General information for g714.t20
Yuki Yoshida
22/1/2022
Gene loci information
Transcript annotation
- This transcript has been annotated as Elongation factor 1-gamma.
Parent gene
- The parent gene g714 is located on chr_3: 5473182..5479543 (- strand)
Gene structure
- The exon-intron structure of all isoforms are indicated below. CDS regions are colored in green. TSS and TTs that were predicted with CTR-Seq data are indicated in solid circle and squares, respectively. More specific data are shown in the table below.
| Chromosome | Gene | Transcript | Category | ID | Start | End |
|---|---|---|---|---|---|---|
| chr_3 | g714 | g714.t20 | TTS | g714.t20 | 5471464 | 5471464 |
| chr_3 | g714 | g714.t20 | isoform | g714.t20 | 5471712 | 5473474 |
| chr_3 | g714 | g714.t20 | exon | g714.t20.exon1 | 5471712 | 5472853 |
| chr_3 | g714 | g714.t20 | cds | g714.t20.CDS1 | 5471712 | 5472853 |
| chr_3 | g714 | g714.t20 | exon | g714.t20.exon2 | 5473040 | 5473206 |
| chr_3 | g714 | g714.t20 | cds | g714.t20.CDS2 | 5473040 | 5473196 |
| chr_3 | g714 | g714.t20 | exon | g714.t20.exon3 | 5473448 | 5473474 |
| chr_3 | g714 | g714.t20 | TSS | g714.t20 | 5473488 | 5473488 |
Sequences
>g714.t20 Gene=g714 Length=1336
TCTGGAATTGAAGAGTCCGTAAGCAAGATAAATCAACATGTTGCTCTACACGTATCCAGA
AAATTTCCGTGCTTATAAAGCTCTCATTGCGGCTCAATACTCGGGAGCTAAAGTTGATAT
TGCCCCAAATTTCGTTTTTGGTGAAACAAACCGTACTGAATCCTTCTTAAAGAAATTTCC
TCTTGGAAAAGTAACATTAGAAACTGATGACGGTAAATATTTAGCTGAATCAAATGCAAT
TGCATACTACGTCTCAAATGCTCAATTACGCGGTGCAAGTGATTTTGAGAAAGCGGAAGT
TCTTCAATGGTTGAGTTTTGCCGATAATGAAATTGGACCAGCTGCAGCAGCATGGGTCTA
TCCAGCTTTGGGCTACATCCAATTCAACAAGCTATCACATGAACGTGCAAAGCAAGATAT
TGCTCGTTCACTCGAAAGATTGCAAGCTCGTCTCGTAAATAATACTTATCTTGTCGGCGA
ACGCATCACACTTGCCGATATTGTTGTTTTCACTAGCCTCCTTTCATTATTCCAACACGT
TGCTGATCCAGCATATCGCAAGCCATTCTTTGCTGTCAATCGTTGGTTCAATACAATTCT
CAATCAGCCACAAACAAAAGCAGTTGTTAAGAATTTCACTTTCTGTGAAAAAGAAACACC
ATTCGATCCTAAGAAATATGCCGAATTCCAAGCCAAATTTGGCGGTGGTGCTCAACAAAA
GGAAAAGAAGGAAAAAGAAAAGAAAGAGAAACAACCACAACAACCAGCAGCTGAGAAGAA
AAAGAAGGAGAAAGAAGCAGAACCTGCTGAAGAGATGGATGAAGCAGAATTGGCACTTGC
TGCTGAACCAAAATCAAAAGATCCATTTGATTCTCTCCCAAAAGGCACATTCAATATGGA
CGACTTCAAGCGTGTATACAGTAATGAAGATGAAGCAAAATCAATTCCATATTTCTTCGA
GAAATTCGATCCAGAAAATTACTCAATTTGGTATGGTGAATATAAATATCCAGAAGAATT
GACTAAGGTATTTATGAGCTGCAATTTAATCACCGGTATGTTCCAACGTCTTGATAAAAT
GAGAAAACAAGCATTTGCCTCATGCTGCCTCTTCGGTGAAGACAATAATAGCACAATTTC
TGGTGTCTGGGTATGGCGTGGACAAGAGCTTGCATTTGGACTTTCACCAGATTGGCAAAT
CGATTACGAGAGCTATGATTGGAAGAAATTAGATCCATCCAGTGAAGAAACTAAGAAATT
GGTAACACAATATTTCTCATGGACTGGAACTGACAAAGGAGGCCGCAAATTCAATCAAGG
CAAAATTTTCAAATAA
>g714.t20 Gene=g714 Length=432
MLLYTYPENFRAYKALIAAQYSGAKVDIAPNFVFGETNRTESFLKKFPLGKVTLETDDGK
YLAESNAIAYYVSNAQLRGASDFEKAEVLQWLSFADNEIGPAAAAWVYPALGYIQFNKLS
HERAKQDIARSLERLQARLVNNTYLVGERITLADIVVFTSLLSLFQHVADPAYRKPFFAV
NRWFNTILNQPQTKAVVKNFTFCEKETPFDPKKYAEFQAKFGGGAQQKEKKEKEKKEKQP
QQPAAEKKKKEKEAEPAEEMDEAELALAAEPKSKDPFDSLPKGTFNMDDFKRVYSNEDEA
KSIPYFFEKFDPENYSIWYGEYKYPEELTKVFMSCNLITGMFQRLDKMRKQAFASCCLFG
EDNNSTISGVWVWRGQELAFGLSPDWQIDYESYDWKKLDPSSEETKKLVTQYFSWTGTDK
GGRKFNQGKIFK
Protein features from InterProScan
| Transcript | Database | ID | Name | Start | End | E.value | |
|---|---|---|---|---|---|---|---|
| 13 | g714.t20 | CDD | cd03044 | GST_N_EF1Bgamma | 3 | 74 | 4.84992E-27 |
| 12 | g714.t20 | CDD | cd03181 | GST_C_EF1Bgamma_like | 84 | 206 | 3.7397E-61 |
| 11 | g714.t20 | Gene3D | G3DSA:3.40.30.10 | Glutaredoxin | 1 | 72 | 1.7E-20 |
| 9 | g714.t20 | Gene3D | G3DSA:1.20.1050.10 | - | 74 | 211 | 1.1E-47 |
| 10 | g714.t20 | Gene3D | G3DSA:3.30.70.1010 | - | 273 | 432 | 1.1E-75 |
| 15 | g714.t20 | MobiDBLite | mobidb-lite | consensus disorder prediction | 223 | 262 | - |
| 16 | g714.t20 | MobiDBLite | mobidb-lite | consensus disorder prediction | 225 | 256 | - |
| 4 | g714.t20 | PANTHER | PTHR43986:SF1 | ELONGATION FACTOR 1-GAMMA | 2 | 432 | 1.7E-169 |
| 5 | g714.t20 | PANTHER | PTHR43986 | ELONGATION FACTOR 1-GAMMA | 2 | 432 | 1.7E-169 |
| 3 | g714.t20 | Pfam | PF02798 | Glutathione S-transferase, N-terminal domain | 3 | 73 | 7.2E-13 |
| 2 | g714.t20 | Pfam | PF00043 | Glutathione S-transferase, C-terminal domain | 107 | 191 | 1.9E-13 |
| 1 | g714.t20 | Pfam | PF00647 | Elongation factor 1 gamma, conserved domain | 272 | 377 | 3.3E-48 |
| 18 | g714.t20 | ProSiteProfiles | PS50404 | Soluble glutathione S-transferase N-terminal domain profile. | 1 | 80 | 14.776 |
| 17 | g714.t20 | ProSiteProfiles | PS50405 | Soluble glutathione S-transferase C-terminal domain profile. | 81 | 209 | 23.448 |
| 19 | g714.t20 | ProSiteProfiles | PS50040 | Elongation factor 1 (EF-1) gamma C-terminal domain profile. | 273 | 432 | 84.258 |
| 20 | g714.t20 | SFLD | SFLDS00019 | Glutathione Transferase (cytosolic) | 1 | 187 | 3.5E-25 |
| 14 | g714.t20 | SMART | SM01183 | EF1G_2 | 272 | 377 | 1.3E-76 |
| 7 | g714.t20 | SUPERFAMILY | SSF52833 | Thioredoxin-like | 1 | 74 | 5.25E-11 |
| 8 | g714.t20 | SUPERFAMILY | SSF47616 | GST C-terminal domain-like | 74 | 204 | 5.01E-31 |
| 6 | g714.t20 | SUPERFAMILY | SSF89942 | eEF1-gamma domain | 273 | 432 | 9.29E-70 |
Transmembrane regions from TMHMM
Disordered region
IUPRED3 score over 0.5 is predictive of a disordered region.
GO terms from InterProScan
| GOID | TERM | ONTOLOGY |
|---|---|---|
| GO:0006749 | glutathione metabolic process | BP |
| GO:0005515 | protein binding | MF |
| GO:0003746 | translation elongation factor activity | MF |
| GO:0006414 | translational elongation | BP |
KEGG
Orthology
- This transcript has been annotated with K03233 (elongation factor 1-gamma)
Pathway
- This transcript belongs to the following pathways
Expression
Transcript expression in Pv11 cells
TPM values are indicated as average +/- STDEV. 
Differential expression
Differentially expressed genes were identified with DESeq2 using the ‘run_DE_analysis.pl’ script from Trinity. Transcripts were determined as differentially expressed when (1) FDR < 0.05 (2) fold change > 2 (TPM calculated by RSEM). DE information and fold change between conditions are indicated in the plot below.
