General information for g7134.t1
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Protein prickle.
Parent gene
- The parent gene g7134 is located on chr_2: 21409165..21410083 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_2 | g7134 | g7134.t1 | isoform | g7134.t1 | 21397553 | 21410083 |
| chr_2 | g7134 | g7134.t1 | exon | g7134.t1.exon1 | 21397553 | 21397836 |
| chr_2 | g7134 | g7134.t1 | cds | g7134.t1.CDS1 | 21397553 | 21397836 |
| chr_2 | g7134 | g7134.t1 | exon | g7134.t1.exon2 | 21397919 | 21398117 |
| chr_2 | g7134 | g7134.t1 | cds | g7134.t1.CDS2 | 21397919 | 21398117 |
| chr_2 | g7134 | g7134.t1 | exon | g7134.t1.exon3 | 21399411 | 21399614 |
| chr_2 | g7134 | g7134.t1 | cds | g7134.t1.CDS3 | 21399411 | 21399614 |
| chr_2 | g7134 | g7134.t1 | exon | g7134.t1.exon4 | 21402166 | 21402294 |
| chr_2 | g7134 | g7134.t1 | cds | g7134.t1.CDS4 | 21402166 | 21402294 |
| chr_2 | g7134 | g7134.t1 | exon | g7134.t1.exon5 | 21403101 | 21403214 |
| chr_2 | g7134 | g7134.t1 | cds | g7134.t1.CDS5 | 21403101 | 21403214 |
| chr_2 | g7134 | g7134.t1 | exon | g7134.t1.exon6 | 21407444 | 21407879 |
| chr_2 | g7134 | g7134.t1 | cds | g7134.t1.CDS6 | 21407444 | 21407879 |
| chr_2 | g7134 | g7134.t1 | exon | g7134.t1.exon7 | 21409165 | 21409696 |
| chr_2 | g7134 | g7134.t1 | cds | g7134.t1.CDS7 | 21409165 | 21409696 |
| chr_2 | g7134 | g7134.t1 | exon | g7134.t1.exon8 | 21409756 | 21409929 |
| chr_2 | g7134 | g7134.t1 | cds | g7134.t1.CDS8 | 21409756 | 21409929 |
| chr_2 | g7134 | g7134.t1 | exon | g7134.t1.exon9 | 21409993 | 21410083 |
| chr_2 | g7134 | g7134.t1 | cds | g7134.t1.CDS9 | 21409993 | 21410083 |
| chr_2 | g7134 | g7134.t1 | TSS | g7134.t1 | 21410110 | 21410110 |
| chr_2 | g7134 | g7134.t1 | TTS | g7134.t1 | NA | NA |
Sequences
>g7134.t1 Gene=g7134 Length=2163
ATGAAATCAATTTTTGTGGTGGTAGTTTTTATAACAGTTTCAACAGCAAATCCATGGCGA
GGTCTAGAGAGACCTCTGAATCAGCAGCAGATTGAGAGGCTGAGAAATATCGATGTGAAT
GTGAATGCAGAAGAGTTAAGCGGACAAGTCGAGGGTGATATGCTTATTTATTCACACGAG
TATTCATCGTTTAATGGCCGTATAGAAGCGAGTAGACGATGGCCAAACAATACTGTGCCA
TATGTCATTAACACGGCATTTTTCGATGACAACCACATTCGGTATATTCATGGTGCTGCA
CAATATTTGAATGAACGTACATGCATTCGATTTATTAATCGCACTACAGAAATTGATCAC
GTTTTCATAACTGGCGATTCAATTGGCTGTGCTGCGCAAGTTGGTCGTGTTGGTGGCGCT
CAAAGAATTCGTTTGCAACCGCATGCCATTGACACTGGCTGCTTCCGCTTCTTTACAATT
GTTCACGAGCTCATTCATGCTCTTGGTTTTCATCACATGCACAACTCTTTTGATCGTGAC
AGATATTTAAGAATTAATTGGCAAAATATTGCACCAGGCAGTGAAAGTAATTTTCAAATT
CGTCCATCAACACAGGTCACACATTTTGGCATTCCTTATGATGTTGGCAGTATCATGCAC
TATAGCTCAACAGCATTTAGCGGTAATGGACTCGATACTATGACAGCTCTATCAAACCCG
CACAGTCGTGTTATGGGACAAAGAAGTGAGGCAACGCCTGAAGATATTTTGAGAATAAAT
TTAATGTACAATTGCCTTTCTGGAAATTTCTCACCAACTTTAATAATGTCATCGTCATCG
TCATTTAGTCAAAATGGTGTTGATACTAAAACTACTACTTATAATCAACGACCACCAATG
CCATTGAGTCCGCAAAATAGCATTGGATTACCGCCATATCATTCAAATTCCACGCATGTC
TATCCACAACAGCAGCAGTCGATGGTTATAAATAATCAGCAAGAGCAGCAAAACATTCCA
TCAAAAAGTACCTTGCCAATGTACCATCATAGAATGTCAATGGCAATGCAACAACAGCAA
CAACCGCAAACACAATTTTCGCATAATCGTCTACCAATGATGGGACATCATCAACAATTG
TTGGATATTCAACGACAATCGCAATCAGATGATGACAGTGGTTGTGCACTCGAAGAATAT
ACTTGGATTCCAGCTGGTCTTCGACCTGAACAGGTGCATTTGTACTTCTCTTCGATACCA
GAAGATAAAGTGCCTTATGTGAATAGTGTAGGTGAAAGATATCGAGTTAAACAGTTATTG
CAGCAATTGCCACCACATGATAATAGCGTTCGCTATTGTCATTCACTAACTGAGGAAGAA
CGTAAAGAGTTGCAGCTCTTTTCAACACAACGAAAGCGTGATGCATTGGGACGTGGCACT
GTAAAGCAACTGGCAGTCAATCAGCAATGTGAAGGTTGCAATGATGTCATGATGTCAGGC
GATATCGCAGTTTATGCATCGCGTTTAGGAACAAATGTTTGTTGGCATCCTTCTTGCTTC
ATATGTACAGTCTGTAAAGAACTTCTGGTCGATCTAATTTATTTTCATCGTGAGGGAAAG
CTTTATTGTGGACGCCATCATGCTGAAACATTAAAACCACGCTGTTCTGCATGTGACGAA
ATAATTTTGGCGGATGAATGCACGGAGGCTGAGGGTCGTGCATGGCATATGAAGCACTTT
GCGTGTTTTGATTGCGATAAGCAGTTGGGCGGTCAGCGTTATATCATGCGAGAAGGAAAA
CCTTATTGTCTTGGATGCTTCGATAATATGTTTGCCGAGTATTGCGATTATTGTGGTGAG
ACAATCGGTGTAGATCAGGGTCGTCCATTTTTACCTCGTCGTGGTTCGATTTATTGCTCA
ATTGCGTGTAGCAAAGGTGAACCTCCAACGCCAACTGACAGCTCTGTTCCTTCTACTGCT
CGACCATTACTCAAATCAACACCTTCACAACAGATGCAACAATCAATGTCAACAAATCAG
GGATCAGATAATGATGAATCAATTGCTGCTCAATCAACACCTCCAACATCACCAAAGCTT
ACAACCTTCATCACCCATAAACAACAATCAACAACTTCAATTTGTGCAGCATCAAATGAC
TAG
>g7134.t1 Gene=g7134 Length=720
MKSIFVVVVFITVSTANPWRGLERPLNQQQIERLRNIDVNVNAEELSGQVEGDMLIYSHE
YSSFNGRIEASRRWPNNTVPYVINTAFFDDNHIRYIHGAAQYLNERTCIRFINRTTEIDH
VFITGDSIGCAAQVGRVGGAQRIRLQPHAIDTGCFRFFTIVHELIHALGFHHMHNSFDRD
RYLRINWQNIAPGSESNFQIRPSTQVTHFGIPYDVGSIMHYSSTAFSGNGLDTMTALSNP
HSRVMGQRSEATPEDILRINLMYNCLSGNFSPTLIMSSSSSFSQNGVDTKTTTYNQRPPM
PLSPQNSIGLPPYHSNSTHVYPQQQQSMVINNQQEQQNIPSKSTLPMYHHRMSMAMQQQQ
QPQTQFSHNRLPMMGHHQQLLDIQRQSQSDDDSGCALEEYTWIPAGLRPEQVHLYFSSIP
EDKVPYVNSVGERYRVKQLLQQLPPHDNSVRYCHSLTEEERKELQLFSTQRKRDALGRGT
VKQLAVNQQCEGCNDVMMSGDIAVYASRLGTNVCWHPSCFICTVCKELLVDLIYFHREGK
LYCGRHHAETLKPRCSACDEIILADECTEAEGRAWHMKHFACFDCDKQLGGQRYIMREGK
PYCLGCFDNMFAEYCDYCGETIGVDQGRPFLPRRGSIYCSIACSKGEPPTPTDSSVPSTA
RPLLKSTPSQQMQQSMSTNQGSDNDESIAAQSTPPTSPKLTTFITHKQQSTTSICAASND
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 28 | g7134.t1 | CDD | cd04280 | ZnMc_astacin_like | 76 | 263 | 1.3451E-79 |
| 27 | g7134.t1 | CDD | cd09827 | PET_Prickle | 389 | 484 | 4.3687E-66 |
| 29 | g7134.t1 | CDD | cd09415 | LIM1_Prickle | 490 | 548 | 8.17735E-35 |
| 26 | g7134.t1 | CDD | cd09418 | LIM2_Prickle | 553 | 608 | 9.52561E-37 |
| 18 | g7134.t1 | Gene3D | G3DSA:3.40.390.10 | Collagenase (Catalytic Domain) | 27 | 267 | 2.8E-67 |
| 19 | g7134.t1 | Gene3D | G3DSA:2.10.110.10 | Cysteine Rich Protein | 477 | 549 | 4.4E-14 |
| 20 | g7134.t1 | Gene3D | G3DSA:2.10.110.10 | Cysteine Rich Protein | 550 | 614 | 1.1E-18 |
| 34 | g7134.t1 | MobiDBLite | mobidb-lite | consensus disorder prediction | 649 | 720 | - |
| 6 | g7134.t1 | PANTHER | PTHR24211:SF20 | PROTEIN ESPINAS-RELATED | 326 | 628 | 1.3E-174 |
| 8 | g7134.t1 | PANTHER | PTHR24211 | LIM DOMAIN-CONTAINING PROTEIN | 326 | 628 | 1.3E-174 |
| 5 | g7134.t1 | PANTHER | PTHR24211:SF20 | PROTEIN ESPINAS-RELATED | 627 | 703 | 1.3E-174 |
| 7 | g7134.t1 | PANTHER | PTHR24211 | LIM DOMAIN-CONTAINING PROTEIN | 627 | 703 | 1.3E-174 |
| 12 | g7134.t1 | PRINTS | PR00480 | Astacin family signature | 96 | 114 | 1.2E-22 |
| 9 | g7134.t1 | PRINTS | PR00480 | Astacin family signature | 154 | 172 | 1.2E-22 |
| 11 | g7134.t1 | PRINTS | PR00480 | Astacin family signature | 173 | 190 | 1.2E-22 |
| 13 | g7134.t1 | PRINTS | PR00480 | Astacin family signature | 212 | 227 | 1.2E-22 |
| 10 | g7134.t1 | PRINTS | PR00480 | Astacin family signature | 251 | 264 | 1.2E-22 |
| 2 | g7134.t1 | Pfam | PF01400 | Astacin (Peptidase family M12A) | 72 | 265 | 1.5E-52 |
| 1 | g7134.t1 | Pfam | PF06297 | PET Domain | 398 | 482 | 1.5E-35 |
| 3 | g7134.t1 | Pfam | PF00412 | LIM domain | 490 | 549 | 2.5E-10 |
| 4 | g7134.t1 | Pfam | PF00412 | LIM domain | 555 | 610 | 9.0E-13 |
| 22 | g7134.t1 | Phobius | SIGNAL_PEPTIDE | Signal peptide region | 1 | 16 | - |
| 23 | g7134.t1 | Phobius | SIGNAL_PEPTIDE_N_REGION | N-terminal region of a signal peptide. | 1 | 3 | - |
| 24 | g7134.t1 | Phobius | SIGNAL_PEPTIDE_H_REGION | Hydrophobic region of a signal peptide. | 4 | 11 | - |
| 25 | g7134.t1 | Phobius | SIGNAL_PEPTIDE_C_REGION | C-terminal region of a signal peptide. | 12 | 16 | - |
| 21 | g7134.t1 | Phobius | NON_CYTOPLASMIC_DOMAIN | Region of a membrane-bound protein predicted to be outside the membrane, in the extracellular region. | 17 | 720 | - |
| 33 | g7134.t1 | ProSitePatterns | PS00478 | LIM zinc-binding domain signature. | 555 | 589 | - |
| 35 | g7134.t1 | ProSiteProfiles | PS51864 | Astacin-like domain profile. | 65 | 266 | 69.548 |
| 36 | g7134.t1 | ProSiteProfiles | PS51303 | PET domain profile. | 381 | 489 | 35.745 |
| 37 | g7134.t1 | ProSiteProfiles | PS50023 | LIM domain profile. | 488 | 552 | 10.009 |
| 38 | g7134.t1 | ProSiteProfiles | PS50023 | LIM domain profile. | 553 | 613 | 13.583 |
| 30 | g7134.t1 | SMART | SM00235 | col_5 | 70 | 217 | 2.4E-30 |
| 32 | g7134.t1 | SMART | SM00132 | lim_4 | 489 | 546 | 1.9E-9 |
| 31 | g7134.t1 | SMART | SM00132 | lim_4 | 554 | 606 | 2.0E-17 |
| 16 | g7134.t1 | SUPERFAMILY | SSF55486 | Metalloproteases (zincins), catalytic domain | 71 | 265 | 1.24E-49 |
| 14 | g7134.t1 | SUPERFAMILY | SSF57716 | Glucocorticoid receptor-like (DNA-binding domain) | 522 | 586 | 1.24E-14 |
| 15 | g7134.t1 | SUPERFAMILY | SSF57716 | Glucocorticoid receptor-like (DNA-binding domain) | 582 | 626 | 1.24E-10 |
| 17 | g7134.t1 | SignalP_EUK | SignalP-noTM | SignalP-noTM | 1 | 16 | - |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0008237 | metallopeptidase activity | MF |
| GO:0006508 | proteolysis | BP |
| GO:0004222 | metalloendopeptidase activity | MF |
| GO:0008270 | zinc ion binding | MF |
KEGG
Orthology
- This transcript has been annotated with K04511 (prickle)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below. There were no conditions that were differentially expressed