTHR11575 | 5’-NUCLEOTIDASE-RELATED | 18 | 443 | 0 |
| 4 | g7124.t3 | PANTHER | PTHR11575:SF24 | RIKEN CDNA 4933425L06 | 18 | 443 | 0 |
| 7 | g7124.t3 | PRINTS | PR01607 | Apyrase family signature | 18 | 36 | 0 |
| 5 | g7124.t3 | PRINTS | PR01607 | Apyrase family signature | 189 | 206 | 0 |
| 8 | g7124.t3 | PRINTS | PR01607 | Apyrase family signature | 208 | 231 | 0 |
| 6 | g7124.t3 | PRINTS | PR01607 | Apyrase family signature | 355 | 378 | 0 |
| 2 | g7124.t3 | Pfam | PF00149 | Calcineurin-like phosphoesterase | 21 | 226 | 0 |
| 1 | g7124.t3 | Pfam | PF02872 | 5’-nucleotidase, C-terminal domain | 299 | 441 | 0 |
| 10 | g7124.t3 | SUPERFAMILY | SSF56300 | Metallo-dependent phosphatases | 15 | 295 | 0 |
| 9 | g7124.t3 | SUPERFAMILY | SSF55816 | 5’-nucleotidase (syn. UDP-sugar hydrolase), C-terminal domain | 297 | 441 | 0 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0016787 | hydrolase activity | MF |
| GO:0009166 | nucleotide catabolic process | BP |
KEGG
Orthology
- This transcript has been annotated with K11751 (5’-nucleotidase / UDP-sugar diphosphatase)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
